幼年牦牛不同部位皮肤毛囊的组织学结构及TGF-β2与HIF-1α差异性表达分析

doi:10.11843/j.issn.0366-6964.2021.011.024

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (11): 3224-3233.doi: 10.11843/j.issn.0366-6964.2021.011.024

幼年牦牛不同部位皮肤毛囊的组织学结构及TGF-β2与HIF-1α差异性表达分析

张虔¹, 崔燕^1,2*, 余四九^1,2, 廖博¹, 邹胜南¹, 白雪峰¹, Seth Yaw Afedo¹, 赵鹏飞¹, 龙敏¹

1. 甘肃农业大学动物医学院, 兰州 730070;
2. 甘肃省牛羊胚胎工程技术研究中心, 兰州 730070

收稿日期:2021-02-14 出版日期:2021-11-23 发布日期:2021-11-24
通讯作者: 崔燕,主要从事动物比较组织学研究,E-mail:cuiyan369@sina.com
作者简介:张虔(1995-),男,甘肃武威人,硕士,主要从事动物组织学与胚胎学研究,E-mail:zq9507@126.com
基金资助:
国家自然科学基金项目（31972634）

Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens)

ZHANG Qian¹, CUI Yan^1,2*, YU Sijiu^1,2, LIAO Bo¹, ZOU Shengnan¹, BAI Xuefeng¹, Seth Yaw Afedo¹, ZHAO Pengfei¹, LONG Min¹

1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2. Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070, China

Received:2021-02-14 Online:2021-11-23 Published:2021-11-24

摘要/Abstract

摘要： 旨在探究幼年牦牛皮肤毛囊的组织学结构和TGF-β2及HIF-1α对幼年牦牛皮肤毛囊生长发育的影响，选取10头健康幼年牦牛，采集其颈部、背部、胸部、腹部、小腿部、腋下及阴囊皮肤组织，制作石蜡切片后，采用HE和Sacpic染色，对各部位皮肤组织中毛囊进行观察和计数，并筛选出多毛及少毛部位。利用qRT-PCR、Western blot及免疫组织化学法对TGF-β2和HIF-1α在幼年牦牛多毛皮肤和少毛皮肤进行定位与定量的初步研究。结果表明，幼年牦牛皮肤的毛囊分布于真皮层，常与汗腺及皮脂腺伴行，毛髓质及内根鞘结构不完整。Sacpic染色可见毛囊内根鞘为红色，外根鞘为苍绿色，结缔组织鞘为蓝绿色。腹部皮肤的毛囊数量最多[（2 085±15）个·cm^-2]，阴囊数量最少[（158±15）个·cm^-2]。免疫组织化学结果显示，TGF-β2及HIF-1α主要表达在表皮层、毛囊外根鞘、皮脂腺及汗腺。TGF-β2在阴囊和腋下的mRNA转录水平和蛋白质表达水平均显著高于腹部和背部。HIF-1α在腹部的mRNA转录水平和蛋白质表达水平均显著高于其他三个部位。幼年牦牛的毛囊处于退行期，在腹部数量最多，背部次之，阴囊最少；TGF-β2和HIF-1α在不同部位皮肤中的表达水平存在显著差异，为进一步研究TGF-β2和HIF-1α对牦牛皮肤毛囊生长发育的影响提供理论依据。

关键词: 幼年牦牛, 多毛皮肤, 少毛皮肤, TGF-β2, HIF-1α

Abstract: This study was conducted to investigate the histological structure and effects of TGF-β2 and HIF-1α on growth and development of hair follicles in young yaks. Ten healthy young yaks, Skin tissues from their neck, back, breast, abdomen, crus, axilla and scrotum were collected. Paraffin sections were prepared from tissues, stained with HE and Sacpic, and then observed under optical microscope after which representative photomicrographs were taken for analysis. Hair follicles were counted in each part of the skin tissue and categorized as either hairy or less hairy skin. Then qRT-PCR, Western blot, and immunohistochemistry techniques were used to preliminary study TGF-β2 and HIF-1α expression and localization in the hairy or less hairy skin of young yaks. The results showed that hair follicles in the skin of young yaks were distributed in the dermis, often accompanied by sweat glands and sebaceous glands. The structure of hair medulla and inner root sheath was incomplete. Sacpic staining showed the inner root sheath of hair follicles as red, the outer root sheath as pale green, and connective tissue sheath as blue-green. The numbers of hair follicles in the abdominal skin and scrotal skin were (2 085±15)·cm^-2 and (158±15)·cm^-2 respectively. Immunohistochemistry results showed that TGF-β2 and HIF-1α were mainly distributed in the epidermis, outer root sheath of hair follicle, sebaceous glands, and sweat glands. The mRNA and protein levels of TGF-β2 in scrotum and axillary were significantly higher than those in abdomen and back. The mRNA and protein levels of HIF-1α in the abdomen were significantly higher than those in the other three sites. In conclusion, young yaks’ hair follicles were in the catagen phase. The abdomen had the largest number of hair follicles, followed by the back, and the scrotum, the least. The expression levels of TGF-β2 and HIF-1α were significantly varied in different parts of the skin, which provided a theoretical basis for further study on the effects of TGF-β2 and HIF-1α on hair follicle growth and development in yak skin.

Key words: young yak, hairy skin, less hairy skin, TGF-β2, HIF-1α

中图分类号:

S852.16

张虔, 崔燕, 余四九, 廖博, 邹胜南, 白雪峰, Seth Yaw Afedo, 赵鹏飞, 龙敏. 幼年牦牛不同部位皮肤毛囊的组织学结构及TGF-β2与HIF-1α差异性表达分析[J]. 畜牧兽医学报, 2021, 52(11): 3224-3233.

ZHANG Qian, CUI Yan, YU Sijiu, LIAO Bo, ZOU Shengnan, BAI Xuefeng, Seth Yaw Afedo, ZHAO Pengfei, LONG Min. Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens)[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3224-3233.

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幼年牦牛不同部位皮肤毛囊的组织学结构及TGF-β2与HIF-1α差异性表达分析

Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens)

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