硒化党参多糖和大蒜多糖协同增强鸡外周血淋巴细胞和新城疫疫苗的免疫功效

doi:10.11843/j.issn.0366-6964.2017.07.020

Abstract

Abstract:

In order to validate the synergistic immune-enhancing action of selenizing Codonopsis pilosula polysaccharide (sCPP) and garlic polysaccharide (GP) and the efficacy of their compound sCPP-GP and offer theoretical evidence for developing new-type immunopotentiators, the immune-enhancing activities of these three kinds of polysaccharide were compared. In vitro test, the effects of three polysaccharides on chicken peripheral lymphocytes proliferation and mRNA transcription of IL -2 and IFN -γ were determined respectively by MTT method and Real-time quantitative PCR technique. In vivo test, the chickens were injected with three polysaccharides when vaccination of Newcastle disease vaccine, the dynamic changes of chicken peripheral lymphocytes proliferation, serum HI antibody titer, IL-2 contents and IFN-γ contents were determined. The results of in vitro test showed that the three polysaccharides at appropriate concentrations could significantly promote lymphocytes proliferation and mRNA transcription of IL -2 and IFN -γ, and the effects of compound were significantly stronger than those of two constituents; The results of in vivo test showed that at four time points after vaccination three polysaccharides could promote lymphocytes proliferation and enhance serum HI antibody titer and contents of IL-2 and IFN-γ at different degrees, and the efficacy of compound were significantly stronger than those of constituents. These results indicate that sCPP and GP can synergistically enhance the immunological efficacy of chicken peripheral lymphocyte and Newcastle disease vaccine, sCPP-GP compound's action is stronger than its two constituents, and can be a candidate of new-type immunopotentiators.

CLC Number:

S853.74

LIU Kuan-hui, TIAN Wei-jun, GAO Zhen-zhen, HOU Ran-ran, YUE Chan-juan, WANG De-yun, LIU Jia-guo, WU Yi, HU Yuan-liang. Selenizing Codonopsis Pilosula Polysaccharide and Garlic Polysaccharide Synergistically Enhance Immunological Efficacy of Chicken Lymphocyte and Newcastle Disease Vaccine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1349-1356.

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Selenizing Codonopsis Pilosula Polysaccharide and Garlic Polysaccharide Synergistically Enhance Immunological Efficacy of Chicken Lymphocyte and Newcastle Disease Vaccine

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