北京黑猪肌内脂肪含量高、低组间脂质组差异分析

doi:10.11843/j.issn.0366-6964.2022.09.040

Abstract

Abstract: The purpose of this experiment was to reveal the relationship between the intramuscular fat content and lipidomics in Beijing black pig. In this experiment, 11 samples of high intramuscular fat group and 19 samples of low intramuscular fat group were selected from 400 210-day-old Beijing black pigs, and the average intramuscular fat content was 3.89% and 0.81%, respectively. For the selected individuals in high and low groups, 60 mg muscle samples were taken for lipid extraction, and finally non-targeted lipidomics detection was carried out. According to the test results, the two groups were analyzed by diversification, and the markers were screened by T test, difference multiple and partial least squares discriminant analysis. A total of 104 fatty acids, including 3 acylcarnitine (AcCa), 4 diglycerides (DG), 23 triglycerides (TG), 8 ardiolipin (CL), 21 phosphatidylcholine (PC), 29 phosphatidylethanolamine (PE), 3 phosphatidylglycerol (PG), 6 phosphatidylinositol (PI) and 7 phosphatidylserine (PS) were detected. The 33 kinds of lipids were preliminarily screened by T-test (P<0.05), of which TG were 23, AcCa were 2, DG were 4 and PE were 4. Further screening by VIP value (VIP>1) and FC value (FC>2 or FC<0.5),there were 22 kinds of lipids, including 21 TG and 1 DG. The results showed that lipidomics could be used as a method to distinguish the intramuscular fat content of Beijing black pig. The lipid difference substances affecting intramuscular fat content mainly concentrated in TG and DG.

Key words: Beijing black pig, lipidomics, triglyceride, intramuscular fat

CLC Number:

S828.2

ZHANG Run, LIU Hai, YANG Man, ZHANG Longchao, WANG Yuan. Analysis of Lipidome Difference between High and Low Intramuscular Fat Content Groups in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3262-3271.

References 40

[1]	零丽斯."猪"事变化如何影响农业产业?[J].中国农村科技, 2022(3):49-51.LING L S.How does the change of "pig" affect the agricultural industry?[J].China Rural Science & Technology, 2022(3):49-51.(in Chinese)
[2]	LI J, YANG Y Y, TANG C H, et al.Changes in lipids and aroma compounds in intramuscular fat from Hu sheep[J].Food Chem, 2022, 383:132611.
[3]	PUGLIESE C, SIRTORI F.Quality of meat and meat products produced from southern European pig breeds[J].Meat Sci, 2012, 90(3):511-518.
[4]	LIU H C, WEI W, LIN W M, et al.miR-32-5p regulates lipid accumulation in intramuscular fat of erhualian pigs by suppressing KLF3[J].Lipids, 2021, 56(3):279-287.
[5]	POKLUKAR K, ČANDEK-POTOKAR M, LUKAČ N B, et al.Lipid deposition and metabolism in local and modern pig breeds:A review[J].Animals (Basel), 2020, 10(3):424.
[6]	BAREA R, ISABEL B, NIETO R, et al.Evolution of the fatty acid profile of subcutaneous back-fat adipose tissue in growing Iberian and Landrace×Large White pigs[J].Animal, 2013, 7(4):688-698.
[7]	CABALLERO B, FINGLAS P M, TOLDRÁ F.Encyclopedia of food and health[M].Amsterdam:Elsevier Science, 2016:307-310.
[8]	KATSUMATA M.Promotion of intramuscular fat accumulation in porcine muscle by nutritional regulation[J].Anim Sci J, 2011, 82(1):17-25.
[9]	MALGWI I H, HALAS V, GRVNVALD P, et al.Genes related to fat metabolism in pigs and intramuscular fat content of pork:A focus on nutrigenetics and nutrigenomics[J].Animals (Basel), 2022, 12(2):150.
[10]	CASTELL A G, CLIPLEF R L, POSTE-FLYNN L M, et al.Performance, carcass and pork characteristics of castrates and gilts self-fed diets differing in protein content and lysine:Energy ratio[J].Can J Anim Sci, 1994, 74(3):519-528.
[11]	FERNANDEZ X, MONIN G, TALMANT A, et al.Influence of intramuscular fat content on the quality of pig meat-2.Consumer acceptability of m. Longissimus lumborum[J].Meat Sci, 1999, 53(1):67-72.
[12]	TU T, WU W D, TANG X Y, et al.Screening out important substances for distinguishing Chinese indigenous pork and hybrid pork and identifying different pork muscles by analyzing the fatty acid and nucleotide contents[J].Food Chem, 2021, 350:129219.
[13]	HILLGARTNER F B, SALATI L M, GOODRIDGE A G.Physiological and molecular mechanisms involved in nutritional regulation of fatty acid synthesis[J].Physiol Rev, 1995, 75(1):47-76.
[14]	KLOAREG M, NOBLET J, VAN MILGEN J.Deposition of dietary fatty acids, de novo synthesis and anatomical partitioning of fatty acids in finishing pigs[J].Br J Nutr, 2007, 97(1):35-44.
[15]	ZHANG Y F, ZHANG J J, GONG H F, et al.Genetic correlation of fatty acid composition with growth, carcass, fat deposition and meat quality traits based on GWAS data in six pig populations[J].Meat Sci, 2019, 150:47-55.
[16]	RESCONI V C, BUENO M, ESCUDERO A, et al.Ageing and retail display time in raw beef odour according to the degree of lipid oxidation[J].Food Chem, 2018, 242:288-300.
[17]	POLIDORI P, SANTINI G, KLIMANOVA Y, et al.Effects of ageing on donkey meat chemical composition, fatty acid profile and volatile compounds[J].Foods, 2022, 11(6):821.
[18]	WU N, WANG X C.Identification of important odorants derived from phosphatidylethanolamine species in steamed male Eriocheir sinensis hepatopancreas in model systems[J].Food Chem, 2019, 286:491-499.
[19]	BENET I, GUÀRDIA M D, IBAÑEZ C, et al.Low intramuscular fat (but high in PUFA) content in cooked cured pork ham decreased Maillard reaction volatiles and pleasing aroma attributes[J].Food Chem, 2016, 196:76-82.
[20]	MI S, SHANG K, JIA W, et al.Characterization and discrimination of Taihe black-boned silky fowl (Gallus gallus domesticus Brisson) muscles using LC/MS-based lipidomics[J].Food Res Int, 2018, 109:187-195.
[21]	ENGLMAIEROVÁ M, SKŘIVAN M, TAUBNER T, et al.Performance and meat quality of dual-purpose cockerels of dominant genotype reared on pasture[J].Animals (Basel), 2020, 10(3):387.
[22]	TANG H P, WANG X Y, XU L N, et al.Establishment of local searching methods for orbitrap-based high throughput metabolomics analysis[J].Talanta, 2016, 156-157:163-171.
[23]	LÍSA M, ŘEHULKOVÁ H, HANČOVÁ E, et al.Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts[J].J Chromatogr A, 2021, 1653:462380.
[24]	WELTE M A.Expanding roles for lipid droplets[J].Curr Biol, 2015, 25(11):R470-R481.
[25]	KAABIA Z, POIRIER J, MOUGHAIZEL M, et al.Plasma lipidomic analysis reveals strong similarities between lipid fingerprints in human, hamster and mouse compared to other animal species[J].Sci Rep, 2018, 8(1):15893.
[26]	LI J, TANG C H, ZHAO Q Y, et al. Integrated lipidomics and targeted metabolomics analyses reveal changes in flavor precursors in psoas major muscle of castrated lambs[J]. Food Chem, 2020, 333(4):127451.
[27]	TONAZZI A, GIANGREGORIO N, CONSOLE L, et al.The mitochondrial carnitine acyl-carnitine carrier (SLC25A20):molecular mechanisms of transport, role in redox sensing and interaction with drugs[J].Biomolecules, 2021, 11(4):521.
[28]	ZHOU L, ZHAO M J, BINDLER F, et al.Comparison of the volatiles formed by oxidation of phosphatidylcholine to triglyceride in model systems[J].J Agric Food Chem, 2014, 62(33):8295-8301.
[29]	WANG B, ZHAO X G, ZHANG B Y, et al.Assessment of components related to flavor and taste in Tan-lamb meat under different silage-feeding regimens using integrative metabolomics[J].Food Chem:X, 2022, 14:100269.
[30]	ZHANG C, LUO J Q, ZHENG P, et al.Differential expression of lipid metabolism-related genes and myosin heavy chain isoform genes in pig muscle tissue leading to different meat quality[J].Animal, 2015, 9(6):1073-1080.
[31]	FERNANDEZ X, MONIN G, TALMANT A, et al.Influence of intramuscular fat content on the quality of pig meat-1.Composition of the lipid fraction and sensory characteristics of m.Longissimus lumborum[J].Meat Sci, 1999, 53(1):59-65.
[32]	BRESSAN M C, BELO A T, AMARAL A, et al.The impact of genetic groups (Alentejano and F1 Landrace x Large White pigs) and body weight (90, 120 and 160 kg) on blood metabolites[J].Livest Sci, 2022, 255:104810.
[33]	CHEN J Y, CHEN F M, LIN X, et al.Effect of excessive or restrictive energy on growth performance, meat quality, and intramuscular fat deposition in finishing Ningxiang pigs[J].Animals (Basel), 2021, 11(1):27.
[34]	REN Y, HUANG J, YU X F, et al.Breed difference of porcine Sirtuin 1, adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL)[J].Livest Sci, 2013, 158(1-3):199-205.
[35]	FENG B, HUANG B, JING Y L, et al.Silymarin ameliorates the disordered glucose metabolism of mice with diet-induced obesity by activating the hepatic SIRT1 pathway[J].Cell Signal, 2021, 84:110023.
[36]	SHAN T, REN Y, LIU Y, et al.Breed difference and regulation of the porcine Sirtuin 1 by insulin[J].J Anim Sci, 2010, 88(12):3909-3917.
[37]	DALRYMPLE B P, GUO B.TRIENNIAL GROWTH AND DEVELOPMENT SYMPOSIUM:intramuscular fat deposition in ruminants and pigs:A transcriptomics perspective[J].J Anim Sci, 2017, 95(5):2272-2283.
[38]	HUANG Y Z, ZHOU L S, ZHANG J J, et al.A large-scale comparison of meat quality and intramuscular fatty acid composition among three Chinese indigenous pig breeds[J].Meat Sci, 2020, 168:108182.
[39]	LI J, ZHANG J Q, YANG Y Y, et al.Comparative characterization of lipids and volatile compounds of Beijing Heiliu and Laiwu Chinese black pork as markers[J].Food Res Int, 2021, 146:110433.
[40]	ZHAO S M, LI W Z, PAN H B, et al.Expression levels of candidate genes for intramuscular fat deposition in two Banna mini-pig inbred lines divergently selected for fatness traits[J].Genet Mol Biol, 2012, 35(4):783-789.

Analysis of Lipidome Difference between High and Low Intramuscular Fat Content Groups in Beijing Black Pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 40

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	SU Yanfang, YANG Man, NIU Naiqi, HOU Xinhua, ZHANG Longchao. Association Analysis of FABP3 and SCD Gene Polymorphisms with Meat Quality Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 966-975.
[4]	NIU Naiqi, SU Yanfang, YANG Man, HOU Xinhua, WANG Ligang, ZHANG Longchao. Association Analysis of Polymorphisms of HoxB Cluster Genes with Vertebral Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 113-121.
[5]	TIAN Weilong, LAN Ganqiu, ZHANG Longchao, WANG Lixian, LIANG Jing, LIU Xin. Detection of DKK3 and CCR1 Genes Polymorphisms and Their Association with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2083-2093.
[6]	NIU Naiqi, LIU Qian, HOU Xinhua, LIU Xin, WANG Ligang, ZHAO Fuping, GAO Hongmei, SHI Lijun, WANG Lixian, ZHANG Longchao. Association of Polymorphisms of NR6A1, VSX2, VRTN, LTBP2 Genes with Vertebral Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 2005-2014.
[7]	HOU Renda, ZHANG Run, NIU Naiqi, YANG Man, HUANG Xiaoyu, LI Huihui, ZHANG Longchao. Association of FUBP3 and USP43 Gene Polymorphisms with Loin Muscle Area in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4197-4206.
[8]	YANG Changheng, LI Qi, HUANG Wei, LIN Yaqiu, WANG Yong, XIANG Hua, ZHU Jiangjiang. Cloning of Goat DGAT1 Gene and Its Regulation on Lipid Deposition in Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 76-87.
[9]	LI Wufeng, SUN Yutong, GUAN Jiawei, ZHAO Jingwei, DU Min. Key Regulatory Factors of Intramuscular Fat Deposition in Donkey [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 364-375.
[10]	YUE Yongqi, HUA Yonglin, XIONG Yan, LIN Yaqiu, XIONG Xianrong, LI Jian. Research Progress of microRNA Regulation on the Fat Deposition of Subcutaneous Adipose Tissue and Intramuscular Fat in Animals [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2698-2709.
[11]	MA Qingshan, ZHANG Ruitao, WANG Changfa, LIU Guiqin, LI Yan. Research Progress on the Effects of Gut Microbiota and Its Functional Metabolites on Fat Metabolism and Intramuscular Fat Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 2921-2933.
[12]	REN Ling, HU Xin, XING Yishen, WANG Yahui, LI Junya, ZHANG Lupei. Effects of S100a10 on White and Brown Adipogenic Differentiation of Mice Preadipocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1171-1178.
[13]	WANG Yingming, XU Ya'ou, WANG Zhimin, XU Longyang, YANG Lei, LIN Yaqiu. Studies on the Cloning of KLF15 Gene, Tissue Expression Profile and the Association between Its Expression and Intramuscular Fat Content in Tibetan Chicken [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 261-270.
[14]	MA Jing, YAO Dawei, YANG Chunlei, LI Qiuling, WANG Tianzhen, CHEN Chengbin, SONG Wenqin, MA Yi. Effects of Interfering with CREB Gene on Lipid Synthesis Related Gene Expression and Triacylglycerol Synthesis in Mammary Epithelial Cells of Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2413-2421.
[15]	LI Kan, LIU Wen-zhong, ZHANG Rui-xin, LI Qian, ZHANG Ting, QIN Xu-ze, ZHANG Jian-xin, ZHAO Jun-xing. AMPK Regulates Sheep Muscle Derived Preadipocytes Differentiation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1594-1604.