披针叶黄华内生真菌的分离与鉴定

doi:10.11843/j.issn.0366-6964.2021.010.030

Abstract

Abstract: To investigate the endophytic fungi species and population distribution of the poisonous plant, Thermopsis lanceolata, the experimental materials collected from Qinghai Province were disinfected with surface disinfection method and then endophytic fungi were separated. The morphological and 5.8S rDNA-ITS sequence analysis were used to identify these strains, and the MEGA7.0 software was used for phylogenetic analysis. The results showed that twenty-nine endophytic fungi were isolated from the Thermopsis lanceolata, which belong to 7 classes, 9 orders, 11 families and 12 genera. The total relative separation frequency was 85.42%. Endophytic fungi are most commonly isolated from leaves (41.38%), followed by stems (37.93%), seed (13.79%) and roots (6.90%). According to phylogenetic analysis, all endophytic fungi belong to two populations with close genetic relationship. The results indicated that Thermopsis lanceolata had abundant endophytic fungi species and Penicillium sp. was the dominant genus.

Key words: Thermopsis lanceolata, endophytic fungi, isolation and identification, phylogenetic analysis, Penicillium sp.

CLC Number:

S859.87

ZHAO Wenxing, WU Kexin, GUO Ziyu, ZHANG Yunhao, TANG Lihui, LIU Yiling, MO Chonghui, ZHAO Baoyu, LU Hao. Isolation and Identification of Endophytic Fungi in Thermopsis lanceolata[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2986-2994.

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Isolation and Identification of Endophytic Fungi in Thermopsis lanceolata

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