热应激造成奶牛乳腺上皮细胞损伤并影响乳合成相关载体的基因表达

doi:doi: 10.11843/j.issn.0366-6964.2016.08.023

Abstract

Abstract:

Bovine mammary epithelial cell was chosen as the model to find the factors impairing lactation performance of dairy cow during heat stress.Cell vitality，mitochondrial membrane potential and the expression of genes related to heat shock molecule and proteins，oxidative stress，amino acids and glucose transporters were detected in the bovine mammary epithelial cells treated for 0，0.5，1，1.5，2，4，8 and 12 h at 42 ℃，respectively.The result showed that different heat treatment time significantly reduced mammary epithelial cell vitality (P<0.05) and damaged mitochondrial membrane potential.The expression of HSP27 decreased significantly (P<0.05)，while HSP70 increased significantly (P<0.05).The expression of HSP90 increased significantly in 0-4 h (P<0.05)，and returned to normal level after then.The expression of HSF-1 increased significantly in some time points (P<0.05).The gene expression of Keach like ECH-associated protein-1 (Keap1) showed no significant difference(P>0.05)，while nuclear factor-E2-related factor 2 (Nrf2) and NAD (P) H dehydrogenase quinone-1 (NQO1) increased significantly (P<0.05).The gene expression of transmembrane amino acid transporter SLC7A7 increased significantly in 1 h and 4 h(P<0.05).The gene expression of glucose transporters GLUT1 and GLUT8 decreased significantly (P<0.05).The expression of glucose transporter GLUT12 increased significantly during 0-2 h，and decreased significantly during 4-12 h (P<0.05).In conclusion，heat stress could significantly reduce the cell vitality，cause oxidative stress，change the gene expression of heat shock proteins and molecule and membrane transport carriers.

CLC Number:

S852.23

QUAN Su-yu，ZHANG Yuan-shu，BU Deng-pan . Heat Stress-induced Cell Injury and Effects on the Gene Expression of Milk Synthesis-related Transporters in Dairy Cow[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1704-1713.

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Heat Stress-induced Cell Injury and Effects on the Gene Expression of Milk Synthesis-related Transporters in Dairy Cow

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