牦牛IGFBP4和IGFBP5基因的克隆及其在各组织和不同生长阶段肝中的差异表达分析

doi:10.11843/j.issn.0366-6964.2021.07.009

Abstract

Abstract: This study aimed to clone IGFBP4 and IGFBP5 genes of yak, and detect their expression level in different tissues and liver at different growth stages of yak. In this study, 9 healthy female Maiwa yaks with triplicates were selected from 1 day old, 15 months old and 5 years old with body weight of (12.35±1.85), (98.88±2.50) and (268.55±27.82) kg, respectively. The heart, liver, spleen, lung, kidney and intestine of 5 years old yak and the liver of yak at 3 growth ages were collected. The IGFBP4 and IGFBP5 genes were cloned and their sequences were analyzed by bioinformatics method. In addition, the expressions of IGFBP4 and IGFBP5 were detected by qRT-PCR, Western blot and immunohistochemistry in 6 tissues and liver at 3 growth stages of yak. The results indicated that the CDS sequence of IGFBP4 and IGFBP5 genes were 777 and 816 bp (GenBank accession number:MT012934 and MT003005), encoding a total of 258 and 271 amino acids, respectively. The homology of IGFBP4 and IGFBP5 genes of yak compared with the cattle reached 99.6% and 99.5%, respectively. At the same time, compared with the kidney, heart, intestine, spleen and lung, the expression of IGFBP4 and IGFBP5 genes were the highest in the liver of 5 years old yak (P<0.01). The expression of IGFBP4 gene were significantly higher than that of IGFBP5 gene in these tissues (P<0.01). With the increase of age, the mRNA and protein expression of IGFBP4 and IGFBP5 in yak liver were showed an upward trend in the liver of yak, which was the expression of 5 years old >15 months old >1 day old. Compared with 1 day old and 15 months old, the expression level of IGFBP4 and IGFBP5 in yak liver were the highest in 5 years old (P<0.01). Moreover, the expression of IGFBP4 was extremely significant higher than that of IGFBP5(P<0.01). The results suggest that IGFBP4 and IGFBP5 may co-regulate liver growth and development of yak. It will benefit further study of the role and expression regulation of IGFBP4 and IGFBP5 in the growth and development of yak liver.

Key words: IGFBP4, IGFBP5, yak, cloning and expression, liver

CLC Number:

S823.85

FU Fang, WANG Li, LUO Xiaolin, GUAN Jiuqiang. Cloning of Yak IGFBP4 and IGFBP5 and Their Expression Patterns in Different Tissues and Liver at Different Growth Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 1858-1868.

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Cloning of Yak IGFBP4 and IGFBP5 and Their Expression Patterns in Different Tissues and Liver at Different Growth Stages

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