硼对非洲雏鸵鸟胫骨生长的影响

doi:10.11843/j.issn.0366-6964.2013.09.018

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (9): 1462-1467.doi: 10.11843/j.issn.0366-6964.2013.09.018

硼对非洲雏鸵鸟胫骨生长的影响

朱黛云¹，王蕾¹，陈敏^1,2，郑昕婷¹，彭克美^1*

(1. 华中农业大学动物科技-动物医学院动物组织与胚胎实验室，武汉 430070；2. 信阳农林学院，信阳 464000)

收稿日期:2013-03-15 出版日期:2013-09-23 发布日期:2013-09-23
通讯作者: 彭克美，教授，E-mail：kmpeng@sohu.com
作者简介:朱黛云(1988-)，女，内蒙古赤峰人，硕士研究生，主要从事动物组织学与胚胎学的研究，E-mail: zhudaiyun_9876@sina.com，Tel：027-87286970
基金资助:
国家自然科学基金（30471249；31272517）；国家博士点专项科研基金（200805040023）

Effect of Additional Boron on Tibias Osteogenesis of African Ostrich Chicks

ZHU Dai-yun¹, WANG Lei¹, CHEN Min^{1, 2}, ZHENG Xin-ting¹, PENG Ke-mei^1*

(1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; 2. Xinyang Agricultural College, Xinyang 464000, China）

Received:2013-03-15 Online:2013-09-23 Published:2013-09-23

摘要/Abstract

摘要：

本研究旨在探究饮水添加硼对非洲雏鸵鸟胫骨生长发育形态结构的影响。选用16羽雏鸵鸟随机A、B、C、D共4组，每组4只。A、B、C、D 4组分别饮水添加0、100、200、400 mg·L^-1硼30 d。试验结束后，宰杀鸵鸟，选取胫骨骨干上1/4做脱钙骨石蜡切片，光镜下观察各组胫骨的组织结构变化，并进行形态计量学分析。结果表明，与对照组A组(0 mg·L^－1)相比，添加硼的B组(100 mg·L^－1)、C组(200 mg·L^－1)和 D组(400 mg·L^－1)的雏鸵鸟胫骨小梁区域的宽度明显增加，骨髓腔中骨髓细胞增多活跃，骨小梁周长、骨小梁面积、骨小梁相对体积显著增大，其中C组达到最大值；C组骨小梁分离度最小，显著低于A、B组，与D组差异不显著。C组骨小梁的表面成骨细胞和破骨细胞数量最多；D组在钙化的软骨基质区域可见软骨细胞逐渐变成空泡状，出现核缩现象，最后退化死亡。结果提示饮水适量添加硼可以促进雏鸵鸟胫骨形成和骨量的增加。

Abstract:

The aim of the present study was to find out the effects of drinking boron on osteogenesis of tibias of ostrich chicks. Sixteen ostrich chicks were randomly assigned to four groups. The ostrich chicks were fed with 0 (group A), 100 (group B), 200 (group C), and 400 mg·L^－1(group D) of additional boron in water for 30 d. At the end of experiment time, the ostrich chicks were slaughtered and their top quarter of tibias were obtained, then the samples were made into paraffin sections, observed and photographed under Olympus DHP microphotograph. The results showed as follows: 1) The width, the density of trabeculae area and the marrow cavity increased significantly in others three groups, especially in group C, compared with the Group A; 2) Perimeter, area, and relative volume of trabeculae increased significantly and the maximum were occurred in group C. There were a very large number of osteoblasts and osteoclasts on the surface of trabeculae in group C. The trabecular separating degree in Group C was the minimum and significantly lower than in Group A and Group B, but had no significant difference compared with Group D; 3) In Group D, chondrocytes became focally vacuolated gradually in calcified cartilage matrix area, nuclear appeared pyknotic phenomenon, finally degradation and death appeared. These results indicated that the additional boron supplemented in drinking could promote obviously tibias osteogenesis of African Ostrich Chicks, and 200 mg·L^-1 supplement boron in the drinking water appeared to be the most beneficial.

中图分类号:

S852.1

朱黛云，王蕾，陈敏，郑昕婷，彭克美. 硼对非洲雏鸵鸟胫骨生长的影响[J]. 畜牧兽医学报, 2013, 44(9): 1462-1467.

ZHU Dai-yun, WANG Lei, CHEN Min, ZHENG Xin-ting, PENG Ke-mei. Effect of Additional Boron on Tibias Osteogenesis of African Ostrich Chicks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1462-1467.

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硼对非洲雏鸵鸟胫骨生长的影响

Effect of Additional Boron on Tibias Osteogenesis of African Ostrich Chicks

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