蒙古斑点马黑、白毛色区域皮肤中相关基因的表达研究

doi:10.11843/j.issn.0366-6964.2022.01.010

Abstract

Abstract: In this study, phenotypes and differentially expressed genes in the white and black regions of the body of Mongolian spotted horse were analyzed and verified, in an attempt to elucidate the molecular mechanism of hair color formation of Mongolian spotted horse, and lay a foundation for future protection, development and utilization of Mongolian spotted horse. First, skin tissue sections of the white and black regions of the body of Mongolian spotted horse were made and stained by HE staining method and phenotypic differences were observed under a microscope. Secondly, transcriptome sequencing was performed on the skin of white and black regions to compare the differentially expressed mRNAs. Finally, the selected typical genes were tested by immunofluorescence immunohistochemistry and qRT-PCR, the results of transcriptome sequencing were further verified. The results showed that there were significant differences in the pigment distribution between the black and white skin tissues of Mongolian spotted horses. Melanocytes were mainly distributed in the epidermis and hair follicle parts of the black skin tissues, while no melanin deposition was found in the corresponding positions of the white skin tissues. The expression levels of 740 genes were significantly different between black and white skin tissues (P<0.05), among which 109 genes were highly expressed in black skin tissue of Mongolian spotted horse, and 215 genes were highly expressed in white skin tissue of Mongolian spotted horse. Differentially expressed mRNAs were mainly enriched in melanin production pathway contained TYR, TYRP1, DCT, PAX3 and DAPL1, etc.genes. TYR and TYRP1 proteins were not expressed in the hair bulb of the hair follicle of Mongolian spotted horse. DCT was expressed in the mother material of hair follicle. The hair color formation of Mongolian spotted horse is mainly determined by the expression of genes related to melanin formation.

Key words: Mongolian spotted horse, skin tissue, differentially expressed mRNA

CLC Number:

S821.81.2

BAI Dongyi, LA Xima, ZHAO Ruoyang, TAO Nala, HAN Haige, DING Wenqi, JIA Zijie, LIU Huiying, WANG Wenxing, HUANG Boguang, MANG Lai. Study on the Expression of Related Genes in the Skin of Mongolian Spotted Horses in Black and White Coat Regions[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 100-111.

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Study on the Expression of Related Genes in the Skin of Mongolian Spotted Horses in Black and White Coat Regions

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