FSH及Wnt信号通路对绵羊卵泡颗粒细胞的增殖及雌激素分泌的影响

doi:10.11843/j.issn.0366-6964.2016.01.009

Abstract

Abstract:

This study aims to investigate the effect of Wnt signaling pathway on proliferation and estrogen secretion of sheep granulosa cells and to explore whether the effect was related to FSH(Follicular stimulating hormone，FSH).With or without FSH，sheep granulosa cells were cultured in medium with different concentrations of Wnt signaling pathway inhibitor IWR-1 for 168 h.Then，Guava ViaCount^®Reagent was used to detect the changes of cell number and sheep ELISA kit was used to detect the changes of estrogen concentrations.The relative expression of CYP19 and CCND2，which are the target genes of FSH，was analyzed by qRT-PCR.The results showed that IWR-1 could cause a significant(P<0.05) reduction in the number of granule cells whether FSH existed or not.With the presence of FSH，the addition of IWR-1 could significantly(P<0.05) decrease estrogen concentration.When the concentration of IWR-1 was 1.0 μmol•L^-1，its inhibitory effect on granulosa cells was of most obvious.Besides，IWR-1 decreased the abundance of CYP19 mRNA and CCND2 mRNA.In conclusion，Wnt signaling pathway can improve the biological function of sheep granulosa cells，and this function may be related to FSH.

CLC Number:

S826.2

ZHAO Miao-miao，JIANG Xiao-long，CHEN Jian-wei，HUANG Yang，YAO Xiao-lei，CAO Xia，LI Peng-fei，Lü Li-hua. Effects of FSH and Wnt Signaling Pathway on Proliferation and Estrogen Secretion of Sheep Granulosa Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 64-70.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.01.009

https://www.xmsyxb.com/EN/Y2016/V47/I1/64

References

［1］NUSSE R，VARMUS H E.Many tumors induced by the mouse mammary tumor virus contain a provirns integrated in the same region of the host genome［J］.Cell，1982，31(1)：99-109.
［2］SEGDITSAS S，TOMLINSON I.Colorectal cancer and genetic alterations in the Wnt pathway［J］.Oncogene，2006，25(57)：7531-7537.
［3］WODARZ A，NUSSE R.Mechanisms of Wnt signaling in development［J］.Annu Rev Cell Dev Biol，1998,14：59-88.
［4］KIKUCHI A，YAMAMOTO H，KISHIDA S.Multiplicity of the interactions of Wnt proteins and their receptors［J］. Cell Signal，2007,19(4)：659-671.
［5］尹定子，宋海云.Wnt信号通路:调控机理和生物学意义［J］.中国细胞生物学学报，2011，33(2)：103-111.
YIN D Z，SONG H Y.Regulation of Wnt signaling:Mechanism and Biological significance［J］. Chinese Journal of Cell Biology，2011，33(2)：103-111.(in Chinese)
［6］刘烜，王海生，刘淑萍.Wnt信号转导通路与细胞凋亡［J］.内蒙古医学杂志，2011，43(1)：50-53.
LIU X，WANG H S，LIU S P.Wnt signal transduction pathway and apoptosis［J］.Inner Mongolia Medical Journal，2011，43(1)：50-53.(in Chinese)
［7］WILLERT K，BROWN J D，DANENBERG E，et al.Wnt proteins are lipid-modified and can act as stem cell growth factors［J］.Nature，2003，423(6938)：448-452.
［8］BOYER A，GOFF A K，BOERBOOM D.WNT signaling in ovarian follicle biology and tumorigenesis［J］. Trends Endocrinol Metab，2010，21(1)：25-32.
［9］CHEN B，DODGE M E，TANG W，et al.Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer［J］.Nat Chem Biol， 2009，5(2)：100-107.
［10］LU J，MA Z，HSIEH J C，et al.Structure-activity relationship studies of small-molecule inhibitors of Wnt response［J］.Bioorg Med Chem Lett，2009，19(14)：3825-3827.
［11］GUPTA P S，FOLGER J K,RAJPUT S K，et al.Regulation and regulatory role of WNT signaling in potentiating FSH action during bovine dominant follicle selection［J］.PLoS One，9(6)：e100201.
［12］VAINIO S，HEIKKILÄ M，KISPERT A，et al.Female development in mammals is regulated by Wnt-4 signalling［J］.Nature，1999，397（6718）：405-409.
［13］BOERBOOM D，PAQUET M，HSIEH M，et al.Misregulated Wnt/beta-catenin signaling leads to ovarian granulosa cell tumor development［J］.Cancer Res，2005，65（20）：9206-9215.
［14］高庆华，周虚，王春清，等.促卵泡激素和胰岛素对牛卵泡颗粒细胞长期培养的影响［J］.中国兽医学报，2006，26(4)：442-443，447.
GAO Q H，ZHOU X，WANG C Q，et al.Effect of FSH and insulin on bovine granulosa cells in long term serum-cultrue［J］. Chinese Journal of Veterinary Science，2006，26(4)：442-443，447.(in Chinese)
［15］李鹏飞，岳文斌，李富禄，等.可卡因-苯丙胺调控转录肽(CART)对猪卵巢卵泡颗粒细胞雌激素产生的影响［J］.畜牧兽医学报，2012，43(12)：1879-1886.
LI P F，YUE W B，LI F L，et al.Effects of CART on estradiol production of pig ovarian follicular granulosa cells in vitro culture［J］.Acta Veterinaria et Zootechnica Sinica，2012，43(12)：1879-1886.(in Chinese)
［16］姜晓龙.DAPT 阻断Notch信号通路对绵羊卵泡颗粒细胞功能的影响［D］.太谷：山西农业大学,2013.
JIANG X L.Effect of Notch signaling pathway blocked with DAPT on function of sheep granulosa cells in vitro［D］.Taigu：Shanxi Agricultural University,2013.(in Chinese)
［17］王锋.卵泡生长发育成熟及其内分泌调控［J］.动物医学进展，1994，19(1)：1-8.
WANG F.Mature follicle growth and endocrine regulation［J］.Progress in Veterinary Medicine，1994，19(1)：1-8.(in Chinese)
［18］SEN A，LÜ L，BELLO N，et al.Cocaine- and amphetamine-regulated transcript accelerates termination of follicle-stimulating hormone-induced extracellulary regulated kinase 1/2 and Akt activation by regulating the expression and degradation of specific mitogen-activated protein kinase phosphatases in bovine granulosa cells［J］.Mol Endocrinol，2008，22(12)：2655-2676.
［19］LÜ L H，JIMENEZ-KRASSEL F，SEN A，et al.Evidence supporting a role for cocaine- and amphetamineregulated transcript(CARTPT) in control of granulosa cell estradiol production associated with dominant follicle selection in cattle［J］.Biol Reprod，2009，81(3)：580-586.
［20］HUGHES F M J R，GOROSPE W C.Biochemical identification of apoptosis(programmed cell death) in granulosa cells：evidence for a potential mechanism underlying follicular atresia［J］.Endocrinology，1991，129(5)：2415-2422.
［21］LÜ L H，JIMENEZ-KRASSEL F，SEN A，et al.Evidence for a local inhibitory effect of the CART peptide on LH-induced thecal and rostenedione production［J］.Biol Reprod，2007，77(Suppl 1)：179.
［22］HUNZICKER-DUNN M，MAIZELS E T.FSH signaling pathways in immature granulosa cells that regulate target gene expression：branching out from protein kinase A［J］.Cell Signal，2006，18(9)：1351-1359.
［23］CAMPBELL B K,SCARAMUZZI R J,WEBB R.Induction and maintenance of oestradiol and immunoreactive inhibin production with FSH by ovine granulosa cells cultured in serum-free media［J］.J Reprod Fertil，1996，106(1)：7-16.
［24］李鹏飞，岳文斌，庞钰莹，等.FSH和胰岛素对绵羊卵巢卵泡颗粒细胞体外培养的影响［J］.畜牧兽医学报，2013，44(9)：1386-1391.
LI P F，YUE W B，PANG Y Y，et al.Effects of FSH and insulin on sheep ovarian follicular granulosa cells in vitro culture［J］.Acta Veterinaria et Zootechnica Sinica，2013，44(9)：1386-1391.(in Chinese)
［25］LAPOINTE E，BOERBOOM D.WNT signaling and the regulation of ovarian steroidogenesis［J］.Front Biosci （Schol Ed），2011，3：276-285.
［26］ZHAO Y，MENDELSON C R，SIMPSON E R.Characterization of the sequences of the human CYP19(aromatase) gene that mediate regulation by glucocorticoids in adipose stromal cells and fetal hepatocytes［J］.Mol Endocrinol，1995，9(3)：340-349.
［27］LUO W，WILTBANK M C.Distinct regulation by steroids of messenger RNAs for FSHR and CYP19A1 in bovine granulosa cells［J］.Biol Reprod，2006，75(2)：217-225.
［28］HAN Y，XIA G，TSANG B K.Regulation of cyclin D2 expression and degradation by follicle-stimulating hormone during rat granulosa cell proliferation in vitro［J］.Biol Reprod，2013，88(3)：57.
［29］SEN A，BETTEGOWDA A，JIMENEZ-KRASSEL F，et al.Cocaine-and amphetamine regulated transcript regulation of follicle-stimulating hormone signal transduction in bovine granulosa cells［J］. Endocrinology，2007，148(9)：4400-4410.

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[3]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[4]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[5]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[6]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[7]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[8]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[9]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[10]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.
[11]	LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.
[12]	ZHOU Shiwei, WU Tingjie, WANG Qian, CAI Bei, HAN Saizheng, WU Yanhu, WANG Xiaolong, CHEN Yulin. Establishment of the Efficient Method to Detect the FecB Mutation and the Construction of the Prolific Tan Sheep Population [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2920-2929.
[13]	DING Ziqiang, GE Wenbo, DUAN Hongwei, LÜ Jianshu, ZENG Jianlin, WANG Wenjuan, NIU Tian, ZHANG Yong, ZHAO Xingxu, HU Junjie. Dihydrotestosterone Affects Sheep Uterine Function through Regulating Progesterone, Oestrogen and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2993-3005.
[14]	LI Tingting, LIU Qiuyue, LI Xiangchen, WANG Haitao. Research Progress and Applications of Genes Associated with Economic Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2417-2434.
[15]	XU Songwei, XIANG Hua, ZHANG Huanrong, YANG Lu, WANG Xianjun, SALANG Wenzhu, ZHU Jiangjiang. Effects of Interfering SERBP1 on the Cell Proliferation, Cell Cycle and Apoptosis in Goat Turbinate Bone Primary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2708-2720.

Effects of FSH and Wnt Signaling Pathway on Proliferation and Estrogen Secretion of Sheep Granulosa Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments