Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (7): 3528-3539.doi: 10.11843/j.issn.0366-6964.2025.07.044

• Clinical Veterinary Medicine • Previous Articles     Next Articles

Clinical Study of Novel Acellular Bioengineering Cornea Eccentric Deep Lamellar Keratoplasty for the Treatment of Feline Corneal Injury

XU Huihao1,2,*(), LI Qijuan1, DENG Hang1, DU Xinyue1, PENG Yunying1, YANG Heng1, ZHANG Dezhi1, CAO Lijing3, GAN Ling1, ZHENG Xiaobo1, YI Huashan1,*()   

  1. 1. College of Veterinary Medicine, Southwest University, Chongqing 402460, China
    2. Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing 400700, China
    3. Chongqing Rongchang Vocatiaonal Education Center, Chongqing 402460, China
  • Received:2024-08-12 Online:2025-07-23 Published:2025-07-25
  • Contact: XU Huihao, YI Huashan E-mail:xuhuihao2dai@163.com;dyxyihuashan@swu.edu.cn

Abstract:

The purpose of this study is to investigate the clinical therapeutic effect of a novel acellular bioengineering cornea (ABC) with eccentric lamellar keratoplasty on deep corneal injury in cats and the regulatory mechanism of related cytokines during the healing process. A corneal eccentric alkali burn model in 36 healthy adult local cats was established, and the model animals were randomly divided into three groups: ABC transplantation group (ABCTs group), corneal limbal conjunctival transplant group (CLCTs group), and control group. Evaluate the clinical efficacy and role of ABC eccentric deep lamellar keratoplasty through slit lamp examination, measurement of intraocular pressure and tear volume, corneal opacity and fluorescein staining score, optical coherence tomography (OCT) and histopathology. The corneal transparency of the ABCTs group was superior to other groups at all time-points, and the difference in corneal opacity score was extremely significant (P < 0.01). The intraocular pressure and tear volume of the three groups after surgery were within the normal physiological range, and the fluorescein staining scores showed an overall decreasing trend. However, the corneal re-epithelialization time in the ABCTs group was significantly earlier than that in the other groups (P < 0.01). OCT examination showed that the ABCTs group had earlier fusion of the graft with the host corneal stroma compared to the CLCTs group. There was no significant difference in corneal thickness between the ABCTs group and the normal cornea at 90 days after surgery (P>0.05), while the CLCTs group showed a significant decrease in corneal thickness (P < 0.01). Histological observation revealed that the ABCTs group had fewer inflammatory cells in corneal tissue compared to the CLCTs group, and the arrangement of corneal collagen was more regular. ABC transplantation can effectively treat cat eccentric corneal injury and provide excellent corneal transparency. ABC may promote corneal repair and transparency recovery by reducing collagen fibers and formation of scars.

Key words: feline, novel acellular bioengineering cornea, eccentric corneal injury, deep lamellar keratoplasty

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