三株传染性法氏囊病病毒重组毒株的致病性研究

doi:10.11843/j.issn.0366-6964.2024.06.026

摘要/Abstract

摘要：

鸡传染性法氏囊病(IBD)是由传染性法氏囊病毒(IBDV)引起雏鸡发病的一种高致病性免疫抑制性疾病，目前IBDV重组变异株性对鸡养殖业有严重危害。本研究对三株IBDV重组毒株进行全基因序列分析并测定其致病性差异。通过鸡胚分离鉴定并增殖三株IBDV毒株，利用Mega 11、MegAlign软件与参考毒株进行核苷酸同源性比对和氨基酸进化树绘制，将病原感染SPF鸡测定致病性，用实时荧光定量PCR方法检测脏器病毒拷贝量，结果显示：GD-1株属于A2dB3型，呈现新型变异A超强B特点，GD-2株和GD-3株同属于A3B1型，分别呈现超强A新型变异B和超强A早期变异B特点；三株病毒均未引起雏鸡死亡，GD-1株无明显临床症状，GD-2株和GD-3株在攻毒后7 d出现精神沉郁，卧地不起；引起其法氏囊显著萎缩、脾肿大，法氏囊载毒量要高于脾载毒量，在攻毒后第14天时GD-1株的法氏囊载毒量显著高于GD-2株和GD-3株；三组试验鸡法氏囊滤泡均发生萎缩，且GD-2组和GD-3组病变更严重，脾则是GD-1组病变更严重。本研究测定三株IBDV的序列、绘制进化树，测定致病性，显示三株病毒都有新型变异株的变异趋势，为研究IBDV致病机理和防控IBD提供参考。

关键词: 传染性法氏囊病毒, 序列分析, 重组病毒, 致病性

Abstract:

Infectious bursal disease is a highly contagious, immunosuppressive disease of young chickens caused by infectious bursal disease virus (IBDV). In recent years, recombinant IBDV mainly threats to the poultry industry. In our study, we characterized whole-gene sequence analysis of three recombinant strains of infectious bursal disease virus and assessed its pathogenicity in specific pathogen-free chickens. Three strains of virus were identified and amplified by chicken embryo isolation. The nucleotide homology and amino acid evolution tree were compared with the reference strain by Mega 11 and MegAlign software. The pathogen was infected with SPF chickens to determine the pathogenicity, and the viral copies in organs were detected by real-time fluorescence quantitative PCR. Phylogenetic analysis revealed that GD-1 strain belonged to type A2dB3, and showed the characteristics of new variant A, very virulent B; Strain GD-2 and strain GD-3 belong to type A3B1, showed the characteristics of very virulent A, new variant B and very virulent A, early variant B. Pathogenicity assays showed that none of the three strains caused the death of chicks, GD-1 strain had no obvious clinical symptoms, and GD-2 strain and GD-3 strain showed depression and lying on the ground 7 days after the attack. It caused significant atrophy of bursa of Fabricius and enlargement of spleen. The load of toxin in bursa of Fabricius was higher than that in spleen. On the day 14 after challenge, the bursa of bursa of GD-1 strain was significantly higher than that of GD-2 strain and GD-3 strain. Three groups of experimental chickens bursa of fabricius follicles are shrinking, and GD-2 group and GD-3 pathological changes is more serious, the spleen is a GD-1 set of pathological changes is more serious. In this study, the sequence of the three strains was determined, the evolutionary tree was drawn, and the pathogenicity was determined. It may help to study the pathogenic mechanism and the prevention of infectious bursal virus in poultry.

Key words: infectious bursal disease virus, sequence analysis, recombinant virus, pathogenicity

中图分类号:

S852.659.4

杨坤, 马静文, 周新瑞, 罗烈柱, 刘喆, 胡自强, 武星辰, 梁立滨, 高诗敏. 三株传染性法氏囊病病毒重组毒株的致病性研究[J]. 畜牧兽医学报, 2024, 55(6): 2550-2559.

Kun YANG, Jingwen MA, Xinrui ZHOU, Liezhu LUO, Zhe LIU, Ziqiang HU, Xingchen WU, Libin LIANG, Shimin GAO. Pathogenicity of Three Recombinant Strains of Infectious Bursal Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2550-2559.

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毒株名称 Strain name	A节段序列号 A segment serial number	B节段序列号 B segment serial number	来源 Source	毒力类型 Virulence type
HK46	AF092943	AF092944	中国香港	vvIBDV
02015.1	AJ879932	AJ880090	法国	vvIBDV
OKYM	D49706	D49707	日本	vvIBDV
D6948	AF240686	AF240687	荷兰	vvIBDV
Gx	AY444873	AY705393	中国	vvIBDV
Harbin-1	EF517528	EF517529	中国	vvIBDV
UK661	X92760	AJ318897	英国	vvIBDV
MB	DQ927040	DQ927041	中国	vvIBDV
HLJ-0504	GQ451330	GQ451331	中国	vvIBDV
YS07	FJ695138	FJ695139	中国	vvIBDV
NN1172	MW245064	MW245065	中国	vvIBDV
Cu-lwt	AF362747	AF362748	法国	cIBDV
Faragher 52-70	HG974565	HG974566	法国	cIBDV
TL2004	DQ088175	DQ118374	中国	attIBDV
CEF94	AF194428	AF194429	荷兰	attIBDV
Gt	DQ403248	DQ403249	中国	attIBDV
CE	EU162089	EU162092	中国	attIBDV
B87	DQ906921	DQ906922	中国	attIBDV
GLS	AY368653	AY368654	美国	vIBDV
Variant-E	AF133904	AF133905	美国	vIBDV
9109	AY462027	AY459321	美国	vIBDV
SHG19	MN393076	MN393077	中国	NvIBDV
SHG352	MT179720	MT179722	中国	NvIBDV
SHG358	MT179721	MT179723	中国	NvIBDV
OH	IBU30818	IBU30819	加拿大	Serotype Ⅱ
2382	AF362773	AF362774	德国	Serotype Ⅱ

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