呼伦贝尔羊重要脂肪相关基因mRNA差异表达分析

doi:10.11843/j.issn.0366-6964.2019.09.022

Abstract

Abstract: Based on the transcriptome sequencing results of tail adipose tissue between the big-tailed and small-tailed Hulun Buir sheep, 10 important genes related to fat were selected to explore their expression in different tissues and investigate the relationship between genes and fat metabolism in sheep. The mRNA expression difference of candidate genes, including CFD, PLIN4, THY1, IL-18, PTPN11, LPL, IRX3, HOXA10, MID1IP1 and UGCG, were detected in 7 tissues (tail fat, hip fat, subcutaneous fat, perirenal fat, omental fat, liver and muscle) between big-tailed and small-tailed Hulun Buir sheep of 6-month-old by real-time fluorescence quantitative method. The results showed that:1) Ten genes were expressed in other tissues except for trace expression in muscle, and there was a significant difference at the expression levels in one or more tissues between different lines. 2) The expression levels of IRX3 and UGCG genes in the tail adipose tissue of the big-tailed sheep were significantly lower than those in the small-tailed sheep (P<0.01), indicating that these 2 genes were directly related to fat metabolism in the tail of sheep. 3) The expression trends of PLIN4 and PTPN11 genes in different tissues were similar, and the expression levels in subcutaneous and omental adipose tissues in the big-tailed sheep were higher than those in the small-tailed sheep (P<0.01), while the expression trend of THY1 gene was opposite, the expression in subcutaneous and omental fat tissues in the big-tailed sheep was lower than that in the small-tailed sheep (P<0.01, P<0.05). 4) The expression of LPL gene in the hip adipose tissue of the small-tailed sheep was significantly higher than that of the big-tailed sheep (P<0.01); only the CFD gene was highly expressed in the liver and higher in the big-tailed sheep than that in the small-tailed sheep (P<0.01), while other genes were lowly or tracely expressed in the liver; HOXA10 gene was highly expressed only in perirenal adipose tissue, and the expression level in big-tailed sheep was higher than that in small-tailed sheep (P<0.01). The results will provide a reference for further elucidate the molecular mechanism of fat metabolism in sheep.

CLC Number:

S826.2

DENG Tianyu, FAN Hongying, DU Lixin, ZHAO Fuping, WANG Lixian. Differential Expression Analysis of Important Candidate Genes mRNA Related to Fat in Hulun Buir Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1926-1935.

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Differential Expression Analysis of Important Candidate Genes mRNA Related to Fat in Hulun Buir Sheep

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