猪源携带tet(X4)基因大肠杆菌的消毒剂抗性分析

doi:10.11843/j.issn.0366-6964.2024.12.049

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (12): 5880-5885.doi: 10.11843/j.issn.0366-6964.2024.12.049

• 研究简报 • 上一篇

猪源携带tet(X4)基因大肠杆菌的消毒剂抗性分析

潘柄霖¹(), 王思楠¹, 姚国忠², 翟瑞东¹, 宋厚辉¹, 程昌勇¹^,*(), 雷蕾¹^,*()

1. 浙江农林大学动物科技学院·动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室动物健康互联网检测技术浙江省工程实验室动物医学与健康管理浙江省国际科技合作基地中澳动物健康大数据分析联合实验室, 杭州 311300
2. 杭州市余杭区农业农村局, 杭州 310023

收稿日期:2023-10-24 出版日期:2024-12-23 发布日期:2024-12-27
通讯作者: 程昌勇,雷蕾 E-mail:xianyuren@stu.zafu.edu.cn;Lamge@zafu.edu.cn;leilei910@zafu.edu.cn
作者简介:潘柄霖(1998-), 男, 四川达州人, 硕士生, 主要从事细菌耐药性研究, E-mail: xianyuren@stu.zafu.edu.cn
基金资助:
浙江省重点研发计划(2021C02058);宁波市公益类科技项目(2022S006)

Disinfectant Susceptibility Analysis of tet(X4)-Containing Porcine Escherichia coli

PAN Binglin¹(), WANG Sinan¹, YAO Guozhong², ZHAI Ruidong¹, SONG Houhui¹, CHENG Changyong¹^,*(), LEI Lei¹^,*()

1. China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China
2. Yuhang Agricultural Bureau, Hangzhou 310023, China

Received:2023-10-24 Online:2024-12-23 Published:2024-12-27
Contact: CHENG Changyong, LEI Lei E-mail:xianyuren@stu.zafu.edu.cn;Lamge@zafu.edu.cn;leilei910@zafu.edu.cn

摘要/Abstract

摘要：

旨在分析猪源粪便样本中携带tet(X4)基因大肠杆菌的分布及其耐药性、消毒剂抗性和消毒剂抗性基因携带情况。采集浙江省杭州、诸暨、舟山生猪养殖场猪粪便样本共计440份，通过PCR检测tet(X4)基因、消毒剂基因和Enterobacterial repetitive intergenic consensus(ERIC)分型；通过16S rRNA序列鉴定细菌种属，采用微量肉汤稀释法测试菌株的耐药表型和消毒剂抗性表型。结果显示，3个地区共分离到携带tet(X4)基因的大肠杆菌117株，不同地区的tet(X4)大肠杆菌分离率为24.0%~29.5%。所有菌株对氨苄西林、阿莫西林/克拉维酸、氟苯尼考、替加环素耐药率均为100%，但均对黏菌素和美罗培南敏感。菌株对次氯酸钠、过硫酸氢钾的最小抑菌浓度(minimum inhibitory concentration, MIC)值较高，均为消毒剂工作浓度的1/2。与ATCC 25922相比，仅少量菌株对过氧化氢等5种消毒剂的MIC值高于标准菌株，但92.3%的菌株对苯扎溴铵的MIC值高于标准菌株。菌株所携带的消毒剂抗性基因以sugE(c)、qacF和qacEΔ1最常见，携带率分别为100%、25.6%和11.1%。ERIC分型显示117株菌株主要分为9个类群，呈现多样性。综上，携带tet(X4)基因的大肠杆菌主要优势类群在浙江省杭州、诸暨、舟山地区均有分布且均呈现多重耐药性，菌株有消毒剂抗性基因检出但对所试消毒剂的工作浓度均不耐受，养殖场可根据消毒效果选用消毒剂阻断场内多重耐药菌的传播。

关键词: tet(X4), 大肠杆菌, 耐药性, 消毒剂抗性

Abstract:

This study aimed to asseess the prevalence of tet(X4)-containing E. coli, along with their antimicrobial resistance and disinfectant susceptibility profiles, as well as the presence of disinfectant resistance genes, in E. coli strains isolated from pig farms. A total of 440 pig fecal samples were collected and analyzed from pig farms located in Hangzhou, Zhuji, Zhoushan, within Zhejiang province. The presence of tet(X4) and disinfectant resistance genes was determined by PCR. Strain clustering was performed through Enterobacterial repetitive intergenic consensus (ERIC)-PCR, and species identification was confirmed using Sanger sequencing of the 16S rRNA gene. The phenotype of antimicrobial susceptibility and disinfectant resistance were exanimated using microdilution broth method. The results revealed that 117 tet(X4)-positive E. coli strains were isolated from samples collected across the three cities in Zhejiang province, with proportions ranging from 24.0% to 29.5%. These strains exhibited 100% resistance to ampicillin, amoxicillin/clavulanate, florfenicol, tigecycline, but remained sensitive to colistin and meropenem. Notably, the minimum inhibitory concentration (MIC) of sodium hypochlorite and potassium hydrogen peroxymonosulfate were higher than other disinfectant, reacing half of their working concentrations. Compared to ATCC 25922, only a few strains displayed higher MICs for hydrogen peroxide, peracetic acid, chlorhexidine acetate, chlorhexidine, benzalkonium bromide solution than the standard strains. Of note, 92.3% of isolates showed higher MICs for benzalkonium bromide than ATCC 25922. The predominant disinfectant resistance genes in tet(X4)-carrying E. coli were sugE(c), qacF and qacEΔ1, with detection rates of 100%, 25.6%, 11.1%, respectively. ERIC clustering showed that the 117 strains were mainly divided in to 9 groups. The study underscore the multidrug resistance observed in tet(X4)-containing E. coli strains across the three cities. While many of these strains harbored disinfectant resistance genes, they were sensitive to working concentration of tested disinfectant. Consequently, pig farms can make informed decisions regarding disinfectant selection to mitigate the spread of multi-drug resistance bacteria.

Key words: tet(X4), E. coli, antimicrobial resistance, disinfectant resistance

中图分类号:

S852.612

潘柄霖, 王思楠, 姚国忠, 翟瑞东, 宋厚辉, 程昌勇, 雷蕾. 猪源携带tet(X4)基因大肠杆菌的消毒剂抗性分析[J]. 畜牧兽医学报, 2024, 55(12): 5880-5885.

PAN Binglin, WANG Sinan, YAO Guozhong, ZHAI Ruidong, SONG Houhui, CHENG Changyong, LEI Lei. Disinfectant Susceptibility Analysis of tet(X4)-Containing Porcine Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5880-5885.

图/表 3

参考文献 17

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猪源携带tet(X4)基因大肠杆菌的消毒剂抗性分析

Disinfectant Susceptibility Analysis of tet(X4)-Containing Porcine Escherichia coli

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