基于小鼠骨骼肌损伤模型分析丹参素对骨骼肌损伤后修复再生的促进作用

doi:10.11843/j.issn.0366-6964.2023.12.034

摘要/Abstract

摘要： 旨在探明丹参单体成分丹参素在骨骼肌损伤后修复中的作用,以期为临床治疗提供理论依据。本试验建立骨骼肌损伤模型,设置丹参素治疗组,通过HE染色和Masson染色观察肌纤维修复过程,Western blot和Q-PCR方法检测肌再生相关因子(α-Actinin、eMyHC、MyoD、MyoG、Pax7和MSTN),炎症相关因子(IL-6和IL-10),巨噬细胞类型相关因子(F4/80),瘢痕组织形成相关因子(Collagen Ⅰ和α-SMA)及肌间脂肪相关因子PPAR-γ的表达。结果显示,丹参素降低损伤组腓肠肌对Evans blue的摄取,显著增加α-Actinin和eMyHC因子的表达水平。与损伤组相比,肌肉再生相关因子MyoD、MyoG和Pax7的表达水平在丹参素治疗之后显著升高,而MSTN显著减少。丹参素显著降低损伤组F4/80的表达,加快M1型巨噬细胞向M2型巨噬细胞的转化,降低IL-6 mRNA水平,增加IL-10 mRNA水平。Masson染色结果显示,丹参素减少受损骨骼肌胶原纤维的分布,并且降低Collagen Ⅰ和α-SMA的表达。同时,丹参素显著增加肌间脂肪因子PPAR-γ的表达。因此,丹参素能够加快肌纤维的再生,促进肌间脂肪的形成,减少纤维化瘢痕的生成,从而加快骨骼肌修复进程,提高骨骼肌修复质量。

关键词: 骨骼肌损伤与再生, 丹参素, 肌间脂肪, 炎症反应, 纤维化

Abstract: The purpose of this study was to investigate the effect of Danshensu, the monomer component of Salvia miltiorrhiza, on the repair of skeletal muscle injury, in order to provide the theoretical basis for clinical treatment. The skeletal muscle injury models were established and treated with Danshensu. The process of skeletal muscle repair was observed via H.E. and Masson staining. Western blot and Q-PCR were used to detect the expression of muscle regeneration related factors (α-Actinin, eMyHC, MyoD, MyoG, Pax7 and MSTN), inflammation related factors (IL-6 and IL-10), macrophage type related factors (F4/80), scar tissue formation related factors (Collagen Ⅰ and α-SMA) and intermuscular adipose-related factors (PPAR-γ). The results showed that Danshensu reduced Evans Blue uptake of injure gastrocnemius muscle and significantly increased the expression levels of α-Actinin and eMyHC. Meanwhile, the expression levels of muscle regeneration related factors, MyoD, MyoG and Pax7, were also significantly increased and MSTN was obviously decreased after adding Danshensu. Danshansu significantly reduced the expression of F4/80 and accelerated the transformation of M1 macrophages into M2 macrophages, reducing the IL-6 mRNA level and increasing the IL-10 mRNA level. Masson staining showed that Danshensu reduced the distribution of collagen fibers. In addition, it also reduced the expression of Collagen I and α-SMA. Meanwhile, Danshensu significantly increased the expression of PPAR-γ in muscle. Therefore, Danshensu can promote muscle fiber regeneration and the formation of intermuscular adipose, reduce the formation of scar tissue, thereby accelerating the process of skeletal muscle repair and improving the quality of skeletal muscle repair.

Key words: skeletal muscle injury and regeneration, Danshensu, intermuscular adipose, inflammatory response, fibrosis

中图分类号:

薛霖莉, 孙睿, 郝晓静, 曹校瑞, 王海东, 卢嘉茵. 基于小鼠骨骼肌损伤模型分析丹参素对骨骼肌损伤后修复再生的促进作用[J]. 畜牧兽医学报, 2023, 54(12): 5252-5263.

XUE Linli, SUN Rui, HAO Xiaojing, CAO Xiaorui, WANG Haidong, LU Jiayin. The Promoting Effect Analysis of Danshensu on Skeletal Muscle Repair and Regeneration after Skeletal Muscle Injury in Mice based on a Mouse Skeletal Muscle Injury Model[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5252-5263.

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