XRCC1在卵子和早期胚胎中的定位与功能的初步分析

doi:10.11843/j.issn.0366-6964.2023.05.033

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (5): 2126-2133.doi: 10.11843/j.issn.0366-6964.2023.05.033

XRCC1在卵子和早期胚胎中的定位与功能的初步分析

朱家桥^1,2,3*, 程来洋², 曹江琴², 朱闽², 李军伟², 鞠辉明^2,3, 刘宗平^2,3

1. 扬州大学广陵学院, 扬州 225009;
2. 扬州大学兽医学院, 扬州 225009;
3. 江苏高校动物重要疫病与人畜共患病防控协同创新中心, 扬州 225009

收稿日期:2022-10-14 出版日期:2023-05-23 发布日期:2023-05-20
通讯作者: 朱家桥,E-mail:jqzhu1998@163.com
作者简介:朱家桥(1979-),甘肃兰州人,副教授,主要从事临床兽医和动物生殖发育研究
基金资助:
江苏省自然科学基金面上项目(BK20221284);扬州大学创新基金项目(ZHU005349);江苏高校优势学科建设工程项目(PAPD2018)

Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos

ZHU Jiaqiao^1,2,3*, CHENG Laiyang², CAO Jiangqin², ZHU Min², LI Junwei², JU Huimin^2,3, LIU Zongping^2,3

1. Guangling College of Yangzhou University, Yangzhou 225009, China;
2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

Received:2022-10-14 Online:2023-05-23 Published:2023-05-20

摘要/Abstract

摘要： X射线修复交叉互补蛋白1(XRCC1)是一种支架蛋白,其在碱基切除修复中发挥重要作用。本文检测了XRCC1在卵母细胞和胚胎中的亚细胞定位,并研究了其对小鼠卵母细胞减数分裂成熟的影响,以及对随后的胚胎发育和基因组DNA甲基化的影响。结果显示,在卵母细胞成熟和受精后胚胎发育过程中,XRCC1主要定位在细胞核中。通过显微注射特异性抗体来阻断XRCC1后,卵母细胞生发泡破裂率和胚胎卵裂率出现显著性下降,同时2细胞胚胎中基因组DNA去甲基化水平异常。这说明XRCC1主要定位于细胞周期间期的细胞核,功能正常的XRCC1有助于减数分裂中小鼠卵母细胞质量的维持和早期胚胎的正常发育。

关键词: XRCC1, 小鼠胚胎, DNA去甲基化, 卵母细胞

Abstract: XRCC1 is a molecular scaffold protein that plays a central role in the base excision repair pathway. Here, we examined the subcellular localization of XRCC1 and investigated its effects on meiotic maturation of oocytes, subsequent embryonic development, and global DNA methylation in mice. XRCC1 was found to be localized mainly in the nuclei during oocyte maturation and subsequent embryonic development. Blocking XRCC1 by microinjection with antibodies partly reduced oocyte germinal vesicle breakdown (GVBD) and embryo cleavage, and also disrupts DNA demethylation in the 2-cell embryos. The results indicates that XRCC1 is mainly located in the nuclei at the interphase of cell cycle, and XRCC1 with normal function contributes to the maintenance of the quality of mouse oocytes during meiosis and the normal development of early embryos.

Key words: XRCC1, mouse embryo, DNA demethylation, oocyte

中图分类号:

S852.1

朱家桥, 程来洋, 曹江琴, 朱闽, 李军伟, 鞠辉明, 刘宗平. XRCC1在卵子和早期胚胎中的定位与功能的初步分析[J]. 畜牧兽医学报, 2023, 54(5): 2126-2133.

ZHU Jiaqiao, CHENG Laiyang, CAO Jiangqin, ZHU Min, LI Junwei, JU Huimin, LIU Zongping. Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2126-2133.

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XRCC1在卵子和早期胚胎中的定位与功能的初步分析

Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos

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