ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (8): 1714-1719.doi: doi: 10.11843/j.issn.0366-6964.2016.08.024

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Histological Comparison and mRNA Expression Analysis of Porcine Seminal Protein on Accessory Glands of Duroc and Meishan Boars

DING Hai-sheng 1,XU De-quan 1,2,3,LUO Yan 1,LIU Min 1,2*   

  1. (1.College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,
    China;2.Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry,Wuhan 430070,China;
    3.The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,China)
  • Received:2016-01-29 Online:2016-08-23 Published:2016-08-23

Abstract:

In order to assess the histological features of the vesicular gland and bulbourethral gland,and the mRNA expression of porcine seminal protein in 270-day-old Duroc and 75-,270- day-old Meishan boars,the paraffin sections with HE staining were performed and observed under light microscope,and quantitative real-time PCR was also used.Increased glandular lobules and decreased interlobular connective tissues were found in the vesicular glands of 270-day-old Meishan boars compared to those of 75-day-old Meishan boars.More acinus mucosal folds and interlobular connective tissues were found in the vesicular glands of 270-day-old Duroc boars than those of 270-day-old Meishan boars.The bulbourethral glands were composed of multiple lobules separated by connective tissue,and dense acini with small lumens were found.When Meishan boars reached adult age,the enlarged glandular lobules and decreased interlobular connective tissues were observed in the bulbourethral glands.In addition,larger glandular lobules,smaller acini and less interlobular connective tissues were observed in the bulbourethral glands of 270-day-old Meishan boars than those of 270-day-old Duroc boars.PSP-Ⅰ and PSP-Ⅱ were expressed highly in seminal vesicles,but there was no significant difference between the mature Duroc and Meishan boars (P>0.05).With advancing age,the mRNA expression level of PSP-Ⅰ and PSP-Ⅱ increased significantly in Meishan boars (P<0.05).Overall,more acinus mucosal folds in vesicular glands and larger acini in the bulbourethral glands of Duroc boars increased the epithelial surface area available for secretion and led to an increase in the semen volume and the rate of sperm metabolism and survival.Moreover,high expression of PSP-Ⅰ and PSP-Ⅱ in mature boars may play a role in some physiological processes,such as maintaining sperm metabolism and motility.

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