SPF大白猪和长白猪CIITA基因剪接突变体的鉴定分析及在不同组织中的表达模式

doi:10.11843/j.issn.0366-6964.2021.08.006

Abstract

Abstract: The study aimed to identify and analyze CIITA splicing mutants in SPF Yorkshire and Landrace pigs and study their expression patterns in different pig tissues. In this study, the CIITA gene was amplified using specific primers, cloned and sequenced in 8 SPF Yorkshire and 7 SPF Landrace pigs aged 5 weeks, and the splicing mutant characteristics of the obtained sequences were analyzed. The online software was used for bioinformatics analysis of different splicing mutants. The expression patterns of CIITA splicing mutants in 8 tissues of 2 SPF Landrace pigs were detected by fluorescence quantitative PCR method. The results showed that 3 different CIITA splicing mutants (CIITA-A, CIITA-B and CIITA-C) in SPF Yorkshire and Landrace pigs were identified with CDS lengths of 939, 984 and 1 698 bp, respectively. The deletion of partial nucleotides (40 and 199 bp) in Exon 11 in CIITA-A and CIITA-B mutants made the termination codon appeared in advance, which resulted in the splicing of the domain encoding CIITA protein and the deletion of 4 leucine rich repeat domains, the structure of the encoded protein had changed obviously. The results of phylogenetic tree showed that pigs had close relationship with sheep and cattle, and had higher conservation in different pig breeds for CIITA. Moreover, different splicing mutants of CIITA were expressed in 8 pig tissues, and the expression levels tended to be consistent, with high expression levels in spleen or lung, followed by kidney, spinal cord, thyroid, liver and thymus, and the lowest expression level in heart. A total of 3 CIITA splicing mutants were obtained in SPF Yorkshire and Landrace pigs, two of which deleted 4 leucine rich repeat domains. Different splicing mutants were expressed in different pig tissues with slightly different levels. The results of this study lay a theoretical foundation for the further studies on the immune regulation function of CIITA gene in the host.

Key words: SPF pig, CIITA gene, splicing mutants, expression

CLC Number:

S828.2

MA Lina, LIU Yinghua, WANG Youqi, GAO Caixia, CHEN Hongyan. Identification and Analysis of CIITA Gene Splicing Mutants in SPF Yorkshire and Landrace Pigs and Their Expression Patterns in Different Tissues[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2124-2137.

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Identification and Analysis of CIITA Gene Splicing Mutants in SPF Yorkshire and Landrace Pigs and Their Expression Patterns in Different Tissues

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