鸡TGF-β1对大肠杆菌和鸡白痢沙门菌黏附DF1细胞的影响

doi:10.11843/j.issn.0366-6964.2022.11.025

Abstract

Abstract: The aim of this experiment was to study the effects of chicken transfer growth factor-β1 (TGF-β1) on the adhesion of Escherichia coli and Salmonella pullorum to DF1 cells. The expression of chicken TGF-β1 was detected by ELISA after DF1 cells were infected with Newcastle disease virus (NDV), then the overexpression and inhibitory expression vectors of chicken TGF-β1 were constructed by referring to the chicken TGF-β1 sequence in GenBank. The transfection efficiency of the recombinant TGF-β1 expression vector was observed under fluorescence microscope 48 h after transfection of DF1 cells. The mRNA level changes of chicken TGF-β1 in DF1 cells were detected by fluorescence quantitative PCR, and the changes of chicken TGF-β1 extracellar protein expression level were detected by ELISA. The effects of chicken TGF-β1 on the adhesion of pathogenic Escherichia coli and Salmonella pullorum to DF1 cells were detected by adhesion assay. The results showed that the extracellular expression level of TGF-β1 in DF1 cells infected with NDV was significantly higher than that in uninfected cells (P<0.05). Enzyme digestion and sequencing confirmed that the recombinant vectors with TGF-β1 inhibitory expression and overexpression were successfully constructed. The expression levels of TGF-β1 mRNA and extracellular protein in transfected chicken TGF-β1 recombinant overexpressed vector cells were significantly higher than those of untransfected cells (P<0.01), and the adhesion rates of pathogenic Escherichia coli and Salmonella pullorum to cells were significantly higher than those of untransfected cells (P<0.01). The expression levels of TGF-β1 mRNA and extracellular protein in transfected chicken TGF-β1 recombinant inhibitory vector cells were significantly lower than those of untransfected cells (P<0.01), and the adhesion rates of pathogenic Escherichia coli and Salmonella pullorum to cells were significantly lower than those of untransfected cells (P<0.01). In conclusion, the expression level of chicken TGF-β1 increased after NDV infection of DF1 cells, and chicken TGF-β1 can promote the adhesion of pathogenic Escherichia coli and Salmonella pullorum to DF1 cells, which provides experimental basis for further study on the role of chicken TGF-β1 in the bacterial disease secondary to poultry virus infection.

Key words: chicken, TGF-β1, Escherichia coli, Salmonella pullorum, adhesion

CLC Number:

S852.612

MEN Kaikai, LIU Jialong, GUO Yage, HE Lei, JIA Yanyan, YU Zuhua. Effects of Chicken TGF-β1 on the Adhesion of Escherichia coli and Salmonella pullorum to DF1 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4000-4007.

References 24

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Effects of Chicken TGF-β1 on the Adhesion of Escherichia coli and Salmonella pullorum to DF1 Cells

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