猪卵母细胞玻璃化冷冻损伤的缓解策略

doi:10.11843/j.issn.0366-6964.2025.01.004

Abstract

Abstract:

Cryopreservation of oocytes is one of the important strategies for long-term storage of genetic resources in both humans and animal-assisted reproduction. However, due to the low surface-volume ratio and poor cell permeability, oocytes are more susceptible to cryoinjuries during vitrification, including ice crystals formation, osmotic shock, oxidative stress, and cryoprotectants toxicity, which seriously decreases their developmental ability after thawing. In contrast to other animals, porcine oocyte cryopreservation is more challenging owing to its abundant lipid droplets and poor hypothermia tolerance. Although live piglets have been produced from vitrified immature oocytes, the developmental competence of vitrified-oocytes remains to be further promoted compared with fresh oocytes. This paper mainly reviews the injury types, the mechanisms of damage, as well as the alleviating strategies of porcine vitrified-oocytes. It will provide further reference for establishing and improving the vitrification protocol of porcine oocytes.

Key words: porcine, oocytes, vitrification, cryoinjury

CLC Number:

S814.6

SUN Yawen, CHEN Siying, LI Kang, LENG Xuan, WANG Dong, PANG Yunwei. Strategies for Alleviating Cryoinjury of Porcine Vitrified-Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 36-44.

Figures/Tables 2

References 74

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缓解策略 Alleviating strategy	优点 Advantage	缺点 Disadvantage
增强渗透调节 Enhancement of osmotic regulation	平衡细胞内外渗透压，降低冷冻保护剂毒性	具有浓度依赖性，且物种之间的差异较大
抑制冰晶形成 Inhibition of ice crystals formation	抑制冰晶生长，降低溶液冰点	控冰材料价格相对昂贵，生物相容性较差
促进脂质重塑 Promotion of lipid remodeling	稳定细胞膜组成，维持细胞膜流动性	改变了卵母细胞固有的脂质结构特点，对后续发育的影响需进一步探索
降低氧化损伤 Reduction of oxidative damage	减少ROS生成和凋亡发生率	不同抗氧化剂的使用效果差异较大
工程化策略 Engineering strategy	仿生控冰，对细胞损伤较小	成本较高，可用范围局限

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Strategies for Alleviating Cryoinjury of Porcine Vitrified-Oocytes

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