一株塞内卡病毒A全基因组序列测定与致病性分析

doi:10.11843/j.issn.0366-6964.2020.04.018

Abstract

Abstract: The aim of this study was to clarify genomic characteristics, homology, and pathogenicity of Senecavirus A (strain FJLY). Strain SVA-FJLY isolated from a farm in Fujian Province was used as focus of study. Five pairs of primers were designed based on whole-genome sequence of SVV-001 (GenBank No. DQ641257.1) and whole-genome sequence of strain SVA-FJLY was obtained and analyzed by RT-PCR, sequencing, and splicing. Piglets aged 3-4 weeks old were also infected with this strain by intranasal spray. Results showed that genome of strain SVA-FJLY had a full length of 7 275 bp (excluding PolyA). It encoded a polyprotein, VP1 protein, and VP2 protein. Strain SVA-FJLY had high homology with reference strain and HeB01-2017 strain (MF967574.1), with highest homology reaching 98.5%, while it had low homology with SVA prototype strain SVV-001 (DQ641257.1), reaching 93.9%. Strain SVA-FJLY belonged to the same branch as several Senecavirus A strains isolated in China, and domestically had closest relationship with strain HeB01-2017, while regarding foreign strains it had closest relationship with Colombia-2016 (KX857728.1). Animal experiments showed that two of three animals in experimental group suffered from the disease, while all three animals in control group remained normal. All six piglets survived 10 days after challenge of experimental group. Whole-genome sequence of strain SVA-FJLY was obtained by genome sequencing. Genomic characteristics of strain SVA-FJLY and its homology with other strains were clarified. Pathogenicity of strain SVA-FJLY was also revealed. Moreover, a reference for reverse genetics and vaccine development for SVA was provided, and database with genome information on SVA was also enriched.

Key words: Senecavirus A, RT-PCR amplification, sequence analysis, genetic evolution tree, homology, strain FJLY, pathogenicity

CLC Number:

S852.659.6

LAI Longyong, LIU Xiaolong, TAN Lining, YE Shengcong, QIU Lingshan, CHEN Shengyong, LIU Chuchu, XU Lei. Genome Sequencing and Pathogenicity Analysis of Senecavirus A, SVA-FJLY[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 820-826.

References 26

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Genome Sequencing and Pathogenicity Analysis of Senecavirus A, SVA-FJLY

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