纯种与杂种猪肌肉生长相关分子表达的差异分析

doi:10.11843/j.issn.0366-6964.2016.12.006

Abstract

Abstract:

To understand the molecular regulation differences of muscle growth and development in purebred and crossbred pigs, and analyze its effect on carcass and meat quality in pig. Duroc, Landrace, Large White pigs and Duroc×(Landrace×Large White) crossbred pigs with the average body weight of 50 kg were used. Each group was repeated by 6 individuals, and longissimus dorsi muscle was collected and analyzed at both phenotypic and molecular levels. The results showed that, compared with the purebred pigs, crossbred pigs had significant higher myofibre cross-sectional area, white myofibre ratio, lactate dehydrogenase A gene expression level and mtDNA copy number (P<0.01); On the whole, the expression level of IGF1, PDK1 and GLUT4 genes promoting myofibre growth and development in crossbred pigs were significantly increased (P<0.05), the expression level of MSTN and FOXO1 genes inhibiting myofibre growth and development in crossbred pigs were significantly decreased (P<0.05); the expression level of PGC1A gene promoting white myofibre conversion in crossbred pigs was significantly increased (P<0.05), the expression level of MEF2A, MYOZ1, MRF4 and NFATC1 genes inhibiting white myofibre conversion in crossbred pigs were significantly decreased (P<0.05); the expression level of miR-1 and miR-133 related to myofibre proliferation in crossbred pigs were significantly increased (P<0.01), while the expression level of miR-499 inhibiting white myofibre growth in crossbred pigs was significantly decreased (P<0.01). These results suggest that improving carcass traits in pigs through corssbreeding is achieved by changing its molecular regulation level, which had adverse effects on meat quality.

CLC Number:

S828
S813.2

ZHANG Jie, LUO Zong-gang. The Molecular Expression Differences of Muscle Growth in Purebred and Crossbred Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2370-2378.

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The Molecular Expression Differences of Muscle Growth in Purebred and Crossbred Pigs

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