牦牛HDAC1基因克隆及其在组织和卵母细胞减数分裂过程中的表达研究

doi:10.11843/j.issn.0366-6964.2019.10.005

Abstract

Abstract: The objective of this research was to clone the histone deacetylases 1 (HDAC1) gene of yak and identify its expression patterns in various tissues and oocytes during meiosis process, respectively, which would provide theoretical basis for studying the role mechanism of HDAC1 in reproductive development of yak. The samples of yak heart, liver, spleen, lung, kidney, small intestine, brain, muscle, ovary and uterus were collected after slaughtering. Total RNAs in different samples were extracted. The coding sequence of HDAC1 gene was cloned by RT-PCR. Its structure and function were analyzed by bioinformatics softwares. The mRNA expression of HDAC1 in different tissues and oocyte during meiosis process was detected by quantitative real-time PCR (qRT-PCR). The results showed that the open reading frame HDAC1 was 1 302 bp, encoding 433 amino acids. It had high homology with cattle, sheep and goat. HDAC1 gene of yak was widely expressed in various tissues, the expression level of which was significantly higher in spleen, kidney and small intestine(P<0.01). The expression level of HDAC1 in MI and MⅡ stages was significantly higher than that in GV stage in oocytes of yak(P<0.01). This study suggested that HDAC1 was involved in meiosis process of oocytes in yak, which provided basic data for studying the role of HDAC1 in reproductive development of yak under the harsh natural environment of the plateau.

CLC Number:

S823.85

WANG Bin, YIN Shi, XIONG Xianrong, QIN Wenchang, HUANG Xiangyue, LI Jian. Cloning of Yak HDAC1 Gene and Its Expression Pattern in Tissues and Oocyte during the Process of Meiosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 1997-2004.

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References 34

[1]	REID M A,DAI Z W,LOCASALE J W.The impact of cellular metabolism on chromatin dynamics and epigenetics[J].Nat Cell Biol,2017,19(11):1298-1306.
[2]	VERDIN E,DEQUIEDT F,KASLER H G.Class Ⅱ histone deacetylases:versatile regulators[J].Trends Genet,2003,19(5):286-293.
[3]	ECKSCHLAGER T,PLCH J,STIBOROVA M,et al.Histone deacetylase inhibitors as anticancer drugs[J].Int J Mol Sci,2017, 18(7):1414.
[4]	GU L,WANG Q,SUN Q Y.Histone modifications during mammalian oocyte maturation:dynamics,regulation and functions[J]. Cell Cycle, 2010,9(10):1942-1950.
[5]	KAGEYAMA S I,LIU H L,KANEKO N,et al.Alterations in epigenetic modifications during oocyte growth in mice[J]. Reproduction, 2007,133(1):85-94.
[6]	WU X,LI Y,XUE L,et al.Multiple histone site epigenetic modifications in nuclear transfer and in vitro fertilized bovine embryos[J]. Zygote,2011,19(1):31-45.
[7]	WANG H L,CUI W,MENG C H,et al.MC1568 enhances histone acetylation during oocyte meiosis and improves development of somatic cell nuclear transfer embryos in pig[J].Cell Reprogram,2018,20(1):55-65.
[8]	TANG L S,WANG Q,XIONG B,et al.Dynamic changes in histone acetylation during sheep oocyte maturation[J].J Reprod Dev, 2007,53(3):555-561.
[9]	VE?E?A J,BÁRTOVÁ E,KREJ?Í J,et al.HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals[J].J Cell Physiol,2018,233(1):530-548.
[10]	KIWELER N,BRILL B,WIRTH M,et al.The histone deacetylases HDAC1 and HDAC2 are required for the growth and survival of renal carcinoma cells[J].Arch Toxicol,2018,92(7):2227-2243.
[11]	蔡雯祎,熊显荣,陈通,等.KDM2B基因克隆及其在牦牛组织和卵母细胞减数分裂过程中的表达研究[J].畜牧兽医学报,2018,49(3):534-541.
	CAI W Y,XIONG X R,CHEN T,et al.Molecular cloning of KDM2B gene and its expression pattern in tissues and the process of oocyte meiosis in yak[J].Acta Veterinaria et Zootechnica Sinica,2018,49(3):534-541.(in Chinese)
[12]	韩杰,熊显荣,王艳,等.牦牛KDM1A基因克隆及其在不同发育时期睾丸中的表达规律[J].畜牧兽医学报,2018, 49(12):2777-2785.
	HAN J,XIONG X R,WANG Y,et al.Cloning of yak KDM1A gene and its expression profile in testis during different developmental stages[J] Acta Veterinaria et Zootechnica Sinica,2018,49(12):2777-2785.(in Chinese)
[13]	WANG Z,ZHAO T,ZHANG P,et al.Histone deacetylase 1 down-regulation on developmental capability and histone acetylation in bovine oocytes and parthenogenetic embryos[J].Reprod Domest Anim,2011,46(6):1022-1028.
[14]	SEN N.Epigenetic regulation of memory by acetylation and methylation of chromatin:implications in neurological disorders, aging, and addiction[J].Neuromolecular Med,2015,17(2):97-110.
[15]	MILSTONE Z J,LAWSON G,TRIVEDI C M.Histone deacetylase 1 and 2 are essential for murine neural crest proliferation, pharyngeal arch development,and craniofacial morphogenesis[J].Dev Dyn,2017,246(12):1015-1026.
[16]	MA P,SCHULTZ R M.HDAC1 and HDAC2 in mouse oocytes and preimplantation embryos:specificity Versus compensation[J]. Cell Death Differ,2016,23(7):1119-1127.
[17]	PENG M,LI Y,HUANG H,et al.The expression of GCN5,HDAC1 and DNMT1 in parthenogenetically activated mouse embryos[J]. J Obstet Gynaecol,2015,35(2):131-135.
[18]	ARCIDIACONO O A,KREJC'Í J,SUCHÁNKOVÁ J,et al.Deacetylation of histone H4 accompanying cardiomyogenesis is weakened in HDAC1-depleted ES cells[J].Int J Mol Sci,2018,19(8):2425.
[19]	DATTA M,STASZEWSKI O,RASCHI E,et al.Histone deacetylases 1 and 2 regulate microglia function during development, homeostasis,and neurodegeneration in a context-dependent manner[J].Immunity,2018,48(3):514-529.
[20]	JOUNG H,KWON S,KIM K H,et al.Sumoylation of histone deacetylase 1 regulates MyoD signaling during myogenesis[J].Exp Mol Med,2018,50(1):e427
[21]	FAULKNER B,ASTLEFORD K,MANSKY K C.Regulation of osteoclast differentiation and skeletal maintenance by histone deacetylases[J].Molecules,2019,24(7):1355.
[22]	SONG Y C,DOHN T E,RYDEEN A B,et al.HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development[J].PLoS Genet,2019,15(5):e1008165.
[23]	FELLOWS R,DENIZOT J,STELLATO C,et al.Microbiota derived short chain fatty acids promote histone crotonylation in the colon through histone deacetylases[J].Nat Commun,2018,9(1):105.
[24]	DOVEY O M,FOSTER C T,COWLEY S M.Histone deacetylase 1(HDAC1),but not HDAC2,controls embryonic stem cell differentiation[J].Proc Natl Acad Sci U S A,2010,107(18):8242-8247.
[25]	WANG J,HUANG N N,XIONG J,et al.Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase inhibition[J].Int Immunopharmacol,2018,65(2018):303-311.
[26]	ZIMMERMANN S,KIEFER F,PRUDENZIATI M,et al.Reduced body size and decreased intestinal tumor rates in HDAC2-mutant mice[J].Cancer Res,2007,67(19):9047-9054.
[27]	HUANG J,DING C H,LI Z Y,et al.Epigenetic changes of histone deacetylation in murine oocytes matured in vitro versus in vivo[J].Eur Rev Med Pharmacol Sci,2017,21(9):2039-2044.
[28]	BRUNMEIR R,LAGGER S,SEISER C.Histone deacetylase HDAC1/HDAC2-controlled embryonic development and cell differentiation[J].Int J Dev Biol,2009,53(2-3):275-289.
[29]	MINUCCI S,PELICCI P G.Histone deacetylase inhibitors and the promise of epigenetic (and more) treatments for cancer[J]. Nat Rev Cancer,2006,6(1):38-51.
[30]	KRIVORUCHKO A,STOREY K B.Epigenetics in anoxia tolerance:a role for histone deacetylases[J].Mol Cell Biochem, 2010, 342(1-2):151-161.
[31]	LUO W B,WANG Y F.Epigenetic regulators:multifunctional proteins modulating hypoxia-inducible factor-α protein stability and activity[J].Cell Mol Life Sci,2018,75(6):1043-1056.
[32]	KIM S H,JEONG J W,AE PARK J,et al.Regulation of the HIF-1α stability by histone deacetylases[J].Oncol Rep,2007, 17(3):647-651.
[33]	QIAN D Z,KACHHAP S K,COLLIS S J,et al.Class Ⅱ histone deacetylases are associated with VHL-independent regulation of hypoxia-inducible factor 1α[J].Cancer Res,2006,66(17):8814-8821.
[34]	ZHANG L B,BU L,HU J,et al.HDAC1 knockdown inhibits invasion and induces apoptosis in non-small cell lung cancer cells[J]. Biol Chem,2018,399(6):603-610.

Cloning of Yak HDAC1 Gene and Its Expression Pattern in Tissues and Oocyte during the Process of Meiosis

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