猪脾转移因子提高LaSota株鸡新城疫弱毒疫苗的免疫保护率

doi:10.11843/j.issn.0366-6964.2021.012.024

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (12): 3578-3587.doi: 10.11843/j.issn.0366-6964.2021.012.024

猪脾转移因子提高LaSota株鸡新城疫弱毒疫苗的免疫保护率

徐磊¹, 钟佳莲¹, 余勋信¹, 刘毅发¹, 黄瑜^2,3*, 刘小龙⁴, 赖隆永⁴, 闫丽萍⁵, 许秀梅⁶, 宋素泉^5*, 张渊魁^6*

1. 福建农业职业技术学院, 福州 350119;
2. 福建省禽病防治重点实验室, 福州 350013;
3. 福建省农业科学院畜牧兽医研究所, 福州 350013;
4. 派生特(福州)生物科技有限公司, 福州 350500;
5. 南京农业大学动物医学院, 南京 210095;
6. 兆丰华生物科技(南京)有限公司, 南京 211102

收稿日期:2021-03-22 出版日期:2021-12-25 发布日期:2021-12-22
通讯作者: 黄瑜,主要从事畜禽传染病研究,E-mail:huangyu_815@163.com;宋素泉,主要从事畜禽传染病研究,E-mail:suquan.song@njau.edu.cn;张渊魁,主要从事畜禽传染病研究,E-mail:Zhang.yuankui@qq.com
作者简介:徐磊(1984-),男,江西赣州人,副教授,主要从事畜禽传染病研究,E-mail:xulei0328@163.com
基金资助:
福建省第七批引才“百人计划”；福建省科技计划项目（2019N0025）；江苏省博士后科研资助计划（2019K072）；福建省高层次人才和青年优秀人才访学研修资助计划（闽人社文〔2019〕239号）；福州市科技计划项目（2020-PT-149）；福建省畜禽疫病防控技术重大研发平台项目（2014N2003）；福建省禽病防治重点实验室开放基金课题（KF201902）；福建省职业院校专业带头人培养计划（闽教办师〔2019〕40号）；福建省科技计划项目（2021S2003）

Improvement of the Immune Protection Rate on Newcastle Disease Virus Attenuated Vaccine LaSota Strain by Pig Spleen Transfer Factor

XU Lei¹, ZHONG Jialian¹, YU Xunxin¹, LIU Yifa¹, HUANG Yu^2,3*, LIU Xiaolong⁴, LAI Longyong⁴, YAN Liping⁵, XU Xiumei⁶, SONG Suquan^5*, ZHANG Yuankui^6*

1. Fujian Agricultural Vocational Technical College, Fuzhou 350119, China;
2. Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou 350013, China;
3. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
4. Present (Fuzhou) Biotech Company Limited, Fuzhou 350500, China;
5. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
6. Zhaofenghua Biotechnology (Nanjing) Co., Ltd., Nanjing 211102, China

Received:2021-03-22 Online:2021-12-25 Published:2021-12-22

摘要/Abstract

摘要： 旨在了解猪脾转移因子（TF）对新城疫病毒（NDV）弱毒疫苗LaSota株的免疫增强效果及机制。本研究分别采用不同剂量（10^-2、10^-3、10^-4、10^-5羽份） LaSota疫苗株单独（单独免疫组）、LaSota疫苗株与TF联合（联合免疫组）免疫SPF鸡，14 d后以NDV F₄₈E₉强毒攻毒，同时设立对照组（非免疫攻毒）和空白组（非免疫非攻毒），并采用ELISA方法与蛋白质芯片技术分别测定外周血IL-4、IFN-γ与IL-12 P40浓度。结果显示，鸡免疫10^-2、10^-3、10^-4、10^-5羽份时疫苗攻毒保护率和半数保护量（PD₅₀）如下：单独免疫组分别为100%、55%、0%、0%和0.000 8羽份，联合免疫组分别为100%、75%、0%、0%和0.000 5羽份，对照组和空白组死亡率分别为100%和0%。免疫10^-3羽份疫苗后，联合免疫组IL-4和IFN-γ含量均高于其他组，并于第7、14天差异极显著（P<0.01）；攻毒后，联合免疫组IL-4、IFN-γ和IL-12 P40含量均高于其他组，IL-4于第1、14天，IFN-γ于第1、3天，IL-12 P40于第1天差异极显著（P<0.01）。综上表明，TF可增强IFN-γ、IL-12介导的细胞免疫和IL-4介导的体液免疫，提高LaSota株弱毒疫苗的免疫保护率，降低疫苗PD₅₀；在抵抗NDV F₄₈E₉强毒攻击时，联合免疫组的免疫保护率明显高于单独免疫组。

关键词: 猪脾转移因子, 鸡新城疫弱毒LaSota株, 鸡新城疫强毒F₄₈E₉株, 免疫保护率, 细胞因子

Abstract: This study was conducted to evaluate the immune enhancement effect and mechanism of pig spleen transfer factor(TF) on Newcastle disease virus(NDV) attenuated vaccine LaSota strain. Specific pathogen free(SPF) chickens were inoculated with different doses(10^-2, 10^-3,10^-4, 10^-5) of NDV strain LaSota(vaccination group without TF), NDV strain LaSota combined with TF(vaccination group with TF), respectively, and challenged with virulent NDV strain F₄₈E₉ on 14 days post vaccination(dpv). The control group(non-immunized but challenged) and blank group(non-immunized and non-challenged) were set up simultaneously. In addition, the change of IL-4, IFN-γ and IL-12 P40 in peripheral blood were analyzed by ELISA and protein chip technology. The protection rate of different doses and median protective dose(PD₅₀) were 100%, 55%, 0%, 0%, 0.000 8 doses in the plume in vaccination group without TF, respectively, and 100%, 75%, 0%, 0%, 0.000 5 doses in the plume in vaccination group with TF. In contrast, the mortality of control group and blank group were 100% and 0%. After immunization with 10^-3 dose of vaccine, the levels of IL-4 and IFN-γ in vaccination group with TF were higher than in other groups and the difference was extremely significant on 7 and 14 dpv(P<0.01). After challenge, the levels of IL-4, IFN-γ and IL-12 P40 in vaccination group with TF were higher than in other groups. The differences of IL-4 on 1 and 14 days post challenge (dpc), IFN-γ on 1 and 3 dpc, IL-12 P40 on 1 dpc were extremely significant(P<0.01). These findings indicated that TF could reinforce cellular immunity by IFN-γ, IL-12 and humoral immunity by IL-4, improve the immune protection rate of NDV attenuated vaccine LaSota strain and reduce the PD₅₀. Vaccination group with TF was more effective than vaccination group without TF against virulent NDV strain F₄₈E₉.

Key words: pig spleen transfer factor, attenuated chicken NDV strain LaSota, virulent chicken NDV strain F₄₈E₉, immune protection rate, cytokine

中图分类号:

S858.315.3

徐磊, 钟佳莲, 余勋信, 刘毅发, 黄瑜, 刘小龙, 赖隆永, 闫丽萍, 许秀梅, 宋素泉, 张渊魁. 猪脾转移因子提高LaSota株鸡新城疫弱毒疫苗的免疫保护率[J]. 畜牧兽医学报, 2021, 52(12): 3578-3587.

XU Lei, ZHONG Jialian, YU Xunxin, LIU Yifa, HUANG Yu, LIU Xiaolong, LAI Longyong, YAN Liping, XU Xiumei, SONG Suquan, ZHANG Yuankui. Improvement of the Immune Protection Rate on Newcastle Disease Virus Attenuated Vaccine LaSota Strain by Pig Spleen Transfer Factor[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3578-3587.

参考文献 26

[1]	XIANG B, CHEN L B, CAI J C, et al. Insights into genomic epidemiology, evolution, and transmission dynamics of genotype VⅡ of class Ⅱ Newcastle disease virus in China[J]. Pathogens, 2020, 9(10):837.
[2]	王静静, 于晓慧, 蒋文明, 等. 2016-2019年我国Ⅰ类新城疫病原学监测与遗传进化分析[J]. 中国动物检疫, 2020, 37(11):1-5.WANG J J, YU X H, JIANG W M, et al. Pathogenic surveillance and phylogenetic analysis on class I newcastle disease virus during 2016 to 2019 in China[J]. China Animal Health Inspection, 2020, 37(11):1-5. (in Chinese)
[3]	ZHU J, HU S L, XU H X, et al. Characterization of virulent Newcastle disease viruses from vaccinated chicken flocks in Eastern China[J]. BMC Vet Res, 2016, 12(1):113.
[4]	丁壮, 金宁一, 王兴龙, 等. 鸡新城疫病毒HN基因亚单位疫苗诱导免疫保护的实验研究[J]. 动物医学进展, 2002, 23(1):49-51.DING Z, JIN N Y, WANG X L, et al. Immunoprotection of chickens from ND by vaccination with expressing HN subunit protein of NDV[J]. Progress in Veterinary Medicine, 2002, 23(1):49-51. (in Chinese)
[5]	刘秀梵, 胡顺林, 刘晓文, 等. 基因Ⅶ型新城疫新型疫苗的创制与应用[J]. 中国畜牧业, 2020(4):24.LIU X F, HU S L, LIU X W, et al. Creation and application of new vaccine for gene ⅤⅡ newcastle disease[J]. China Animal Industry, 2020(4):24. (in Chinese)
[6]	BELLO M B, YUSOFF K, IDERIS A, et al. Diagnostic and vaccination approaches for Newcastle disease virus in poultry:the current and emerging perspectives[J]. Biomed Res Int, 2018, 2018:7278459.
[7]	RAMAEKERS J C. Enhancement of immune response by transfer factor:WO, 2012044960[P]. 2015-04-05.
[8]	VIZA D, FUDENBERG H H, PALARETI A, et al. Transfer factor:an overlooked potential for the prevention and treatment of infectious diseases[J]. Folia Biol (Praha), 2013, 59(2):53-67.
[9]	徐磊, 陈月香, 刘道泉, 等. 猪脾转移因子增强禽白血病病毒A/B亚群抗体阳性鸡群免疫水平的研究[J]. 中国预防兽医学报, 2017, 39(8):649-653.XU L, CHEN Y X, LIU D Q, et al. Study on reinforcing the immune level of ALV-A/B subgroup antibody positive chicken group by pig spleen transfer factor[J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(8):649-653. (in Chinese)
[10]	田澍瑶, 杨雨, 胡玥, 等. 15种免疫增效剂对鸡新城疫免疫效果影响的对比研究[J]. 天津农学院学报, 2020, 27(1):50-52, 57.TIAN S Y, YANG Y, HU Y, et al. Comparative study of effect on immune efficiency to Newcastle disease with fifteen intensifiers[J]. Journal of Tianjin Agricultural University, 2020, 27(1):50-52, 57. (in Chinese)
[11]	徐磊, 陈月香, 刘道泉, 等. 猪脾转移因子增强几种重要禽疫苗免疫效果的测试研究[J]. 中国兽医学报, 2017, 37(10):1944-1950.XU L, CHEN Y X, LIU D Q, et al. Test study on reinforcing immune efficacy of several important avian vaccine by porcine spleen transfer factor[J]. Chinese Journal of Veterinary Science, 2017, 37(10):1944-1950. (in Chinese)
[12]	KRISHNAVENI M. A review on transfer factor an immune modulator[J]. Drug Invent Today, 2013, 5(2):153-156.
[13]	QIU X S, SUN Q, WU S, et al. Entire genome sequence analysis of genotype IX Newcastle disease viruses reveals their early-genotype phylogenetic position and recent-genotype genome size[J]. Virol J, 2011, 8:117.
[14]	徐磊, 刘友霖. 猪疫苗特异性猪脾脏转移因子的制备方法及用途:中国, 201310506686. 7[P]. 2015-05-13.XU L, LIU Y L. Preparation method and application of swine vaccine specific swine spleen transfer factor (TF):CN, 201310506686. 7[P]. 2015-05-13. (in Chinese)
[15]	徐磊. 禽疫苗特异性猪脾脏转移因子的制备方法及用途:中国, 201310507478. 9[P]. 2015-07-08.XU L. Preparation method and use of poultry vaccine-specific pig spleen transfer factor:CN, 201310507478. 9[P]. 2015-07-08. (in Chinese)
[16]	国家市场监督管理总局, 国家标准化管理委员会. GB/T 16550-2020新城疫诊断技术[S]. 北京:中国标准出版社, 2020.State Administration for Market Regulation, Standardization Administration. GB/T 16550-2020 Diagnostic techniques for Newcastle disease[S]. Beijing:China Standard Press, 2020. (in Chinese)
[17]	中国兽药典委员会. 中华人民共和国兽药典(2015年版三部)[M]. 北京:中国农业出版社, 2016.Chinese Veterinary Pharmacopoeia Committee. Chinese veterinary pharmacopoeia (2015 edition part 3)[M]. Beijing:China Agriculture Press, 2016. (in Chinese)
[18]	刘华雷, 王志亮. 新城疫的流行历史与现状[J]. 中国动物检疫, 2015, 32(6):1-4.LIU H L, WANG Z L. The history and current status of Newcastle disease[J]. China Animal Health Inspection, 2015, 32(6):1-4. (in Chinese)
[19]	王友令, 徐怀英, 李莉, 等. 新城疫La Sota疫苗对不同基因型流行株的免疫保护[J]. 浙江农业学报, 2009, 21(5):446-449.WANG Y L, XU H Y, LI L, et al. Immune protection of Newcastle disease La Sota vaccine on different genotypes of Newcastle disease isolates[J]. Acta Agriculturae Zhejiangensis, 2009, 21(5):446-449. (in Chinese)
[20]	胡北侠, 杨少华, 许传田, 等. 新城疫LaSota活疫苗对基因VII型分离株的免疫保护性试验[J]. 广东畜牧兽医科技, 2012, 37(3):34-35.HU B X, YANG S H, XU C T, et al. Immune protection assay of Newcastle disease live vaccine (LaSota Strain) against a genotype VⅡ NDV isolate[J]. Guangdong Journal of Animal and Veterinary Science, 2012, 37(3):34-35. (in Chinese)
[21]	武志伟, 杨雨, 胡玥, 等. 不同动物种类转移因子对家禽免疫效果影响的对比性研究[J]. 黑龙江畜牧兽医, 2020(9):83-84, 88.WU Z W, YANG Y, HU Y, et al. Comparative study of the effect on the immunity to poultry with transfer factors of different animal species[J]. Heilongjiang Animal Science and Veterinary Medicine, 2020(9):83-84, 88. (in Chinese)
[22]	SAHOO A, WALI S, NURIEVA R. T helper 2 and T follicular helper cells:regulation and function of interleukin-4[J]. Cytokine Growth Factor Rev, 2016, 30:29-37.
[23]	SAWANT P M, VERMA P C, SUBUDHI P K, et al. Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-γ and IL-4 genes[J]. Vet Immunol Immunopathol, 2011, 144(1-2):36-44.
[24]	刘琳琳, 宋雨心, 王文骞, 等. 表达鸡IL-12重组乳酸乳球菌的构建及其生物学活性检测[J]. 中国预防兽医学报, 2017, 39(6):495-499.LIU L L, SONG Y X, WANG W Q, et al. Construction of recombinant Lactococcuslactis expressing chicken ChIL-12 and its biological activity identification[J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(6):495-499. (in Chinese)
[25]	GUPTA S, MANUBHAI K P, MUKHERJEE S, et al. Serum profiling for identification of autoantibody signatures in diseases using protein microarrays[M]//GREENING D W, SIMPSON R J. Serum/Plasma Proteomics:Methods and Protocols. New York:Humana Press, 2017:303-315.
[26]	刘温泉. 新城疫F48E9毒株与LaSota毒株感染鸡后外周血部分细胞因子差异表达的研究[D]. 雅安:四川农业大学, 2010:43-44.LIU W Q. The different expression of partial cytokine genes in response to velogenic and lentogenic Newcastle disease viruses infection in chicken peripheral blood[D]. Yaan:Sichuan Agricultural University, 2010:43-44. (in Chinese)

猪脾转移因子提高LaSota株鸡新城疫弱毒疫苗的免疫保护率

Improvement of the Immune Protection Rate on Newcastle Disease Virus Attenuated Vaccine LaSota Strain by Pig Spleen Transfer Factor

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