Hlcs干扰对C2C12细胞成肌成脂分化后糖酵解基因表达的影响

doi:10.11843/j.issn.0366-6964.2022.10.012

Abstract

Abstract: The aim of this study was to investigate the effect of holocarboxylase synthetase (Hlcs) on glycolytic gene expression and to lay the foundation for further studies on cellular glycolysis. In this experiment, C2C12 cells were used as the experimental material, and siRNA technology was used to interfere with the Hlcs gene, and the effect of Hlcs gene interference on the glycolysis gene expression was detected by fluorescence quantitative PCR and Western blotting. The results showed that, during the differentiation of C2C12 cells, the expression trends of Hlcs genes was consistent with Pfkm, Pkm, Hk-2 genes, suggesting that Hlcs genes were correlated with glycolysis. After interfering with Hlcs, the number of myotubes formed during cell differentiation was significantly less than that in the control group, the mRNA level of the glycolytic rate-limiting enzyme Pfkm was significantly decreased (P<0.05), and the mRNA and protein levels of the Pkm were significantly increased (P<0.05). As a coenzyme of carboxylase, biotin could significantly up-regulate the mRNA levels of key enzymes Pkm and Hk-2 (P<0.05). After interfering with Hlcs, the number of lipid droplets formed by cell differentiation was obviously less than that in the control group, the mRNA level of Pfkm was significantly increased (P<0.05), and the mRNA level and protein expression level of the Pkm were significantly increased (P<0.05); The addition of biotin could significantly up-regulate the mRNA level of Pfkm and Pkm (P<0.05). In summary, during the differentiation of C2C12 cells, Hlcs genes were expressed in the same trend as glycolytic genes, and interfering with Hlcs genes could inhibit the expression of Pfkm and Hk-2 genes and promote the expression of Pkm in myogenic differentiated C2C12 cells; while promoting the expression of Pfkm and Pkm and inhibiting the expression of Hk-2 genes in lipogenic differentiated C2C12 cells.

Key words: Hlcs gene, siRNA interference, C2C12 cells, myogenic and adipogenic induction, glycolysis genes

CLC Number:

S813

DAI Yuxing,SHI Yinyin,WEN Zuochen,LUO Yunyan,ZHU Xueli,ZHENG Chunting,LI Shuying,HONG Liang,ZHANG Jianbin,GUO Liang,PU Lei. The Effect of Hlcs Interference on Glycolytic Gene Expression in C2C12 Cells after Myogenic Lipogenic Differentiation[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3391-3402.

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The Effect of Hlcs Interference on Glycolytic Gene Expression in C2C12 Cells after Myogenic Lipogenic Differentiation

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