畜禽肌纤维发育相关细胞种类及鉴定方法的研究进展

doi:10.11843/j.issn.0366-6964.2024.12.001

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (12): 5325-5339.doi: 10.11843/j.issn.0366-6964.2024.12.001

畜禽肌纤维发育相关细胞种类及鉴定方法的研究进展

吴丹妮¹(), 谢遇春⁵, 秦箐¹, 张崇妍¹, 徐晓龙¹, 赵丹¹, 兰茗熙¹, 杨继⁴, 徐松松⁴, 刘志红¹^,²^,³^,*()

1. 内蒙古农业大学, 呼和浩特 010018
2. 农业农村部肉羊遗传育种重点实验室, 呼和浩特 010018
3. 内蒙古自治区羊遗传育种与繁殖重点实验室, 呼和浩特 010018
4. 中国农业大学, 北京 100193
5. 河北科技师范学院, 秦皇岛 066004

收稿日期:2024-05-08 出版日期:2024-12-23 发布日期:2024-12-27
通讯作者: 刘志红 E-mail:2604879241@qq.com;Liuzh7799@163.com
作者简介:吴丹妮(2000-)，女，内蒙古巴彦淖尔人，硕士，主要从事动物遗传育种研究，E-mail: 2604879241@qq.com
基金资助:
国家重点研发计划(2021YFD1200901);国家自然科学基金地区项目(32060742);北方农牧业技术创新中心项目(BFGJ2022002);内蒙古自治区科技重大专项(2020ZD0004);内蒙古自治区高等学校青年科技英才支持项目(NJYT23012);中央引导地方科技发展资金(2022ZY0185);内蒙古自治区直属高校基本科研业务费项目科研创新平台能力建设专项(BR221005);内蒙古自治区教育厅一流学科科研专项项目(YLXKZX-NND-007);内蒙古自治区科技计划(2023KYPT0021);新疆动物生物技术重点实验室开放课题资助课题(2023SWS004)

Research Progress on Cell Types and Identification Methods Related to Muscle Fiber Development in Livestock and Poultry

WU Danni¹(), XIE Yuchun⁵, QIN Qing¹, ZHANG Chongyan¹, XU Xiaolong¹, ZHAO Dan¹, LAN Mingxi¹, YANG Ji⁴, XU Songsong⁴, LIU Zhihong¹^,²^,³^,*()

1. Inner Mongolia Agricultural University, Hohhot 010018, China
2. Key Laboratory of Mutton Sheep & Goat Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
3. Inner Mongolia Key Laboratory of Sheep & Goat Genetics Breeding and Reproduction, Hohhot 010018, China
4. China Agricultural University, Beijing 100193, China
5. Hebei Normal University of Science & Technology, Qinhuangdao 066004, China

Received:2024-05-08 Online:2024-12-23 Published:2024-12-27
Contact: LIU Zhihong E-mail:2604879241@qq.com;Liuzh7799@163.com

摘要/Abstract

摘要：

肌细胞(又名肌纤维) 是组成骨骼肌的基本单位, 约占畜禽机体的50% 左右, 与畜禽产肉性能、骨骼保护密切相关, 对调节畜禽机体代谢和内分泌有重要作用, 同时也为畜禽的运动提供了动力。骨骼肌是一种较为复杂的组织, 而组成这些组织的基本单位是肌细胞, 骨骼肌在发育时期需要多种类型的单核和多核细胞相互作用, 这是形成优质肉类的关键。近些年, 随着科学技术的快速发展, 对鉴定骨骼肌发育相关细胞有了更多且全面的技术, 而单细胞测序技术在其中运用的最为广泛, 它的运用对组成骨骼肌的细胞类型和相关细胞发育异质性的研究提供了技术支持, 对肉品质的研究有重大意义。本文综述了通过细胞形态学观察、分子标记物、免疫组织化学、免疫荧光、原位杂交、单细胞测序的方法鉴定与骨骼肌发育过程相关的细胞, 归纳与骨骼肌发育相关细胞的标记基因, 为了解骨骼肌的结构和功能, 为探索骨骼肌发育机制提供参考, 为肉品质的研究提供理论基础, 也在不同品种的个体选育中具有重要意义和实践价值。

关键词: 骨骼肌, 骨骼肌发育, 细胞鉴定

Abstract:

Muscle cells (also known as muscle fibers) are the basic unit of skeletal muscle, accounting for about 50% of the body of livestock and poultry, and are closely related to meat production performance and bone protection of livestock and poultry. They play an important role in regulating the metabolism and endocrine of livestock and poultry, and also provide power for the movement of livestock and poultry. Skeletal muscle is a relatively complex tissue, and the basic unit of these tissues are muscle cells. Skeletal muscle requires a variety of types of mononuclear and multinucleated cells to interact during development, which is the key to the formation of high-quality meat. In recent years, with the rapid development of science and technology, there are more and more comprehensive techniques for identifying skeletal muscle development-related cells, and single cell sequencing technology is most widely used. Its application provides technical support for the study of cell types and related cell development heterogeneity of skeletal muscle, and is of great significance for the study of meat quality. This paper reviews the identification of cells related to skeletal muscle development by cell morphology observation, molecular markers, immunohistochemistry, immunofluorescence, in situ hybridization, and single cell sequencing, and summarizes the marker genes of cells related to skeletal muscle development, in order to understand the structure and function of skeletal muscle. It provides a reference for exploring the mechanism of skeletal muscle development, provides a theoretical basis for the study of meat quality, and also has important significance and practical value in individual breeding of different varieties.

Key words: skeletal muscle, skeletal muscle development, cell identification

中图分类号:

S852.2

吴丹妮, 谢遇春, 秦箐, 张崇妍, 徐晓龙, 赵丹, 兰茗熙, 杨继, 徐松松, 刘志红. 畜禽肌纤维发育相关细胞种类及鉴定方法的研究进展[J]. 畜牧兽医学报, 2024, 55(12): 5325-5339.

WU Danni, XIE Yuchun, QIN Qing, ZHANG Chongyan, XU Xiaolong, ZHAO Dan, LAN Mingxi, YANG Ji, XU Songsong, LIU Zhihong. Research Progress on Cell Types and Identification Methods Related to Muscle Fiber Development in Livestock and Poultry[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5325-5339.

图/表 5

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动物 Animal	肌肉部位 Muscle types	时期 Period	不同发育时期细胞类型变化情况 Changes of cell types at different developmental stages	采用的技术 Used technology	主要细胞类型 Main cell types	参考文献 Reference
牛 Cattle	背最长肌	胚胎期(怀孕后60 d)、哺乳期(出生后4月龄)和成年期(出生后24月龄)	胚胎期成纤维细胞占比最多；哺乳期内皮细胞占比最多，其次是IIX型肌纤维；成年期IIX型肌纤维占比最多	10×Genomics单细胞RNA测序	18个主要细胞类型：脂肪细胞、内皮细胞、红系细胞、成纤维细胞、淋巴细胞、单核细胞、成肌细胞、肌细胞、肌源性祖细胞、中性粒细胞、周细胞、雪旺氏细胞、腱细胞、Ⅰ型纤维1、Ⅰ型纤维2、IIA型纤维1、IA型纤维2和IIX型纤维	[102]
猪 Pig	背最长肌	1日龄新生猪	/	10×Genomics单细胞RNA测序	11种不同的细胞类群，包括内皮细胞(24.39%)、肌管(18.82%)、纤维成脂祖细胞(18.11%)、卫星细胞(16.74%)、成肌细胞(3.99%)、肌细胞(5.74%)、雪旺细胞(3.81%)、平滑肌细胞(3.22%)、树突状细胞(2.99%)、周细胞(1.86%)和中性粒细胞(0.33%)	[66]
鸡 Chicken	胸肌	生长早期(孵化后第5天)和快速生长期(第100天)	在生长早期鉴定到了5个成肌细胞群和2个脂肪细胞群，在快速生长期鉴定到了1个成肌细胞群和脂肪细胞群	10×Genomics单细胞RNA测序	生长早期(孵化后第5天)鉴定出4种细胞类型：成肌细胞、脂肪细胞、红细胞和其他细胞。快速生长期(第100天)鉴定出6种细胞类型：成肌细胞、脂肪细胞、干细胞、红细胞、内皮细胞和其他细胞	[103]

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畜禽肌纤维发育相关细胞种类及鉴定方法的研究进展

Research Progress on Cell Types and Identification Methods Related to Muscle Fiber Development in Livestock and Poultry

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