Fig. 1

Lung sample collection points A-F in the figure indicate the sampling points of each lobe"

Table 1

Databases and version information"

Table 2

Primer sequences used for real-time PCR"

Fig. 2

Analysis of gene expression levels A. Box plot of gene expression level: blue for cattle, red for dzho, green for yak, horizontal axis is the sample name, vertical axis is log10 (FPKM+1), and the box plot of each region corresponds to 5 statistics (top to bottom, maximum, 3/4th quantile, median, 1/4th quartile and minimum). B. PCA diagram, the closer the sample clustering distance or PCA distance, the more similar the samples are. The horizontal and vertical coordinates PC1 and PC2 represent different principal components, and the percentages indicate the confidence with which the samples can be distinguished"

Fig. 3

Statistical histogram of differentially expressed genes The abscissa is the name of the group, the ordinate represents the number of differentially expressed genes, red is the up-regulated genes, and blue is the down-regulated genes"

Table 3

Statistical table of differential genes in the 3 groups"

Fig. 4

GO and KEGG-pathway analysis of differentially expressed genes between yak and cattle A. The horizontal axis represents the name of GO terms, and the vertical axis represents －log10(P). B. The horizontal axis is the Enrichment Score, indicating the degree of enrichment, the larger the value, the higher the enrichment; Large bubbles indicate entries that contain more differentially expressed protein-coding genes; The bubble colors change from purple to blue to green to red, with closer proximity to red indicating smaller enrichment P-values and greater significance"

Fig. 5

GO and KEGG-pathway analysis of differentially expressed genes between dzho and cattle A. The horizontal axis represents the name of GO terms, and the vertical axis represents －log10(P). B. The horizontal axis is the Enrichment Score, indicating the degree of enrichment, the larger the value, the higher the enrichment; Large bubbles indicate entries that contain more differentially expressed protein-coding genes; The bubble colors change from purple to blue to green to red, with closer proximity to red indicating smaller enrichment P-values and greater significance"

Fig. 6

GO and KEGG-pathway analysis of differentially expressed genes between yak and dzho A. The horizontal axis represents the name of GO terms, and the vertical axis represents －log10(P). B. The horizontal axis is the Enrichment Score, indicating the degree of enrichment, the larger the value, the higher the enrichment; Large bubbles indicate entries that contain more differentially expressed protein-coding genes; The bubble colors change from purple to blue to green to red, with closer proximity to red indicating smaller enrichment P-values and greater significance"

Fig. 7

Venn diagram of Yak vs. Cattle and Dzho vs. Cattle differential genes"

Fig. 8

GO and KEGG enrichment analysis of common differentially expressed genes A.The top 30 terms in the GO enrichment analysis: the horizontal axis is the －log10(P) and the vertical axis is the GO terms name.B.KEGG pathway enriched top 20 bubble map: the larger the bubble, the larger the number of genes encoding the differential proteins; the color of the bubble changes of violet-blue-green-red, and the smaller the enrichment P-value value, the greater the significance"

Fig. 9

Trend chart of significant modules In the module trend chart, the y-axis is the difference between each time node and the first time node, and the x-axis is the name of the time node, where the green thick line represents the trend of the module.In A, B figures, genes expressed in increasing amounts in the order of Cattle-Dzho-Yak; In C, D figures, genes are expressed in decreasing amounts in the order of Cattle-Dzho-Yak"

Fig. 10

GO and KEGG analysis of ascending genes"

Fig. 11

GO and KEGG analysis of decreasing genes"

Table 4

Genes related to hypoxia adaptation"

Fig. 12

Verification result map A-E. Sequencing results of different genes; F-J. Real-time fluorescence quantification results of different genes. The abscissa represents the sample name, and the ordinate represents the relative expression level of the genes"