鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响

doi:10.11843/j.issn.0366-6964.2020.10.025

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (10): 2567-2575.doi: 10.11843/j.issn.0366-6964.2020.10.025

鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响

余祖华¹, 丁轲^1*, 贾艳艳¹, 郁川¹, 何雷¹, 廖成水¹, 李静¹, 张春杰¹, 李银聚¹, 吴庭才¹, 程相朝^1,2, 门凯凯¹, 张瑾¹, 余祖玲¹, 周子誉^1,3, 罗俊^1,3

1. 河南科技大学动物疫病与公共卫生重点实验室, 洛阳市活载体生物材料与动物疫病防控重点实验室, 洛阳 471023;
2. 洛阳职业技术学院, 洛阳 471003;
3. 河南省农业科学院动物免疫学重点实验室, 农业部动物免疫学重点实验室, 河南省动物免疫学重点实验室, 郑州 450002

收稿日期:2020-03-23 出版日期:2020-10-25 发布日期:2020-10-26
通讯作者: 丁轲,主要从事动物分子免疫学研究,E-mail:keding19@163.com
作者简介:余祖华(1977-),女,河南商城人,副教授,博士,主要从事动物分子免疫学研究,E-mail:yzhd05@163.com
基金资助:
国家自然科学基金（31702207，U1504308）；河南科技大学省部级科技创新平台培育项目（2015SPT004）

Effect of Gallus TGFβ1 on the Proliferation, Apoptosis, Migration and Invasion of MDCC-MSB1 Cells

YU Zuhua¹, DING Ke^1*, JIA Yanyan¹, YU Chuan¹, HE Lei¹, LIAO Chengshui¹, LI Jing¹, ZHANG Chunjie¹, LI Yinju¹, WU Tingcai¹, CHENG Xiangchao^1,2, MENG Kaikai¹, ZHANG Jin¹, YU Zuling¹, ZHOU Ziyu^1,3, LUO Jun^1,3

1. Key Laboratory of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang 471023, China;
2. Luoyang Vocational and Technical College, Luoyang 471023, China;
3. Key Laboratory of Animal Immunology of Henan Academy of Agricultural Sciences, Key Laboratory of Animal Immunology of Ministry of Agriculture, Key Laboratory of Animal Immunology in Henan Province, Zhengzhou 450002, China

Received:2020-03-23 Online:2020-10-25 Published:2020-10-26

摘要/Abstract

摘要： 旨在探讨鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响。作者将构建的鸡TGFβ1过表达载体、干扰表达载体以及相应阴性对照转染MDCC-MSB1细胞，然后检测转染后各组细胞鸡TGFβ1的表达水平、细胞增殖能力、细胞周期与凋亡，细胞的迁移与侵袭能力。结果显示，与相应阴性对照相比，转染TGFβ1过表达质粒可显著上调MDCC-MSB1细胞的TGFβ1表达水平，显著抑制MDCC-MSB1细胞的增殖，且使G1期细胞增加、S和G2期细胞减少，同时增加细胞凋亡率，降低细胞的迁移与侵袭能力；转染TGFβ1干扰表达质粒可显著下调MDCC-MSB1细胞的TGFβ1表达水平，显著促进MDCC-MSB1细胞的增殖，G1期细胞减少、S和G2期细胞增加，同时降低细胞凋亡率，增加细胞的迁移与侵袭能力。结果表明，鸡TGFβ1可抑制MDCC-MSB1细胞增殖、迁移与侵袭，促进其凋亡。

关键词: TGFβ1, MDCC-MSB1细胞, 细胞增殖, 细胞凋亡, 迁移, 侵袭

Abstract: This study aimed to detect the effect of Gallus TGFβ1 on the biological behavior of MDCC-MSB1 cells. MDCC-MSB1 cells were transiently transfected with Gallus TGFβ1 overexpression vector, interference expression vector, and the corresponding negative control. Then, the expression of Gallus TGFβ1, the cell proliferation, the cell cycle and apoptosis, the migration and invasion of each transfection groups were examined. Results showed that compared with the corresponding control, the MDCC-MSB1 cells transfected with overexpression vector of Gallus TGFβ1 could up-regulate the expression level of TGFβ1, the proliferation of MDCC-MSB1 cells was significantly inhibited, G1 phase cells were increased, S and G2 cells were decreased, the apoptosis rate of the cells was increased, the migration and invasion ability were decreased.However，the MDCC-MSB1 cells transfected with the interference expression vector of TGFβ1 significantly down-regulated the expression level of TGFβ1, cell proliferation was improved，G1 phase cells were decreased, S and G2 cells were increased, the cell apoptosis was decreased, the migration and invasion ability was increased. The results showed that Gallus TGFβ1 could inhibit the proliferation, migration and invasion of MDCC-MSB1 cells, and promote their apoptosis.

Key words: TGFβ1, MDCC-MSB1 cells, cell proliferation, apoptosis, migration, invasion

中图分类号:

余祖华, 丁轲, 贾艳艳, 郁川, 何雷, 廖成水, 李静, 张春杰, 李银聚, 吴庭才, 程相朝, 门凯凯, 张瑾, 余祖玲, 周子誉, 罗俊. 鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响[J]. 畜牧兽医学报, 2020, 51(10): 2567-2575.

YU Zuhua, DING Ke, JIA Yanyan, YU Chuan, HE Lei, LIAO Chengshui, LI Jing, ZHANG Chunjie, LI Yinju, WU Tingcai, CHENG Xiangchao, MENG Kaikai, ZHANG Jin, YU Zuling, ZHOU Ziyu, LUO Jun. Effect of Gallus TGFβ1 on the Proliferation, Apoptosis, Migration and Invasion of MDCC-MSB1 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2567-2575.

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鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响

Effect of Gallus TGFβ1 on the Proliferation, Apoptosis, Migration and Invasion of MDCC-MSB1 Cells

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