翠雀内生真菌分离鉴定及分布特性研究

doi:10.11843/j.issn.0366-6964.2020.09.028

Abstract

Abstract: In order to explore the species of endophytic fungi from a poisonous plant, Delphinium grandiflorum L. In this experiment, surface disinfection was used for separation, morphological identification was used, and phylogenetic tree was constructed using ITS sequence to determine the species of endophytic fungi. The results showed that 22 species of endophytic fungi were isolated from D. grandiflorum L., they were mainly divided into two groups according to their phylogenetic relationship, belonging to 6 classes, 6 orders, 6 families, and 7 genera. Among them, Alternaria sp. was the dominant species (accounting for 31.82% of the isolated strains, mainly distributed in flowers and leaves), endophytic fungi are most commonly distributed in flowers (accounting for 40.91% of the isolates), followed by leaves (31.82%), roots (22.73%) and stems (4.55%). The results indicated that the distribution of endophytic fungi in D. grandiflorum L. is different in different tissues.

Key words: Delphinium grandiflorum L., endophytic fungus, isolation and identification, distribution characteristics, Alternaria sp.

CLC Number:

S856.9
S452

ZHU Yanli, LI Bo, ZHANG Yu, SUN Lu, HUANG Enxia, MO Chonghui, ZHAO Baoyu, LU Hao. Isolation, Identification and Distribution Characteristics of Fungal Endophyte in Delphinium grandiflorum L.[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2302-2311.

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Isolation, Identification and Distribution Characteristics of Fungal Endophyte in Delphinium grandiflorum L.

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