猫牙周炎相关口腔菌群分析及防治效果观察

doi:10.11843/j.issn.0366-6964.2025.08.043

Abstract

Abstract:

Periodontitis refers to a series of inflammatory lesions caused by dental plaque that damage the periodontal tissue and its supporting structures. It is a common chronic, non-communicable disease in feline. At present, the research on cat periodontitis in China is relatively insufficient, and there is a lack of scientific prevention and control methods. In this study, samples from different areas of the oral cavity of healthy cats and cats with periodontitis were collected, and microbial species identification and antimicrobial susceptibility tests were carried out to provide medication recommendations for clinical treatment.The bacteriostatic effect of commonly used antibacterial agents after surgical cleaning was evaluated in order to provide a theoretical basis for the treatment of periodontitis in cats and home oral care. The results showed that, in all cats tested, Subgingival microbial species abundance is higher than supragingival ones. The abundance of oral microbial species in cats with periodontitis is higher than that in healthy cats. Porphyromonas and Treponema are higher in subgingival than supragingival. As potential pathogenic microorganisms, it is speculated that they may be the key pathogenic factors of periodontitis. Results showed that the oral microbiota of cats with periodontitis was more sensitive to Enrofloxacin and less sensitive to Metronidazole. In three oral care products with the antimicrobial peptides, the chlorhexidine gluconate and the silver ion as the main components respectively, the chlorhexidine gluconate and the silver ion have better bacteriostatic effect. But the chlorhexidine gluconate tastes bitter and has poor palatability for cats.It is recommended to be used as an oral antiseptic in surgery. In summary, Porphyromonas and Treponema may be the main pathogens of feline periodontitis. Enrofloxacin can be used as an empirical adjunctive therapy for feline periodontitis. It is recommended to use the Chlorhexidine gluconate in ultrasonic scaling, and to use the Silver ions after surgery.

Key words: feline, periodontitis, microbial species identification, antimicrobial susceptibility test

CLC Number:

S857.14

ZHANG Xinyu, DAI Pengxiu, ZHANG Fan, ZHANG Xinke. The Relationship between Cat Periodontal Disease and Oral Flora and Its Prevention and Treatment[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(8): 4065-4073.

Figures/Tables 8

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References 12

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药品及试剂 Drugs and reagents	生产厂家 Manufacturer
恩诺沙星细菌药敏试纸/(5 μg·片^－1) Enrofloxacin bacterial susceptibility test strips	杭州滨和微生物试剂有限公司
氨苄西林细菌药敏试纸/(10 μg·片^－1) Ampicillin bacterial susceptibility test strips	杭州滨和微生物试剂有限公司
头孢氨苄细菌药敏试纸/(30 μg·片^－1) Cephalexin bacterial susceptibility test strips	杭州滨和微生物试剂有限公司
阿莫西林细菌药敏试纸/(10 μg·片^－1) Amoxicillin bacterial susceptibility test strips	杭州滨和微生物试剂有限公司
多西环素细菌药敏试纸/(30 μg·片^－1) Doxycycline bacterial susceptibility test strips	杭州滨和微生物试剂有限公司
阿奇霉素细菌药敏试纸/(15 μg·片^－1) Azithromycin bacterial susceptibility test strips	杭州滨和微生物试剂有限公司
甲硝唑细菌药敏试纸/(5 μg·片^－1) Metronidazole bacterial susceptibility test strips	常德比克曼生物科技有限公司
抗菌肽口腔喷剂 Antimicrobial peptides	绵阳天鸿生物科技有限公司
活性银离子抗菌液 Silver ion	西安映希生物科技有限公司
0.2%葡萄糖酸氯己定黏膜冲洗消毒液0.2% Chlorhexidine gluconate	山东利尔康有限公司
LB肉汤 Luria-Bertani Broth	青岛高科技工业园海博生物技术有限公司
Mueller-Hinton琼脂 Mueller-Hinton Agar	青岛高科技工业园海博生物技术有限公司
血平板培养基 Blood plating medium	广东环凯微生物科技有限公司
厌氧产气袋 AnaeroPack	日本三菱瓦斯化学株式会社
厌氧指示剂 Suitable for microbiology	美国赛默飞世尔(Thermo Fisher Scientific)科技公司
异氟烷 Isoflurane	瑞普(天津)生物药业有限公司
丙泊酚 Propofol	广东嘉博制药有限公司

仪器设备 Instrument	生产厂家 Manufacturer
呼吸麻醉机 Respiratory anesthesia machine	深圳迈瑞(Mindray)动物医疗科技有限公司
心电监护仪 ECG monitor	深圳迈瑞(Mindray)动物医疗科技有限公司
牙科工作台 Dental workstations	古氏(KRUUSE)贸易(上海)有限公司
牙科刮匙 Bone curettes	古氏(KRUUSE)贸易(上海)有限公司
牙科探针 Dental explorer	古氏(KRUUSE)贸易(上海)有限公司
普通冰箱 Refrigerator	青岛海尔(Haier)股份有限公司
高压灭菌锅 Autoclave	上海博讯医疗生物仪器股份有限公司
超净工作台 Clean bench	苏州净化设备有限公司
微量移液器 Pipettor	美国赛默飞世尔(Thermo Fisher Scientific)科技公司
电热恒温水浴锅 Electro-thermostatic water bath	天津市泰斯特仪器有限公司
电热鼓风干燥箱 Electric blast drying oven	上海博讯医疗生物仪器股份有限公司
电子天平 Electronic balance	上海天美天平仪器有限公司

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The Relationship between Cat Periodontal Disease and Oral Flora and Its Prevention and Treatment

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