Wnt10b对山羊前体脂肪细胞分化相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2018.04.004

Abstract

Abstract:

The aim of this study was to investigate the effect of Wnt10b gene on the precursor adipocytes differentiation of goat by RNA interference. The goat Wnt10b siRNA sequence was designed and compounded,and the goat subcutaneous and intramuscular precursor adipocytes were obtained by collagenase digestion. The effect of Wnt10b gene expression on the cell differentiation and adipocyte differentiation marker genes expression were detected by using the effective siRNA sequence to interfere the expression of Wnt10b gene in goat subcutaneous and intramuscular precursor adipocytes.The results showed that an effective goat Wnt10b siRNA sequence was obtained,and the expression of Wnt10b was down-regulated by 63% (P<0.01) and 67% (P<0.01), respectively after transfection in goat subcutaneous and intramuscular precursor adipocytes. Results of Oil red O staining showed that the accumulation of lipid droplets were inhibited significantly by Wnt10b RNAi in goat subcutaneous and intramuscular adipocytes. The C/EBPβ, PPARγ,SREBP1 and Pref1 genes were extremely significantly down-regulated in the subcutaneous precursor adipocyte differentiation after Wnt10b RNAi (P<0.01), LPL was significantly down-regulated (P<0.05). The AP2 and LPL were extremely significantly down-regulated (P<0.01), C/EBPβ was significantly down-regulated (P<0.05), and Pref1 was extremely significantly up-regulated (P<0.01) in intramuscular precursor adipocytes differentiation. In this study, it was found that Wnt10b gene could promote the differentiation of goat subcutaneous precursor adipocytes by regulating the expression of C/EBPβ, PPARγ, SREBP1 and LPL, and promote the differentiation of goat intramuscular precursor adipocytes by regulating the expression of AP2, LPL, C/EBPβ and Pref1.

CLC Number:

S827.2

LIN Sen, LIN Ya-qiu, ZHU Jiang-jiang, XU Qing, ZHAO Yue, CHI Yong-dong, WANG Yong. Effect of Wnt10b on the Expression of Precursor Adipocytes Differentiation Related Genes in Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 685-692.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.04.004

https://www.xmsyxb.com/EN/Y2018/V49/I4/685

References

[1] NOVAKOFSKI J. Adipogenesis:usefulness of in vitro and in vivo experimental models[J]. J Anim Sci, 2004, 82(3):905-915.
[2] HARPER G S, PETHICK D W. How might marbling begin?[J]. Aust J Exp Agric, 2004, 44(7):653-662.
[3] DU M, WANG B, FU X, et al. Fetal programming in meat production[J]. Meat Sci, 2015, 109:40-47.
[4] JO J, SHREIF Z, PERIWAL V. Quantitative dynamics of adipose cells[J]. Adipocyte, 2012, 1(2):80-88.
[5] NUSSE R, CLEVERS H. Wnt/β-catenin signaling, disease, and emerging therapeutic modalities[J]. Cell, 2017, 169(6):985-999.
[6] TANAKA K, KITAGAWA Y, KADOWAKI T. Drosophila segment polarity gene product porcupine stimulates the posttranslational N-glycosylation of wingless in the endoplasmic reticulum[J]. J Biol Chem, 2012, 277(15):12816-12823.
[7] LONGO K A, WRIGHT W S, KANG S, et al. Wnt10b inhibits development of white and brown adipose tissues[J]. J Biol Chem, 2004, 279(34):35503-35509.
[8] KUEM N, SONG S J, YU R N, et al. Oleuropein attenuates visceral adiposity in high-fat diet-induced obese mice through the modulation of WNT10b-and galanin-mediated signalings[J]. Mol Nutr Food Res, 2014, 58(11):2166-2176.
[9] KARCZEWSKA-KUPCZEWSKA M, STEFANOWICZ M, Matulewicz N, et al. Wnt signaling genes in adipose tissue and skeletal muscle of humans with different degrees of insulin sensitivity[J]. J Clin Endocrinol Metab, 2016, 101(8):3079-3087.
[10] WRIGHT W S, LONGO K A, DOLINSKY V W, et al. Wnt10b inhibits obesity in ob/ob and agouti mice[J]. Diabetes, 2007, 56(2):295-303.
[11] LUO H F, WEI H K, HUANG F R, et al. The effect of linseed on intramuscular fat content and adipogenesis related genes in skeletal muscle of pigs[J]. Lipids, 2009, 44(11):999-1010.
[12] JEONG J Y, KIM J S, NGUYEN T H, et al. Wnt/β-catenin signaling and adipogenic genes are associated with intramuscular fat content in the longissimus dorsi muscle of Koreancattle[J]. Anim Genet, 2013, 44(6):627-635.
[13] WANG Y H, BYRNE K A, REVERTER A, et al. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle[J]. Mamm Genome, 2005, 16(3):201-210.
[14] 曹怡玮, 郭熙志. Wnt信号通路调控脂肪发育与代谢的研究进展[J]. 中国科学:生命科学, 2017, 47(4):355-362.
CAO Y W, GUO X Z. Wnt signaling regulates adipose tissue formation and metabolism[J]. Scientia Sinica Vitae, 2017, 47(4):355-362. (in Chinese)
[15] ROSS S E, HEMATI N, LONGO K A, et al. Inhibition of adipogenesis by Wnt signaling[J]. Science, 2000, 289(5481):950-953.
[16] CAWTHORN W P, BREE A J, YAO Y, et al. Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a β-catenin-dependent mechanism[J]. Bone, 2012, 50(2):477-489.
[17] BIRSOY K, CHEN Z, FRIEDMAN J. Transcriptional regulation of adipogenesis by KLF4[J]. Cell Metab, 2008, 7(4):339-347.
[18] PARK Y K, WANG L M, GIAMPIETRO A, et al. Distinct roles of transcription factors KLF4, Krox20 and PPARγ in adipogenesis[J]. Mol Cell Biol, 2017, 37(2):e00554-16.
[19] JIANG S Z, WEI H K, SONG T X, et al. KLF13 promotes porcine adipocyte differentiation through PPARγ activation[J]. CellBiosci, 2015, 5:28.
[20] FARMER S R. Transcriptional control of adipocyte formation[J]. Cell Metab, 2006, 4(4):263-273.
[21] TANG Q Q, ZHANG J W, LANE M D. Sequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesis[J]. Biochem Biophys Res Commun, 2004, 319(1):235-239.
[22] CHMURZYN'SKA A. The multigene family of fatty acid-binding proteins(FABPs):function, structure and polymorphism[J]. J Appl Genet, 2006, 47(1):39-48.
[23] JEON T I, OSBORNE T F. SREBPs:metabolic integrators in physiology and metabolism[J]. Trends Endocrinol Metab, 2012, 23(2):65-72.
[24] MEAD J R, IRVINE S A, RAMJI D P. Lipoprotein lipase:structure, function, regulation, and role in disease[J]. J Mol Med, 2002, 80(12):753-769.
[25] 王刚, 曾勇庆, 武英, 等. 猪肌肉组织LPL基因表达的发育性变化及其与肌内脂肪沉积关系的研究[J]. 畜牧兽医学报, 2013, 38(3):253-257.
WANG G, ZENG Y Q, WU Y, et al. The developmental changes of LPL mRNA expression in muscle and their association with intramuscular fat for pigs[J]. Acta Veterinaria et Zootechnica Sinica,2013, 38(3):253-257. (in Chinese)
[26] JEONG J, KWON E G, IM S K, et al. Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers[J]. J Anim Sci, 2012, 90(6):2044-2053.
[27] 乔永, 黄治国, 李齐发, 等. 绵羊肌肉LPL基因表达的发育性变化及其对肌内脂肪含量的影响[J]. 中国农业科学, 2007, 40(10):2323-2330.
QIAO Y, HUANG Z G, LI Q F, et al. Developmental changes of the LPL mRNA expression and the effect on IMF content in sheep muscle[J]. Scientia Agricultura Sinica, 2007, 40(10):2323-2330. (in Chinese)
[28] 王晶. 广西三黄鸡肉质性状分析及LPL、H-FABP基因表达与肌内脂肪含量的相关研究[D]. 南宁:广西大学, 2013.
WANG J. Studies on the meat quality traits and the relationship between LPL and H-FABP genes expression with intramuscular fat content in chicken[D]. Nanning:Guangxi University, 2013.(in Chinese)
[29] WANG Y H, KIM KA, KIM J H, et al. Pref-1, a preadipocyte secreted factor that inhibits adipogenesis[J]. J Nutr, 2006, 136(12):2953-2956.
[30] CHARALAMBOUS M, DA ROCHA S T, RADFORD E J, et al. DLK1/PREF1 regulates nutrient metabolism and protects from steatosis[J]. Proc Natl Acad Sci U S A, 2014, 111(45):16088-16093.
[31] TISCHENDORF F, SCHÖNE F, KIRCHHEIM U, et al. Influence of a conjugated linoleic acid mixture on growth, organ weights, carcass traits and meat quality in growing pigs[J]. J Anim Physiol Anim Nutr(Berl), 2002, 86(3-4):117-128.
[32] 张罕星, 朱晓彤, 束刚, 等. 猪肌内脂肪前体细胞与皮下脂肪前体细胞分化过程中基因差异表达分析[J]. 中国农业科学, 2008, 41(11):3760-3768.
ZHANG H X, ZHU X T, SHU G, et al. Differential mRNA expression profiles of porcine intramuscular preadipocytes compared with subcutaneous preadipocytes during differentiation[J]. Scientia Agricultura Sinica, 2008, 41(11):3760-3768. (in Chinese)
[33] SUZUKI K, IRIE M, KADOWAKI H, et al. Genetic parameter estimates of meat quality traits in Duroc pigs selected for average daily gain, longissimus muscle area, backfat thickness, and intramuscular fat content[J]. J Anim Sci, 2005, 83(9):2058-2065.
[34] KOUBA M, BONNEAU M, NOBLET J. Relative development of subcutaneous, intermuscular, and kidney fat in growing pigs with different body compositions[J]. J Anim Sci, 1999, 77(3):622-629.

Effect of Wnt10b on the Expression of Precursor Adipocytes Differentiation Related Genes in Goat

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.
[2]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[3]	MENG Qiuchi, LU Guangyu, CHEN Dingshuang, LIN Yaqiu, WANG Ruilong, ZHONG Chaosong, WANG Yong, LIU Wei, WANG Youli, LI Yanyan, LI Zhixiong. Goats GPR35 Gene Expression Characteristics Analysis and the Effect on Subcutaneous Fat Cell Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4993-5007.
[4]	HE Zhicheng, QIN Xiaochen, Lü Yongqiang, LI Xiujin, BIAN Huilong, LUO Jun, LI Cong. Multiple Stepwise Regression Analysis and Growth Curve Fitting of Body Measurement Traits in Saanen Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5301-5311.
[5]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[6]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[7]	XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.
[8]	QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.
[9]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[10]	ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.
[11]	CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.
[12]	YE Junning, DENG Ming, XUE Huiwen, LIU Guangbin, ZOU Xian, SUN Baoli, GUO Yongqing, LIU Dewu, LI Yaokun. Identification and Analysis of mRNA and lncRNA Affecting Goat Fetal Muscle Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 989-1002.
[13]	LI Xiaobo, LIU Zhanfa, LIU Yue, CHEN Qian, MA Yuehui, ZHAO Qianjun, YE Shaohui. Mining Genes Related to Wool Bending of Zhongwei Goat Based on WGCNA and GSEA [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2930-2943.
[14]	SUN Hao, ZHE Xiaoshu, ZHANG Wenqi, HAO Fei, LI Wennan, LIU Jie, LIU Dongjun. Effects of Chaetocin on Histone Methylation Modification of Cashmere Goats ADSCs [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2380-2389.
[15]	LIU Kehan, WANG Yong, LI Yanyan, WANG Chongyang, ZHU Jiangjiang, XIONG Yan, LI An, LIN Yaqiu. Effects of SRSF10 on the Differentiation of Intramuscular Preadipocytes in Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1768-1778.