供体牛外周血AMH浓度对荷斯坦青年奶牛体内胚胎生产效率的影响

doi:10.11843/j.issn.0366-6964.2021.06.014

摘要/Abstract

摘要： 旨在研究供体牛超数排卵（简称超排）过程中不同时间外周血AMH浓度与荷斯坦青年奶牛体内胚胎生产效率的相关性，优化供体牛筛选标准，提高荷斯坦青年奶牛体内胚胎生产效率。本试验共选用96头荷斯坦青年奶牛进行超排处理，分别在孕酮阴道栓（CIDR）埋置、人工授精和胚胎回收当天通过尾根采集外周血检测抗缪勒管激素（anti-Müllerian hormone，AMH）浓度，将不同时期的外周血AMH浓度按照高（浓度分布最高的25%）、中等（浓度分布中间的25%~75%）、低（浓度分布最低的25%）分为3组，并检验了这3组之间头均回收胚胎数、头均可用胚胎数、头均退化胚胎数和头均未受精数等指标间的差异。结果表明：1）外周血AMH浓度检测结果显示，从CIDR埋置至胚胎回收的全过程AMH浓度整体呈下降趋势；2）CIDR埋置当天AMH高浓度组头均回收胚胎数（9.38±1.24）显著高于中等浓度组（6.47±0.56）（P<0.05），说明CIDR埋置当天AMH浓度高的供体牛超排效果更好，回收胚胎数更多；但是，结果也表明，高浓度组头均未受精卵母细胞数显著高于其他两组（P<0.05），说明部分卵母细胞未能成功受精，这可能是由于高浓度组牛只更多的卵泡优势化，排卵周期延长导致部分卵母细胞未能完成正常受精；3）人工授精和胚胎回收当天的AMH数据分析结果均表明，3组间各项胚胎生产指标均无显著性差异（P>0.05）；4）通过对供体牛体重记录数据分析发现，不同体重牛只间的体内胚胎生产效率差异也不显著（P>0.05），但是体况适中组牛只的可用胚胎数、回收胚胎数优于其他两组，尤其是优于体重偏胖组。因此，CIDR埋置时，外周血AMH浓度可以作为青年荷斯坦供体牛筛选标准之一，且供体牛选择时宜选用体况适中的牛只。

关键词: 抗缪勒管激素, 超数排卵, 胚胎生产, 供体牛筛选

Abstract: In order to improve in vivo embryo production efficiency and optimize the screening criteria for donor Holstein cows, this study analyzed the correlation between the peripheral blood AMH concentration with embryo production efficiency in Holstein young cows during the process of superovulation of donor cattle. In this experiment, 96 Holstein young cows were selected, after superovulation, peripheral blood was collected from caudal roots on the day of CIDR implantation, artificial insemination and embryo recovery to detect AMH concentration, and divided into 3 groups according to high (25% of the highest concentration distribution), medium (25%-75% of the middle concentration distribution) and low (25% of the lowest concentration distribution) AMH concentration. The difference in the average embryos number per donor, average usable embryos number per donor, average degenerated embryos number per donor and average unfertilized oocytes number per donor among 3 groups were compared and analyzed. The results showed that:1) The detection results of AMH concentration in peripheral blood showed that the concentration of AMH decreased from CIDR implantation to embryo recovery. 2) On the day of CIDR implantation, the average embryos number per donor in AMH high-concentration group (9.38±1.24) was significantly higher than that in AMH medium-concentration group(6.47±0.56, P<0.05), indicating that donor cattle with high AMH concentration on the day of CIDR implantation had better superovulation effect and more embryos recovered; However, the average unfertilized oocytes number per donor in AMH high-concentration group was significantly higher than that in other two groups (P<0.05); It means that some oocytes failed to be fertilized, which might be due to the fact that more follicles of cows in the high-concentration group dominated, and the prolonged ovulation cycle caused some oocytes to fail to complete normal fertilization. 3) The results of AMH data analysis on the day of artificial insemination and embryo recovery showed that there were no significant differences in various embryo production indicators among the 3 groups (P>0.05). 4) The effect of body weight of donor cattle on the in vivo embryo production efficiency was investigated, the results showed that the difference in embryo production efficiency among individuals with different body weight was not significant (P>0.05), the number of available embryos and the number of retrieved embryos of cows in moderate body condition group were higher than that in other two groups, especially higher than that in the over-weight group. Therefore, the concentration of AMH in peripheral blood on the day of CIDR implantation can be used as one of the screening criteria for donor cattle, and the donor heifer should own appropriate body condition score.

Key words: AMH, superovulation, embryo production, donor cattle selection

中图分类号:

S823.2

李树静, 冯春涛, 胡智辉, 朱化彬, 余文莉, 赵善江. 供体牛外周血AMH浓度对荷斯坦青年奶牛体内胚胎生产效率的影响[J]. 畜牧兽医学报, 2021, 52(6): 1594-1602.

LI Shujing, FENG Chuntao, HU Zhihui, ZHU Huabin, YU Wenli, ZHAO Shanjiang. The Effect of Peripheral Blood AMH Concentration on Superovulation and Embryo Production Performance of Holstein Young Dairy Cattle[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1594-1602.

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