孕酮、干扰素τ对牛子宫内膜上细胞外基质及其相关配体表达的影响

摘要/Abstract

摘要：

为探索干扰素τ（IFN-τ）、孕酮对牛子宫内膜上细胞外基质（ECM）及其配基基因表达的影响，本研究在体外培养的牛子宫内膜细胞（bECs）培养液中，分别添加孕酮、bIFN-τ、孕酮和bIFN-τ混合物培养24 h后，通过荧光定量PCR技术，检测bECs上Collagen、Laminin、Fibronectin、THBS和Tenascin等ECM基因和ECM配基基因Syndecan、CD44、CD47和RHAMM的表达。结果表明：在体外培养的牛子宫内膜细胞上，bIFN-τ显著地诱导了Tenascin、Collagen、RHAMM和Laminin基因的表达，孕酮显著地诱导了CD44、Fibronectin、RHAMM和Laminin基因的表达，但当bIFN-τ和孕酮联合作用时，CD44、THBS基因的转录水平受到抑制，Tenascin、Fibonectin、Collagen基因的转录水平恢复到对照组转录水平。研究结果显示，在胚胎植入过程中，IFN-τ和孕酮可能对牛子宫内膜上ECM及其配基基因表达具有调控作用。

Abstract:

To reveal the effects of interferon τ (IFN-τ) and progesterone on the expression level of extracellular matrix (ECM) and their ligands genes in bovine endometrial cells (bECs). In the study, bECs were cultured in medium containing bovine IFN-τ (bIFN-τ), progesterone and combination of bIFN-τ and progesterone, respectively, and then expression level of ECM related genes (Collagen, laminin, Fibronectin, THBS and Tenascin) and their ligand genes (Syndecan, CD44, CD47 and RHAMM) in bECs were detected by quantitative PCR after in vitro cultured bECs were treated for 24 h with bIFN-τ, progesterone, combination of bIFN-τ and progesterone, respectively. The results showed that, bIFN-τ significantly induce the expression of Tenascin, Collagen, RHAMM and Laminin, and progesterone significantly induced the expression of CD44, Fibronectin, RHAMM, and Laminin, but under co-action of bIFN-τ and progesterone, the expression level of CD44 and THBS was inhibited, and the expression of Tenascin, Fibonectin and Collagen returned to the expression level in the control group. The results suggest that bIFN-τ and progesterone might regulate the expression of ECM and their ligands in the process of embryo implantation.

中图分类号:

S823
S814.8

张明，郑洁，甘潇，陈仕毅，曾长军，周光彬，赖松家. 孕酮、干扰素τ对牛子宫内膜上细胞外基质及其相关配体表达的影响[J]. 畜牧兽医学报, doi: .

ZHANG Ming, ZHENG Jie, GAN Xiao, CHEN Shi-yi, ZENG Chang-jun, ZHOU Guang-bin, LAI Song-jia. Effects of Progesterone，Interferon-τ on Expression of the Related Genes of Extracellular Matrix-Their Ligands in Cultured Bovine Endometrial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2013/V44/I2/309

参考文献

［1］张明. 牛IFN-τ基因成熟蛋白CDS区克隆、原核表达及其在妊娠建立过程中的生物学功能研究［D］.雅安：四川农业大学, 2007.

［2］LESSEY B A. Adhesion molecules and implantation ［J］. J Reprod Immunol, 2002, 55:101-112.

［3］CAMENISCH T D, SPICER A P, BREHM-GIBSON T, et al. Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme［J］ .J Clin Invest, 2000, 106(3): 349-360.

［4］FURNUS C C, DEMATOS D G, MARTINEZ A G. Effect of hyaluronic acid on development of in vitro produced bovine embryos［J］ . Theriogenology,1998, 49(8): 1489-1499.

［5］赵红卫, 陈学进, 李青旺, 等. HA对牛卵母细胞体外成熟和早期胚胎体外发育影响的研究［J］. 西北农林科技大学学报(自然科学版), 2004,6: 912.

［6］STOJKOVIC M, KOLLE S, PEINL S, et al. Effect of high concentration of hyaluronic acid in culture medium increases the survival rate of frozen or thawed in vitro produced bovine embryos produced in vitro［ J］. Reproduction, 2002, 124(7): 141-153.

［7］SCHUPPAN D, SOMASUNDARAM R. The extracellular matrix: a major signal transduction network［M］// In: CLEMENT B, GUILLUZO A. Celluar and molecular aspect of Cirrhosis. Colloque INSERM/John Libbey Eruotext Ltd,1992: 115-134, 216.

［8］EARL U, ESTLIN C, BUMMER J N. Fibronectin and laminin in the early human Placenta［J］. Placenta,1990，11(3)：223-231.

［9］LARSON R C, IGNOTZ G G, CURRIE W B. Effect of fibronectin on early embryo development in cow［J］. Reprood Fertil, 1992,96(1):289-293.

［10］郝晶，高英茂，武玉玲, 等，层粘蛋白和纤维粘连蛋白在着床期小鼠子宫内膜中的表达［J］.解剖学杂志，2001，24(1):28-30.

［11］KAR M, SENGUPTA J, KUMAR S, et al. Immunohistochemical localization of macrophage CD68⁺, HLA-DR⁺, L1⁺ and CD44⁺ subsets in uterine endometrium during different phases of menstrual cycle ［J］. Indian J Physiol Pharmacol, 2004, 48(3):293-303.

［12］YAEGASHI N, FUJITA N, YAJIMA A, et al. Menstrual cycle dependent expression of CD44 in normal human endometrium ［J］. Hum Pathol, 1995, 26(8): 862-865.

［13］LAI T H, KING J A, SHIHLE M, et al. Immunological localization of syndecan-1 in human endometrium throughout the menstrual cycle ［J］. Fertil Steril, 2007, 87(1):121-126.

［14］SEDELE M, KARAVELI S, PESTERELI H E, et al. Tenascin expression in normal, hyperplastic, and neoplastic endometrium ［J］. Int J Gynecol Pathol, 2002, 21(2):161-166.

［15］NODA N, MINOURA H, NISHIURA R, et al. Expression of tenascin-C in stromal cells of the murine uterus during early pregnancy: induction by interleukin-1 alpha, prostaglandin E2, and prostaglandin F-2 alpha ［J］. Biol Reprod, 2000, 63(6): 1713-1720.

［16］TEODORO W R, WITZEL S S, VELOSA A P, et al. Increase of interstitial collagen in the mouse endometrium during decidualization ［J］. Connect Tissue Res, 2003, 44(2): 96-103.

［17］CARBONE K, PINTO N M, ABRAHAMSOHN P A, et al. Arrangement and fine structure of collagen fibrils in the decidualized mouse endometrium ［J］. Microsc Res Technol, 2006, 69(1): 36-45.

［18］SPIESS K, TEODORO W R, ZORN T M. Distribution of collagen types Ⅰ, Ⅲ, and V in pregnant mouse endometrium ［J］. Connect Tissue Res, 2007, 48(2):99-108.

［19］KALOGU C, ONARLIOGLU B. Extracellular matrix remodelling in rat endometrium during early pregnancy: the role of fibronectin and laminin ［J］. Tissue Cell, 2010, 42(5):301-306.

［20］张明，甘潇，郑洁, 等. 孕酮对体外培养的牛子宫内膜细胞上孕酮受体表达的影响［J］. 畜牧兽医学报，2008,39（9）：1190-1195.

［21］FORTIER M A, GUIBAULT L A, GRASSO F. Specific proerties of epithelial and stromal cells from the endometrium of cows［J］. Reprod Fertil, 1988, 83: 239-248.

［22］MATTHEWS C J, REDFERM C P, HIRST B H, et al. Characterization of human purified epithelial and stromal cells from endometrium and endometriosis in tissue culture［J］. Fertil Steril, 1992, 57(5): 990-997.

［23］BRUBAKER D B, ROSS M G, MARINOFF D. The function of elevated plasma fibronectin in preeclampsia［J］. Am J Obstet Gynecol, 1992, 166(2): 526-531.

［24］STAGGS K L, AUSTIN K J, JOHNSON G A, et al. Complex induction of bovine uterine proteins by interferon-tau［J］. Biol Reprod, 1998, 59: 293-297.

［25］AROSH J A, BANU S K, KIMMINS S, et al. Effect of interferon tau on prostaglandin biosynthesis, transport and signaling at the time of maternal recognition of pregnancy in cattle: Evidence of polycrine actions of PGE2［J］. Endocrinology, 2004, 145(11): 5280-5293.

［26］FLORENCE D B, ANTONIS E K. Induction of PG G/H synthase-2 in bovine myometrial cells by interferon-τ requires the activation of the p38 MAPK pathway ［J］.Endocrinology, 2001, 142(12):5107-5115.

［27］GUZELOGU A, BILBY T R, MEIKLE A, et al. Pregnancy and bovine somatotropin in nonlactating dairy cows: Ⅱ. Endometrial gene expression related to maintenance of pregnancy ［J］. Am J Dairy Sci, 2004, 87: 3268-3279.

［28］JAMES K P, RUEDA B R, KATHY J A, et al. Interferontau suppresses prostaglandin F2_α secretion independently of the Mitogen-activated protein kinase and nuclear factor κB pathway ［J］. Biol Reprod, 2001, 64:965-973.

［29］KIYOSHI O, YUKO K, SHUKO M, et al. Interferon-τ block the stimulatory effect of tumor necrosis factor-α on prostaglandin F₂_α synthesis by bovine endometrial stromal cells［J］. Biol Reprod, 2004, 70: 191-197.

［30］PARENT J, CHRISTAN V, ANDREI P A, et al. Influence of different isoforms of recombinant trophoblastic interferon on prostaglandin production in cultured bovine endometrial cells ［J］. Biol Reprod, 2003, 68: 1035-1043.

［31］RALPH T, ROSS A D B, STEFANIE J, et al. Transcription regulation of the bovine oxytocin receptor gene ［J］. Biol Reprod, 2003, 68: 1015-1026.

［32］SUSANNE H, ROSS B, CHRISOSTOMOS L, et al. Bovine endometrial epithelial cells as a model system to study oxytocin receptor regulation ［J］. Hum Reprod Update, 1998, 4(5):605-614.

［33］MULARONI A, MAHFOUDI , BECK L, et al. Progesterone control of fibronectin secretion in Guinea pig endometrium ［J］. Endocrinology,1992,131(5):2127-2130.

［34］HUPPERTZ B, KERTSCHANSKA S, FRANK H G, et al. Extracellular matrix components of the placental extravillous trophblast:immunocyto chemistry and ultrastrual distribution ［J］. Histochem Cell Biol,1996,106:291-294.

[1]	曹建华, 杨柏高, 张培培, 冯肖艺, 张航, 余洲, 牛一凡, 郝海生, 杜卫华, 朱化彬, 杨凌, 赵学明. 能量负平衡影响奶牛卵泡发育的机制[J]. 畜牧兽医学报, 2024, 55(1): 22-30.
[2]	苗舒, 安济山, 王祚, 肖定福, 兰欣怡, 刘磊, 沈维军, 万发春. 亮氨酸通过PI3K-AKT信号通路促进牛成肌细胞的增殖[J]. 畜牧兽医学报, 2024, 55(1): 142-152.
[3]	刘益丽, 唐娇, 闵奇, 杨露, 王泽宁, 胡莲, 赵迪, 江明锋. 基于转录组数据挖掘牦牛皱胃发育代谢的关键候选基因[J]. 畜牧兽医学报, 2024, 55(1): 153-168.
[4]	姚颖, 周应聪, 杜培岩, 李一娟, 钱文洁, 李柳杨, 余志鹏, 崔燕, 余四九, 樊江峰. 基于TMT技术的牦牛妊娠期血清蛋白质组学分析[J]. 畜牧兽医学报, 2024, 55(1): 192-206.
[5]	张颖, 袁莉刚, 陈国娟, 张芳, 杨大鹏. ET-1/eNOS表达差异在牦牛隐睾发生中的作用分析[J]. 畜牧兽医学报, 2024, 55(1): 207-217.
[6]	张文涛, 刘晨阳, 朱炳霖, 柳丽, 田媛, 姚宇航, 成功. Snail1对牛脂肪细胞增殖分化影响及作用机制的研究[J]. 畜牧兽医学报, 2023, 54(12): 5008-5019.
[7]	牛一凡, 杨柏高, 张培培, 张航, 冯肖艺, 曹建华, 余洲, 郝海生, 杜卫华, 邹惠影, 朱化彬, 马友记, 赵学明. 牛胚胎基因组选择研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4449-4457.
[8]	王文翔, 杜丽丽, 胡俊伟, 张琰翔, 马铭昊, 段蕊, 钱聪, 王欣玥, 李三禄, 张长庆, 张路培, 高雪, 徐凌洋, 李俊雅, 高会江. 基于转录组分析挖掘平凉红牛背最长肌肉质性状候选基因[J]. 畜牧兽医学报, 2023, 54(11): 4589-4604.
[9]	张倩, 崔燕, 余四九, 何俊峰, 潘阳阳, 王萌. 初生及成年牦牛大脑皮质的转录组分析[J]. 畜牧兽医学报, 2023, 54(11): 4605-4614.
[10]	董智豪, 时玉新, 郭冠华, 原开敏, 修豪宇, 汪超, 白俊艳, 王栋. 母牛不同发情阶段唾液化合物的比较分析[J]. 畜牧兽医学报, 2023, 54(11): 4636-4652.
[11]	孙东晓, 张胜利, 张勤, 李姣, 张桂香, 刘丑生, 郑伟杰. 我国奶牛基因组选择技术应用进展[J]. 畜牧兽医学报, 2023, 54(10): 4028-4039.
[12]	冯肖艺, 郝海生, 杜卫华, 朱化彬, 崔凯, 赵学明. 能量负平衡导致奶牛繁殖力下降的机制进展[J]. 畜牧兽医学报, 2023, 54(10): 4050-4060.
[13]	马浩然, 张路培, 金生云, 宝金山, 李红艳, 高会江, 徐凌洋, 王泽昭, 李俊雅. 利用高密度SNP芯片评估中国地方肉牛品种基因组亲缘关系[J]. 畜牧兽医学报, 2023, 54(10): 4174-4185.
[14]	郭逸芯, 王之盛, 胡瑞, 王俊梅, 王森, 施丽媛, 张晓红, 邹华围, 左家学, 彭全辉, 薛白, 王立志. 亮氨酸对黄牛皮下脂肪细胞棕色化的影响[J]. 畜牧兽医学报, 2023, 54(8): 3286-3298.
[15]	褚楚, 张静静, 丁磊, 樊懿楷, 包向男, 向世馨, 刘锐, 罗雪路, 任小丽, 李春芳, 刘文举, 王亮, 刘莉, 李永青, 江汉, 李委奇, 孙伟, 李喜和, 温万, 周佳敏, 张淑君. 基于中红外光谱的牛奶中三种氨基酸含量预测模型的建立及应用[J]. 畜牧兽医学报, 2023, 54(8): 3299-3312.