首次从湖南猪源大肠杆菌中检出mcr-1阳性IncI2(Delta)质粒

doi:10.11843/j.issn.0366-6964.2024.11.043

Abstract

Abstract:

The rise of antibiotic resistance has brought renewed attention to older drugs like polymyxins, making it important to control the spread of resistance genes. In this study, we focused on colistin-resistant Escherichia coli strains from a pig farm in Hunan, China. Out of 240 samples, 172 E. coli strains were isolated and cultured, PCR was used to identify mcr-1 positive E. coli strains. These strains were tested by ten different antibiotics to check for their drug resistance, and whole-genome sequencing (WGS) was applied to analyze their genetic background. All the nine mcr-1 positive E. coli strains exhibit high levels of multidrug resistance. Four distinct MLST types (ST10, ST196, ST5229, ST46) and 4 serotypes (O83:H5, O16:H7, O16:H51, O9:H4) in the nine strains were identified. Further bioinformatics analyses showed that the ISApl1 element, which is commonly associated with mcr-1 transmission, was not observed in the present study, while the type Ⅳ secretion system genes were detected in all the nine mcr-1 positive E. coli strains. Plasmid conjugation experiments and the presence of polymorphic MLST haplotypes indicated a vertical plasmid-mediated mcr-1 transmission possibility. Notably, our study for the first time uncovered a pig origin IncI2(Delta) plasmid carrying mcr-1 gene in E. coli which is normally presented in the human clinical E. coli strains.

Key words: colistin resistance, mcr-1, plasmid IncI2(delta), multiple drug resistance, pig, Escherichia coli

CLC Number:

S852.612

Kangwei HUANG, Pengcheng ZHOU, Chenyu TIAN, Yi LAN, Zhiliang SUN. First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(Delta) Plasmids from Pigs in Hunan Province, China[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5278-5286.

Figures/Tables 5

Table 1

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Fig. 4

References 33

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菌株 Isolate	血清型 Serotype	来源 Origin	猪舍 Hog house	最小抑菌浓度MIC
菌株 Isolate	血清型 Serotype	来源 Origin	猪舍 Hog house	CTX(2)	CAZ(4)	MEM(8)	GEN(2)	CIP(0.5)	AMK(8)	CL(2)	TCY(4)^*	FLR(16)^*	SXT(4)
23cg187	O83:H5	NP	NH 1	＞32	＞32	＞8	＞64	＞2	＞64	＞16	＞8	8	8
23cg189	O16:H7	NP	NH 1	＞32	＞32	4	＞64	2	＞64	＞16	8	2	4
23cg201	O16:H7	NP	NH 4	＞32	＞32	4	＞64	＞2	＞64	＞16	＞8	8	8
23cg214	O16:H51	FP	FH 2	＞32	＞32	0.25	＞64	＞2	＞64	＞16	8	4	4
23cg215	O16:H51	FP	FH 2	＞32	＞32	4	＞64	＞2	＞64	＞16	＞8	4	4
23cg223	O9:H4	FP	FH 6	＞32	32	1	＞64	＞2	＞64	＞16	＞8	2	2
23cg225	O16:H51	FP	FH 6	＞32	＞32	4	＞64	＞2	＞64	＞16	＞8	4	4
23cg226	O16:H51	FP	FH 6	＞32	＞32	8	＞64	＞2	＞64	＞16	＞8	4	4
23cg230	O9:H4	FP	FH 6	＞32	＞32	8	＞64	＞2	＞64	＞16	＞8	16	16

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First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(Delta) Plasmids from Pigs in Hunan Province, China

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