lncRNA EPB41L4A-AS通过调控ErbB3抑制奶牛乳腺上皮细胞增殖的研究

doi:10.11843/j.issn.0366-6964.2022.07.012

Abstract

Abstract: This study aimed to explore the function and regulatory mechanism of EPB41L4A-AS with high expression in early lactation in bovine mammary epithelial cells (BMECs). In this study, EPB41L4A-AS siRNA and si-NC were transfected into BMECs, respectively. The effect of EPB41L4A-AS siRNA on the proliferation of BMECs was detected by EdU and MTT assays; qPCR and Western blot methods were used to detect the expression of ErbB3, PIK3R1, AKT and STAT5a genes. EdU and MTT results showed that the activity of BMECs was significantly increased with the interference of EPB41L4A-AS expression (P<0.001) and the number of EdU positive cells obviously increased. qPCR and Western blot results showed that the relative mRNA abundance of ErbB3, PIK3R1, AKT and STAT5a were significantly higher than those of the control group after interfering EPB41L4A-AS expression (P<0.05), and the relative protein expression levels of ErbB3, PIK3R1, AKT, p-AKT and STAT5a significantly increased (P<0.05). The results of this study suggested that reducing the expression of EPB41L4A-AS promoted the expression of ErbB3, and activated the PI3K/AKT and JAK-STAT signaling pathways to promote the proliferation of BMECs. This study revealed the role and regulatory pathway of EPB41L4A-As in BMECs proliferation, which provided a theoretical basis for further exploring the regulation mechanism of lncRNA on BMECs.

Key words: lncRNA, ErbB3, signaling pathway, bovine mammary epithelial cells

CLC Number:

S823.2

LIU Lihua, ZHONG Zhenyu, ZHENG Yujie, WANG Xin. lncRNA EPB41L4A-AS Inhibits the Proliferation of Bovine Mammary Epithelial Cells by Regulating the Expression of ErbB3 Gene[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2152-2159.

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lncRNA EPB41L4A-AS Inhibits the Proliferation of Bovine Mammary Epithelial Cells by Regulating the Expression of ErbB3 Gene

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