一株羊源尸毒梭菌的鉴定及生物学特性研究

doi:10.11843/j.issn.0366-6964.2020.02.022

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (2): 399-403.doi: 10.11843/j.issn.0366-6964.2020.02.022

一株羊源尸毒梭菌的鉴定及生物学特性研究

豆朋朋^1,3,4, 魏勇², 王利^1,3,4*

1. 西南民族大学生命科学与技术学院, 成都 610041;
2. 四川省畜牧科学研究院, 成都 610041;
3. 西南民族大学青藏高原动物遗传资源保护与利用教育部重点实验室, 成都 610041;
4. 西南民族大学青藏高原动物遗传资源保护与利用四川省重点实验室, 成都 610041

收稿日期:2019-08-26 出版日期:2020-02-23 发布日期:2020-02-22
通讯作者: 王利,主要从事微生物遗传学研究,E-mail:qinxin916@aliyun.com
作者简介:豆朋朋(1994-),男,河北沧州人,硕士生,主要从事动物疫病防控研究,E-mail:2862390172@qq.com
基金资助:
四川省肉羊创新团队防疫岗位；农业农村部农业重大技术协同推广计划试点四川省肉羊高效生产配套技术推广应用

Identification and Biological Characteristics of Clostridium cadaveris Isolated from Goat

DOU Pengpeng^1,3,4, WEI Yong², WANG Li^1,3,4*

1. College of Life Sciences and Technology, Southwest Minzu University, Chengdu 610041, China;
2. Sichuan Institute of Animal Husbandry Science, Chengdu 610041, China;
3. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Southwest Minzu University, Chengdu 610041, China;
4. Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization Key Laboratory of Sichuan Province, Southwest Minzu University, Chengdu 610041, China

Received:2019-08-26 Online:2020-02-23 Published:2020-02-22

摘要/Abstract

摘要： 为探究羊源尸毒梭菌的生物学基本特性，进行生理生化分析、16S rDNA基因扩增、动物回感试验、耐药表型和耐药基因检测等试验。结果显示，菌株LJH-1为革兰阳性菌。PCR扩增获得长度为1 023 bp的片段。LJH-1与尸毒梭菌C4菌株聚类，从而判定LJH-1为尸毒梭菌。试验组小鼠全部死亡。小鼠肝中央静脉充盈大量红细胞，炎性细胞浸润，肾间质血管充血，肠黏膜上皮细胞脱落。LJH-1对头孢噻肟、氨苄西林等敏感，对头孢拉定、阿莫西林等耐药，并检出ant（3″）-Ia、aac（3）-IIa、aph（3'）-IIa、Sul2、Sul3和TEM耐药基因。这为该细菌性疾病的检测和防治提供了科学资料。

关键词: 尸毒梭菌, 鉴定, 致病性, 耐药性

Abstract: To explore the biological characteristics of Clostridium cadaveris isolated from goat, physiological and biochemical analysis, 16S rDNA gene amplification, animal infection test, drug-resistant phenotype and genetic testing for drug resistance of LJH-1 strain were carried out. The results showed that strain LJH-1 was Gram-positive bacteria. A fragment of 1 023 bp was obtained by PCR amplification. LJH-1 and Clostridium cadaveris were clustered, so it was identified as Clostridium cadaveris. All mice in the experimental group died. There were many red blood cells and inflammatory cells in the central hepatic lobular vein of the mice. Blood vessels in the renal stroma were congested. Intestinal mucosa epithelial cells shed. LJH-1 was sensitive to cefotaxime and amoxicillin, and resistant to cefradine and amoxicillin. Ant(3″)-Ia, aac(3)-IIa, aph(3')-IIa, Sul2, Sul3 and TEM were detected. This provided scientific data for the detection, prevention and treatment of the bacterial diseases.

Key words: Clostridium cadaveris, identification, pathogenicity, drug resistance

中图分类号:

S852.61

豆朋朋, 魏勇, 王利. 一株羊源尸毒梭菌的鉴定及生物学特性研究[J]. 畜牧兽医学报, 2020, 51(2): 399-403.

DOU Pengpeng, WEI Yong, WANG Li. Identification and Biological Characteristics of Clostridium cadaveris Isolated from Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 399-403.

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一株羊源尸毒梭菌的鉴定及生物学特性研究

Identification and Biological Characteristics of Clostridium cadaveris Isolated from Goat

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