影响马胚胎移植成功率的关键因素分析

doi:10.11843/j.issn.0366-6964.2020.01.010

Abstract

Abstract: This study aimed to research factors affecting the efficiency of equine embryo transfer. The data of embryo transfer in 3 horse farms from 2013 to 2018 were analyzed.The number of donor horses in the 3 farms were 15, 21 and 25, respectively, and the number of recipient mares were 56, 50 and 75 respectively. All mares were 3 to 12 years old. The following items were counted:The effect of flushing day post ovulation of donor horses on the embryo recovery rate; The effect of embryonic age on the pregnancy rate of recipient mares after transplantation; The effect of the degree of ovulation synchronization of the donor and recipient mares on the pregnancy rate after transplantation; The effect of residence time at farm of recipient mares on pregnancy rate after transplantation. The results indicated that, mares were injected with prostaglandin (PG)+GnRH analogues or PG + hCG to induce ovulation during the breeding season, the estrous cycle were (14.5±0.8) and (14.3±1.1)d, respectively, which was significantly lower than the control group ((20.5±2.6) d, P<0.05). The embryo recovery rate of washing the uterus on the 8th day after ovulation was higher than that on the 7th day, but the difference was not significant; The pregnancy rate of recipient horses after 8-day-old embryo transfer was higher than that of 7-day-old, and the difference was not significant; After embryo tranfer, the recipients which ovulated 1 day later than donors had the highest pregnancy rate. When the residence time at farm of recipients was longer than 1 year, the pregnancy rate was significantly higher than those shorter than 0.5 year. In conclusion, the injection of PG + hCG or PG + GnRH anologue could shorten estrus cycle of mares. The embryo recovery rate and the pregnancy rate after transplantation of the 8th day embryo after ovulation were higher. When the embryos were transplanted, the mares with the residence time at farm more than 1 year and the ovulation time 1 day later than the donors were used as recipients.

Key words: horse, embryo transfer, pregnancy rate

CLC Number:

S821.3

LI Nan, WANG Tao, ZHANG Fenglong, ZHANG Xiang, LI Pingsui, HAN Guocai, ZENG Shenming. The Analysis of Key Factors Affecting the Success Rate of Equine Embryo Transfer[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 83-89.

References 36

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The Analysis of Key Factors Affecting the Success Rate of Equine Embryo Transfer

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