杜洛克与二花脸杂交猪群体SNP偏分离分析

doi:10.11843/j.issn.0366-6964.2021.09.002

Abstract

Abstract: The phenomenon of transmission ratio distortion (TRD) is commonly observed in many organisms, and the purpose of this study is to excavate isolated porcine genomic marker sites and explore their potential genetic mechanisms in a large population with high density microarray to determine genotype. In this study, 60K Illumina Porcine SNP chip was used to genotype 1 020 F2 resource families (Duroc×Erhualian intercross F0-F2 populations). The transmission ratio distortion signal was detected at a single site by TRDscan (BF>100) and TDT (P<0.01), and the function of genes within 100 kb window near the significant signal site shared by the two were analyzed. In addition, a software package for haplotype typing PHASEBOOK was used to analyze the TRD region on paternal and maternal gametes, and multi-loci linkage analysis was utilized to search for potential QTLs within the region. The results showed that:1) In TRD analysis of single site, 44 significant TRD sites were identified and 23 related genes were screened; 27 and 35 significant TRD loci were identified by analyzing paternal and maternal specific TRD effects, among them, there were annotated functional genes near 100 kb of 11 and 25 loci, respectively. 2) Based on the TRD results of haplotype multi-site linkage, the significant paternal TRD sites were located on chromosome 5 and 13, and functionally related QTLs were searched in this TRD region, then 3 QTLs were found to be related to reproductive traits (number of mummies, number of live litters, and number of corpus luteum); The significant maternal TRD regions were located on chromosome 4, 6 and 12, combined with the known QTL database of pigs, 5 QTLs related to reproductive traits were found. The study used large-scale pig family data to systematically identify the TRD sites of pig genomes, which will provide a certain reference for analyzing the TRD phenomenon in pigs and further exploring its biological mechanism.

Key words: transmission ratio distortion, haplotype, GO enrichment analysis, QTL

CLC Number:

S828.2

CHEN Zuoquan, RAO Lin, XIE Lei, YAO Tianxiong, ZHANG Zhiyan. Transmission Ratio Distortion Analysis of SNP in a Duroc×Erhualian Intercross Pigs Population[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2384-2393.

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Transmission Ratio Distortion Analysis of SNP in a Duroc×Erhualian Intercross Pigs Population

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