牦牛AIF-1蛋白表达及对巨噬细胞炎性因子mRNA的影响

doi:10.11843/j.issn.0366-6964.2020.05.028

Abstract

Abstract: The objective of this study was to express the allograft inflammatory factor-1 (AIF-1) of yak in E.coli BL21(DE3) and investigate its effect on mouse macrophages. The expression levels of AIF-1 gene in 5 tissues of yak were detected by q-PCR. A prokaryotic expression vector was constructed to express and purify AIF-1 protein. The expression levels of 4 inflammatory factors in mouse macrophages were detected by q-PCR. The results showed that the expression level of AIF-1 gene was the highest in the spleen of Maiwa yak, which was extremely significantly higher than those in other tissues (P<0.01). Recombinant protein of approximately 29.47 ku was expressed and purified. The expression levels of IL-1β, IL-6, TNF-α and iNOS in mouse macrophages were promoted by 1.0, 10.0 and 100.0 μg·mL^-1 of AIF-1 protein. The results indicated that AIF-1 played a role in the immune response of macrophages. This article will be benefit for further study on the function of yak AIF-1.

Key words: allograft inflammatory factor-1, prokaryotic expression, macrophage, inflammatory factor

CLC Number:

S823.85

LI Juan, WANG Li, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHANG Xiangfei. Expression of Yak AIF-1 Protein and Its Effect on mRNA of Inflammatory Factors in Macrophagocyte[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1163-1166.

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Expression of Yak AIF-1 Protein and Its Effect on mRNA of Inflammatory Factors in Macrophagocyte

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