环介导等温扩增技术的发展及其在耐药基因检测中的应用

doi:10.11843/j.issn.0366-6964.2025.04.013

摘要/Abstract

摘要：

本文介绍了环介导等温扩增(loop-mediated isothermal amplification, LAMP)技术的原理、过程和特点，以及其在耐药基因检测中的应用。LAMP技术以其高效、特异、灵敏和简便的特点，在分子生物学领域得到了广泛应用。除了基本的LAMP检测方法外，本文还对一些新型LAMP技术，如IMS-LAMP、LAMP与CRISPR/Cas系统的联用、LAMP与Argonaute系统的联用、LAMP传感器以及LAMP与微流控芯片的结合等做了介绍。针对耐药基因的检测中，LAMP技术展现了很好的应用前景，为快速、准确地检测耐药基因提供了有效的工具。

关键词: 环介导等温扩增, 恒温扩增, 快速扩增, 检测方法, 耐药基因检测

Abstract:

This paper describes the principle, process and characteristics of loop-mediated isothermal amplification (LAMP) technology and its application in the detection of drug resistance genes.LAMP technology has been widely used in the field of molecular biology due to its high efficiency, specificity, sensitivity and simplicity. In addition to the basic LAMP detection methods, some novel LAMP technologies, such as IMS-LAMP, LAMP coupled with CRISPR/Cas system, LAMP coupled with Argonaute system, LAMP sensors, and LAMP coupled with microfluidic chip, are also introduced in this paper. In addition, for the detection of drug-resistant genes, LAMP technology shows a good application prospect in the detection of drug resistance genes, which provides an effective tool for rapid and accurate detection of drug resistance genes.

Key words: loop-mediated isothermal amplification, constant temperature amplification, rapid amplification, detection methods, drug resistance gene detection

中图分类号:

S852.6

廖怡雯, 叶景芬, 武绍碧, 陈世雄, 杨婉, 罗雪, 杨琦. 环介导等温扩增技术的发展及其在耐药基因检测中的应用[J]. 畜牧兽医学报, 2025, 56(4): 1621-1631.

LIAO Yiwen, YE Jingfen, WU Shaobi, CHEN Shixiong, YANG Wan, LUO Xue, YANG Qi. Development of Ring-mediated Isothermal Amplification Technology and Its Application to the Detection of Drug Resistance Genes[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1621-1631.

图/表 2

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新型LAMP New type LAMP	特点 Specificities	应用范围 Scope of application
IMS-LAMP	高度灵敏和特异性，结合免疫磁分离技术进行样品富集和纯化	鼠疫耶尔森菌^[27]、鼠伤寒沙门菌^[28]、大肠杆菌^[28]、金黄色葡萄球菌^[28]
LAMP-CRISPR/Cas	高特异性识别和切割目标序列，结合CRISPR的基因编辑能力增强检测精度	SARS-CoV-2病毒^[34]、人乳头瘤病毒^[35]、脑膜炎双球菌^[36]
LAMP-Argonaute	使用Argonaute蛋白进行靶向识别切割，结合LAMP提高检测灵敏度	人乳头瘤病毒^[41]、FAdV-4^[43]、SARS-CoV-2病毒^[44]
LAMP传感器 LAMP sensors	集成多种传感器技术，实现对扩增产物的实时监测和检测	SARS-CoV-2病毒^[46]、大肠杆菌^[47]、沙门菌^[47]、金黄色葡萄球菌^[47]
LAMP与微流控芯片的结合 Combination of LAMP and microfluidic chips	自动化和集成化处理，减少操作步骤和误差，适用于小样本量和便携应用	寨卡病毒^[49]、登革病毒^[49]、基孔肯亚病毒^[49]、丙型肝炎病毒^[50]、乙型肝炎病毒^[50]、人类免疫缺陷病毒^[50]

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