硝酸钠和延胡索酸二钠混合物对水牛瘤胃甲烷含量、体外发酵参数、脂肪酸组成和瘤胃微生物数量的影响

doi:10.11843/j.issn.0366-6964.2020.10.029

Abstract

Abstract: The objective of this study was to investigate the effects of different sodium nitrate and disodium fumarate ratios on ruminal methane production, fermentation parameters, fatty acids profiles and microbial population in water buffalo in vitro in presence of α-linolenic acid. Three female water buffaloes (body weight of (650±50) kg) with permanent rumen fistula were selected as the donors of rumen contents. Treatments additives were prepared as sodium nitrate and disodium fumarate mixtures at ratios of 2:1, 1:1, 1:2. Control group without any sodium nitrate or disodium fumarate. All groups were added with 0.25 mg·mL^-1 α-linolenic acid. The concentration of sodium nitrate and disodium fumarate mixtures was 1 mg·mL^-1. The results showed that mehtane production were reduced with different ratios of sodium nitrate and disodium fumarate mixture, the average decrease was 90.63%. The total volatile fatty acid (TVFA) concentration and most rumen microorganisms population were significantly reduced (P<0.05), but the concentration of acetate, EPA and CLA, ratios of acetate to propionate ratio and UFA/SFA were significantly increased (P<0.05) with sodium nitrate and disodium fumarate mixtures at ratio of 2:1. 3) Sodium nitrate and disodium fumarate mixtures at ratio of 1:2 had no significant effect on TVFA content, fatty acid content and rumen microbial populaiton(P>0.05). 4) The concentrations of EPA, CLA and UFA/SFA ratio were increased (P<0.05) with sodium nitrate and disodium fumarate mixtures at ratio of 1:1, but Butyrivibrio fibrisolvens and atypical butyrivibrio populations were decreased. It can be concluded that different proportion of sodium nitrate and sodium fumarate mixture can cause methane reduction, the adverse effect of sodium nitrate on rumen fermentation can be alleviated by the increased proportion of disodium fumarate.

Key words: sodium nitrate, disodium fumarate, methane, in vitro fermentation, rumen microorganism

CLC Number:

S823.8⁺3

GUO Yanxia, LI Mengwei, TANG Zhenhua, PENG Lijuan, PENG Kaiping, LIANG Xin, XIE Fang, YANG Chengjian. Effects of Sodium Nitrate and Disodium Fumarate Mixture on Ruminal Methane Production, Fermentation Parameters, Fatty Acids Profiles and Rumen Microbial Population in Water Buffalo In vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2599-2608.

References 39

[1]	王荣,王敏,邓近平,等.硝酸钠对奶牛瘤胃氢气、发酵模式和甲烷排放量的影响[C]//中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集.武汉:中国畜牧兽医学会动物营养学分会,2016:1.WANG R,WANG M,DENG J P,et al.Effects of sodium nitrate on rumen hydrogen,fermentation mode and methane emission of dairy cows[C]//Proceedings of the 12th Animal Nutrition Academic Seminar of Animal Nutrition Branch of China Society of Animal Husbandry and Veterinary Medicine.Wuhan:Animal Nutrition Branch of China Society of Animal Husbandry and Veterinary Medicine,2016:1.(in Chinese)
[2]	ENGEL C A R,STRAATHOF A J J,ZIJLMANS T W,et al.Fumaric acid production by fermentation[J].Appl Microbiol Biotechnol,2008,78(3):379-389.
[3]	周亚文,张玉杰,林波,等.瘤胃甲烷生成过程中微生物之间的相互关系[J].动物营养学报,2011,23(4):556-562.ZHOU Y W,ZHANG Y J,LIN B,et al.Microbial interactions in ruminal methanogenesis[J].Chinese Journal of Animal Nutrition,2011,23(4):556-562.(in Chinese)
[4]	YU C W,CHEN Y S,CHENG Y H,et al.Effects of fumarate on ruminal ammonia accumulation and fiber digestion in vitro and nutrient utilization in dairy does[J].J Dairy Sci,2010,93(2):701-710.
[5]	BEAUCHEMIN K A,MCGINN S M.Methane emissions from beef cattle:effects of fumaric acid,essential oil,and canola oil[J].J Anim Sci,2006,84(6):1489-1496.
[6]	IWAMOTO M,ASANUMA N,HINO T,et al.Effects of nitrate combined with fumarate on methanogenesis,fermentation,and cellulose digestion by mixed ruminal microbes in vitro[J].Nihon Chikusan Gakkaiho,1999,70(6):471-478.
[7]	姜宇晨.硝酸钾和延胡索酸二钠联合使用对绵羊瘤胃发酵、甲烷生成和免疫功能的影响[D].呼和浩特:内蒙古农业大学,2019.JIANG Y C.Effects of combination of potassium nitrate and disodium fumarate on rumen fermentation,methane production and immune function in sheep[D].Hohhot:Inner Mongolia Agricultural University,2019.(in Chinese)
[8]	MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J].J Agric Sci,1979,93(1):217-222.
[9]	ZHOU Z M,MENG Q X,YU Z T.Effects of methanogenic inhibitors on methane production and abundances of methanogens and cellulolytic bacteria in in vitro ruminal cultures[J].Appl Environ Microbiol,2011,77(8):2634-2639.
[10]	胡伟莲,王佳堃,吕建敏,等.瘤胃体外发酵产物中的甲烷和有机酸含量的快速测定[J].浙江大学学报:农业与生命科学版,2006,32(2):217-221.HU W L,WANG J K,LÜ J M,et al.Rapid gas chromatogram determination of methane,organic acid in in vitro ruminal fermentation products[J].Journal of Zhejiang University:Agriculture & Life Sciences,2006,32(2):217-221.(in Chinese)
[11]	鲁秋英.日粮淀粉/FNDF比例对高产奶牛瘤胃液、血液、乳液和粪便相关代谢指标影响的调查研究[D].呼和浩特:内蒙古农业大学,2014.LU Q Y.Effects of different ratios of dietary starch to neutral detergent fiber on Rumen blood,milk and feces metabolism status in high producing lactating cows[D].Hohhot:Inner Mongolia Agricultural University,2014.(in Chinese)
[12]	LI X Z,YAN C G,CHOI S H,et al.Effects of addition level and chemical type of propionate precursors in dicarboxylic acid pathway on fermentation characteristics and methane production by rumen microbes in vitro[J].Asian-Australas J Anim Sci,2009,22(1):82-89.
[13]	XU T L,TAO H,CHANG G J,et al.Lipopolysaccharide derived from the rumen down-regulates stearoyl-CoA desaturase 1 expression and alters fatty acid composition in the liver of dairy cows fed a high-concentrate diet[J].BMC Vet Res,2015,11(1):52.
[14]	DENMAN S E,MCSWEENEY C S.Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen[J].FEMS Microbiol Ecol,2006,58(3):572-582.
[15]	SHINGFIELD K J,KAIRENIUS P,ÄRÖLÄ A,et al.Dietary fish oil supplements modify ruminal biohydrogenation,alter the flow of fatty acids at the omasum,and induce changes in the ruminal Butyrivibrio population in lactating cows[J].J Nutr,2012,142(8):1437-1448.
[16]	ZOETENDAL E G,AKKERMANS A D L,DE VOS W M.Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria[J].Appl Environ Microbiol,1998,64(10):3854-3859.
[17]	DEMAN S E,TOMKINS N W,DEMAN S E,et al.Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane[J].FEMS Microbiol Ecol,2007,62(3):313-322.
[18]	STEVENSON D M,WEIMER P J.Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR[J].Appl Microbiol Biotechnol,2007,75(1):165-174.
[19]	刘丽英.饲草型全混日粮的评价及配方筛选[D].呼和浩特:内蒙古农业大学,2011.LIU L Y.Evaluation and formulation screening of forage type total mixed rations[D].Hohhot:Inner Mongolia Agricultural University,2011.(in Chinese)
[20]	PATRA A K,YU Z T.Combinations of nitrate,saponin,and sulfate additively reduce methane production by rumen cultures in vitro while not adversely affecting feed digestion,fermentation or microbial communities[J].Bioresour Technol,2014,155:129-135.
[21]	PATRA A K,YU Z T.Effective reduction of enteric methane production by a combination of nitrate and saponin without adverse effect on feed degradability,fermentation,or bacterial and archaeal communities of the rumen[J].Bioresour Technol,2013,148:352-360.
[22]	NGUYEN S H,LI L,HEGARTY R S.Effects of rumen protozoa of Brahman heifers and nitrate on fermentation and in vitro methane production[J].Asian-Australas J Anim Sci,2016,29(6):807-813.
[23]	ZHOU Z M,YU Z T,MENG Q X.Effects of nitrate on methane production,fermentation,and microbial populations in in vitro ruminal cultures[J].Bioresour Technol,2012,103(1):173-179.
[24]	毛胜勇,王新峰,朱伟云.体外法研究延胡索酸二钠对瘤胃微生物发酵活力及甲烷产量的影响[J].草业学报,2010,19(2):69-75.MAO S Y,WANG X F,ZHU W Y.Effects of disodium fumarate on in vitro methane production and fermentation of rumen microbial[J].Acta Prataculturae Sinica,2010,19(2):69-75.(in Chinese)
[25]	刘丽慧,刘宝龙,崔宏晓,等.梯度添加硝酸盐或硫酸盐对瘤胃体外发酵和总菌数量的影响[J].家畜生态学报,2017,38(8):29-34.LIU L H,LIU B L,CUI H X,et al.Effects of gradient addition of nitrate and sulfate on rumen fermentation and total bacteria count in vitro[J].Acta Ecologiae Animalis Domastici,2017,38(8):29-34.(in Chinese)
[26]	孙雨坤,闫晓刚,班志彬,等.硝酸盐对肉牛甲烷产量和生长性能的影响[J].中国农业大学学报,2017,22(7):54-60.SUN Y K,YAN X G,BAN Z B,et al.Effect of nitrate on the methane production and productivity of cattle[J].Journal of China Agricultural University,2017,22(7):54-60.(in Chinese)
[27]	赖瀚卿,刘云芳,雷晓萍,等.延胡索酸二钠对断奶羔羊瘤胃发育及瘤胃发酵功能的影响[J].畜牧与兽医,2016,48(2):36-40.LAI H Q,LIU Y F,LEI X P,et al.Effects of dietary disodium fumarate on rumen development and rumen fermentation function in weaning lambs[J].Animal Husbandry & Veterinary Medicine,2016,48(2):36-40.(in Chinese)
[28]	JIN W,XUE C X,LIU J H,et al.Effects of disodium fumarate on in vitro rumen fermentation,the production of lipopolysaccharide and biogenic amines,and the rumen bacterial community[J].Curr Microbiol,2017,74(11):1337-1342.
[29]	赵丽萍.硝酸盐对肉牛瘤胃发酵、微生物多样性、血液生化及抗氧化性能的影响[D].北京:中国农业大学,2015.ZHAO L P.Effects of nitrate on rumen fermentation,microbe diversity,blood biochemical and antioxidative ability[D].Beijing:China Agricultural University,2015.(in Chinese)
[30]	GUYADER J,TAVENDALE M,MARTIN C,et al.Dose-response effect of nitrate on hydrogen distribution between rumen fermentation end products:an in vitro approach[J].Anim Prod Sci,2016,56(3):224-230.
[31]	KEPLER C R,HIRONS K P,MCNEILL J J,et al.Intermediates and products of the biohydrogenation of linoleic acid by Butyrinvibrio fibrisolvens[J].J Biol Chem,1966,241(6):1350-1354.
[32]	VAN DE VOSSENBERG J L C M,JOBLIN K N.Biohydrogenation of C18 unsaturated fatty acids to stearic acid by a strain of Butyrivibrio hungatei from the bovine rumen[J].Lett Appl Microbiol,2003,37(5):424-428.
[33]	LI D,WANG J Q,BU D P.Ruminal microbe of biohydrogenation of trans-vaccenic acid to stearic acid in vitro[J].BMC Res Notes,2012,5(1):97.
[34]	YANG C J,MCKAIN N,MCCARTNEY C A,et al.Consequences of inhibiting methanogenesis on the biohydrogenation of fatty acids in bovine ruminal digesta[J].Anim Feed Sci Technol,2019,254:114189.
[35]	HALMEMIES-BEAUCHET-FILLEAU A,KAIRENIUS P,AHVENJRVI S,et al.Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio[J].J Dairy Sci,2013,96(4):2428-2447.
[36]	MBIRIRI D T,CHO S,MAMVURA C I,et al.Effects of a blend of garlic oil,nitrate and fumarate on in vitro ruminal fermentation and microbial population[J].J Anim Physiol Anim Nutr (Berl),2017,101(4):713-722.
[37]	ARIF M,SARWAR M,HAYAT Z,et al.Effect of supplementary sodium nitrate and sulphur on methane production and growth rates in sheep and goats fed forage based diet low in true protein[J].J Anim Plant Sci,2016,26(1):69-78.
[38]	LATHAM E A,ANDERSON R C,PINCHAK W E,et al.Insights on alterations to the rumen ecosystem by nitrate and nitrocompounds[J].Front Microbiol,2016,7:228.
[39]	王荣,谭利伟,王敏,等.硝酸钠和2-溴乙烷磺酸钠对山羊体外瘤胃发酵甲烷、氢气和挥发性脂肪酸生成的影响[J].动物营养学报,2015,27(5):1586-1595.WANG R,TAN L W,WANG M,et al.Effect of sodium nitrate and 2-bromoethanesulphonate on Methane,hydrogen and volatile fatty acids production of in vitro ruminal fermentation[J].Chinese Journal of Animal Nutrition,2015,27(5):1586-1595.(in Chinese)

Effects of Sodium Nitrate and Disodium Fumarate Mixture on Ruminal Methane Production, Fermentation Parameters, Fatty Acids Profiles and Rumen Microbial Population in Water Buffalo In vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 39

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	HU Liping, SHEN Ziliang, WANG Quan, YU Zitong, ZHANG Qiqi, MAO Yongjiang, YANG Zhangping, ZHANG Huimin. Changes of Rumen Microbe and Milk Fatty Acid Composition during Early Lactation [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3018-3028.
[2]	YU Yongtao, MAO Yanni, ZHAO Qingmei, LI Jinrong, BAI Xiaonan, XUE Jiaqi, ZHANG Haodong. Expression Pattern Analysis of Related Genes in SWN Gene Cluster of Alternaria oxytropis Mutated by Ethyl Methylate [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1241-1251.
[3]	LIU Zhihao, JIA Peng, LAI Qi, DONG Lifeng, WU Qiujue, GAO Yanhua, TIAN Zhonghong, DIAO Qiyu. Characteristics of Rumen Fermentation and Methane Production in Different Parity Dry Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4296-4305.
[4]	ZHANG Ruixue, LIU Xin, XU Xiaofeng, ZHANG Bo, TANG Yulin, REN Man, GUO Yansheng. Study on the Changes of Rumen Metabolites and Metabolic Pathways in Dairy Cows before and after Parturition [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3137-3148.
[5]	SUN Xueli, LI Qiufeng, CAO Yufeng, YAN Jinling, LIU Taotao, ZHANG Yixian, LI Jianguo, GAO Yanxia, LI Shujing, XIE Peng, ZHANG Xiujiang. Effects of Dietary Addition of N-carbamylglutamate on Growth Performance, Rumen Fermentation， Rumen Microflora and Methane Emission of Holstein Bulls [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1903-1912.
[6]	WU Wancheng, MA Tao, LIU Na, YANG Lei, CHEN Guoshun, DIAO Qiyu. Effect of Resveratrol on Methane Production, Nutrient Degradation and Microbial Community under Different Substrates [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1281-1294.
[7]	ZHAO Jiaqi, WEN Yongli, AN Yajing, LI Ziqian, QI Peisen, LI Qiang, HOU Dingchao. Response of Antibiotic Resistance Genes (ARGs) in Rumen of Yak to Three Exogenous Stimulating Factors [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1126-1137.
[8]	ZHOU Yan, DIAO Qi-yu, DONG Li-feng, DENG Kai-dong, XU Gui-shan, MA Tao. The Effect of Different Ratios of Dietary Non-fibrous Carbohydrate (NFC) to Neutral Detergent Fiber (NDF) on Production Performance, Nutrient Digestibility and Methane Emissions of Dorper×Thin-tailed Han Crossbred Growing Ewes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1405-1415.
[9]	MAO Hong-xiang, GAO Feng-xian, WANG Min, JIANG Zai-yang, ZHANG Xiu-min, LONG Dong-lei, WANG Rong, TAN Zhi-liang. Evaluating the Accuracy of Models to Predict Enteric Methane Emissions in Beef Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 690-698.
[10]	XIE Tian-yu，WANG Min，WANG Rong，YAN Zhi-cheng，SHI Hui-yu，GAO Min，TAN Zhi-liang. Evaluation of Accuracy of Models to Predict Enteric Methane Emissions in Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1574-1583.
[11]	LI Qiufeng;CAO Yufeng;LI Jianguo. comEffects of Different Rumen Degradable Protein Balance (RDPB) on Rumen Fermentation, Total Gas and Methane Production in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(4): 572-579.
[12]	LIU Wei;XIN Hangshu;LIU Caijuan;WEN Qinan;TAN Weizhuo;ZHANG Yonggen . Effects of Hainanmycin on Ruminal Fermentation Pattern, Methane Production and Micro Flora [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(2): 242-249.
[13]	SHEN Shasha;YANG Hongjian;ZHANG Xiaoming. Advance in Classification and Enzymological Characteristics of Microbial Phytase in the Rumen [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(12): 1655-1660.
[14]	ZHANG Yuan-qing;WEI Ji-an;MENG Qing-xiang. The Fermentable Characterization of Different Source Plant Cell Walls and Their Contribution to the Methane Emission in Ruminant Animals in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(10): 992-998.