miR-142和miR-144靶向FoxO1基因调节绵羊前体脂肪细胞分化的研究

doi:10.11843/j.issn.0366-6964.2018.04.003

Abstract

Abstract:

This study aimed to predict and validate the crucial miRNAs that regulate FoxO1 gene expression, and to elucidate the functional mechanism of these miRNAs in ovine adipose differentiation.Four online softwares were used to predict the miRNAs that might target FoxO1. A dual-luciferase reporter assay system was employed to detect luciferase activity. qPCR, Western blotting and Oil red O staining were used to detect the effect of these miRNAs on the expression of FoxO1 both at mRNA and protein levels, and on the regulation of ovine preadipocyte differentiation. The results showed that FoxO1 was targeted by both miR-142 and miR-144.Overexpression of miR-142 or miR-144 significantly inhibited the luciferase activity of the dual-luciferase reporter vector, and reduced the expression of FoxO1 mRNA (P<0.01) and protein (P<0.05). Both miR-142 and miR-144 promoted the differentiation of ovine preadipocytes and accelerated the formation of lipid droplets. In conclusion, miR-142 and miR-144 can down-regulate the expression of FoxO1, and further down-regulate PPARγ expression,thus promote the differentiation of ovine preadipocytes.

CLC Number:

S826.2

ZHAO Yan-yan, QIAO Li-ying, JING Jiong-jie, PAN Yang-yang, REN Duan-yang, WANG Shu-fang, Lü Xuan-zeng, LIU Wen-zhong. Study on the Differentiation Regulation of miR-142 and miR-144 on Ovine Preadipocytes by Targeting FoxO1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 675-684.

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Study on the Differentiation Regulation of miR-142 and miR-144 on Ovine Preadipocytes by Targeting FoxO1 Gene

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