不同月龄高原型藏绵羊睾丸组织中蛋白基因产物9.5和神经肽Y的分布比较

doi:10.11843/j.issn.0366-6964.2016.01.010

Abstract

Abstract:

This experiment was conducted to investigate the distribution of gene product 9.5 (PGP 9.5) and neuropeptide Y (NPY) in Tibetan Plateau sheep testis and in an attempt to explore their relationships with testicular spermatogenesis at different ages.Testis from 0.5-，2-，5-，8-，12-and 18-month-old Tibetan Plateau sheep were sectioned and immunostained with antisera to the NPY and PGP 9.5.Furthermore，IPP (Image-Pro Plus) statistics methods were used to compare the average absorbance of PGP9.5 and NPY expression in all of these testis.The testicular parenchyma did not show any NPY- and PGP9.5-containing immunoreactivity in 0.5-month-old sheep，and very scattered immunoreactivities were present within the gonocytes，but the expression of PGP9.5 in testis of 2-month-old sheep was significantly stronger than NPY at the same age (P<0.01)，both of the PGP9.5 and NPY were strongly present in 18-month-old sheep(P<0.01).By contrast，no expression of NPY and PGP9.5 immunoreactivities were observed within the primary spermatocytes in 18-month-old sheep.The expression of PGP9.5 in Sertoli cells underlie age-related declines，which was poorly investigated at 12-month-old and no expression at 18-month-old，but they were obviously detected in Leydig cells and vscellum at different ages.The NPY expression in Leydig cells were also poorly investigated，and were mainly expressed in Sertoli cells in 2-month-old sheep testis，and after that，the expression of NPY in vscellum with a manner of age-related enhances.The results suggest that the PGP9.5 and NPY expression was significantly difference in Tibetan Plateau sheep testis at different ages might serve as regulators for the testicular functions，also the expression of PGP9.5 in Leydig cells in the testis reflect the functional regulates of testosterone.Namely，the distribution of NPY in the wall of testis vascular significantly stronger in a manner of age-related increase may provide new indexes for the research of plateau adaptability mechanism in testicular blood regulation，and play a role in the local microcirculation and the interactions with the Sertoli cells.

CLC Number:

S826.2

YUAN Li-gang，QU Ya-ling，LU Yu-rong，LI Cong. Distribution of Protein Gene Product 9.5 and Neuropeptide Y in Tibetan Plateau Sheep Testis at Different Ages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 71-78.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.01.010

https://www.xmsyxb.com/EN/Y2016/V47/I1/71

References

［1］TAINIO H.Peptidergic innervation of the human testis and epididymis［J］Acta Histochem，1994，96(4)：415-420.
［2］GONG Y G，FENG M M，HU X N，et al.Peptidergic not monoaminergic fibers profusely innervate the young adult human testis［J］.J Anat，2009，214(3)：330-338.
［3］WROBEL K H，ABU-GHALI N.Autonomic innervation of the bovine testis［J］. Acta Anat(Basel)，1997，160(1)：1-14.
［4］WROBEL K H，BRANDL B.The autonomous innervation of the porcine testis in the period from birth to adulthood［J］.Ann Anat，1998，180(2)：145-156.
［5］袁莉刚，曲亚玲，谷来凤，等.不同年龄牦牛睾丸蛋白基因产物9.5和神经肽Y的分布比较［J］.解剖学报，2015，46(2)：232-237.
YUAN L G，QU Y L，GU L F，et al.Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages［J］.Acta Anatomica Sinica， 2015，46(2)：232-237.(in Chinese)
［6］袁莉刚，曲亚玲，谷来凤，等.蛋白基因产物9.5在高原黄牛和平原黄牛睾丸的分布情况的比较［J］.中国兽医科学，2014，44(9)：981-986.
YUAN L G，QU Y L，GU L F，et al.Comparison of distribution of protein gene product 9.5 in bovine testis from plateau with that from plain［J］.Chinese Veterinary Science，2014，44(9)：981-986.(in Chinese)
［7］谷来凤，袁莉刚，朱俊峰，等.平原黄牛和高原黄牛睾丸神经肽Y表达的比较观察［J］.甘肃农业大学学报，2014，49(3)：23-27.
GU L F，YUAN L G，ZHU J F，et al Comparative observation of neuropeptide Y in testis between plain and plateau cattle (Bos taurus)［J］.Journal of Gansu Agricultural University，2014，49(3)：23-27.(in Chinese)
［8］袁莉刚，孙英，黄布敏.成年高原藏羊精索血管分布的形态学研究［J］.畜牧兽医学报，2011，42(10)：1450-1456.
YUAN L G，SUN Y，HUANG B M.Micromorphological characteristics on the adult Tibetan plateau sheep spermatic cord［J］.Acta Veterinaria et Zootechnica Sinica，2011，42(10)：1450-1456.(in Chinese)
［9］孙英，袁莉刚，赵海涛.成年高原藏羊睾丸小叶内微血管分布和形态特征［J］.解剖学报，2011，42(1)：114-118.
SUN Y，YUAN L G，ZHAO H T.Distribution and morphological characteristics of testis lobule micrangium in adult Tibetan plateau sheep［J］.Acta Anatomica Sinica，2011，42(1)：114-118.(in Chinese)
［10］GRUENEWALD D A，NAAI M A，MARCK B T，et al.Age-related decrease in neuropeptide-Y gene expression in the arcuate nucleus of the male rat brain is independent of testicular feedback［J］.Endocrinology，1994，134(6)：2383-2389.
［11］POZUELO J M，RODRÍGUEZ R，ARRIAZU R，et al.Changes in the number and volume of NPY and VIP neurons from periprostatic accessory vegetative ganglia in pre-and peripubertal rats.A stereological study［J］.Tissue Cell， 2010，42(1)：1-8.
［12］DI YORIO M P，DELGADIN T H，PÉREZ SIRKIN D I，et al.Growth hormone，luteinizing hormone，and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish，Cichlasoma dimerus［J］.Fish Physiol Biochem，2015，14(4)：843-852.
［13］HEIDARI B，RAHMATI-AHMADABADI M，AKHONDI M M，et al.Isolation，identification，and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers［J］.J Assist Reprod Genet，2012，29(10)：1029-1038.
［14］HEIDARI B，GIFANI M，SHIRAZI A，et al.Enrichment of undifferentiated type a spermatogonia from goat testis using discontinuous percoll density gradient and differential plating［J］.Avicenna J Med Biotechnol，2014，6(2)：94-103.
［15］LEE K H，LEE R，LEE W Y，et al.Identification and in vitro derivation of spermatogonia in beagle testis［J］.PLoS One，2014，9(10)：e109963.
［16］LEE W Y，LEE K H，HEO Y T，et al.Transcriptional coactivator undifferentiated embryonic cell transcription factor 1 expressed in spermatogonial stem cells：a putative marker of boar spermatogonia［J］.Anim Reprod Sci，2014，150(3-4)：115-124.
［17］GARCIA T X，FARMAHA J K，KOW S，et al.RBPJ in mouse Sertoli cells is required for proper regulation of the testis stem cell niche［J］.Development，2014，141(23)：4468-4478.
［18］KON Y，ENDOH D，IWANAGA T.Expression of protein gene product 9.5，a neuronal ubiquitin C-terminal hydrolase，and its developing change in sertoli cells of mouse testis［J］.Mol Reprod Dev，1999，54(4)：333-341.
［19］TENGOWSKI M W，FENG D，SUTOVSKY M，et al.Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline-or 1，3-dinitrobenzene-exposed rats［J］.Biol Reprod，2007，76(1)：149-163.

［20］袁莉刚，闫振龙，陶金忠，等.PGP9.5和神经肽Y在双峰驼正常睾丸和隐睾的分布比较［J］.畜牧兽医学报，2015，46(2)：303-308.
YUAN L G，YAN Z L，TAO J Z，et al.The distribution of PGP9.5 and NPY in the normal testis and cryptorchidism of Bactrian camel［J］.Acta Veterinaria et Zootechnica Sinica，2015，46(2)：303-308.(in Chinese)
［21］KANZAKI M，FUJISAWA M，OKUDA Y，et al.Expression and regulation of neuropeptide Y messenger ribonucleic acid in cultured immature rat Leydig and Sertoli cells［J］.Endocrinology，1996，137(4)：1249-1257.
［22］LIAO W，CAI M，CHEN J，et al.Hypobaric hypoxia causes deleterious effects on spermatogenesis in rats［J］.Reproduction，2010，139(6)：1031-1038.
［23］TEERDS K J，HUHTANIEMI I T.Morphological and functional maturation of Leydig cells：from rodent models to primates［J］.Hum Reprod Update，2015，21(3)：310-328.
［24］ALLEN C D，WASER B，KRNER M，et al.Neuropeptide Y acts within the rat testis to inhibit testosterone secretion［J］.Neuropeptides，2011，45(1)：55-61.
［25］KRNER M，WASER B，THALMANN G N，et al.High expression of NPY receptors in the human testis［J］.Mol Cell Endocrinol，2011，337(1-2)：62-70.
［26］SALEH A M，ALAMELDIN M A，ABDELMONIEM M E，et al.Immunohistochemical investigations of the autonomous nerve distribution in the testis of the camel(Camelus dromedarius)［J］.Ann Anat，2002，184(3)：209-220.
［27］陈国娟.牦牛睾丸局部调节因子增龄性与季节性变化的表达研究［D］.兰州：甘肃农业大学，2015.
CHEN G J.The expression study of the local regulation factor in yak testis associated with age and season［D］.Lanzhou：Gansu Agricultural University，2015.(in Chinese)

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[3]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[4]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[5]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[6]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[7]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[8]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[9]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[10]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.
[11]	LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.
[12]	ZHOU Shiwei, WU Tingjie, WANG Qian, CAI Bei, HAN Saizheng, WU Yanhu, WANG Xiaolong, CHEN Yulin. Establishment of the Efficient Method to Detect the FecB Mutation and the Construction of the Prolific Tan Sheep Population [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2920-2929.
[13]	DING Ziqiang, GE Wenbo, DUAN Hongwei, LÜ Jianshu, ZENG Jianlin, WANG Wenjuan, NIU Tian, ZHANG Yong, ZHAO Xingxu, HU Junjie. Dihydrotestosterone Affects Sheep Uterine Function through Regulating Progesterone, Oestrogen and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2993-3005.
[14]	LI Tingting, LIU Qiuyue, LI Xiangchen, WANG Haitao. Research Progress and Applications of Genes Associated with Economic Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2417-2434.
[15]	XU Songwei, XIANG Hua, ZHANG Huanrong, YANG Lu, WANG Xianjun, SALANG Wenzhu, ZHU Jiangjiang. Effects of Interfering SERBP1 on the Cell Proliferation, Cell Cycle and Apoptosis in Goat Turbinate Bone Primary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2708-2720.

Distribution of Protein Gene Product 9.5 and Neuropeptide Y in Tibetan Plateau Sheep Testis at Different Ages

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments