鸡脾转移因子对鸡肠道抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2017.10.023

摘要/Abstract

摘要：

旨在通过分析鸡脾转移因子（TF）对鸡肠道抗氧化能力的影响，探讨其调控鸡生长、免疫的作用机制。选用1日龄SPF鸡100羽，自5日龄开始在饮水中加入鸡脾转移因子，饲喂1周，停喂1周，饲喂分为4个剂量组，分别为0.05（最低剂量组）、0.10（低剂量组）、0.25（中剂量组）、1 mL·只^-1（高剂量组）剂量组及正常对照组，饲养至12日龄（连续用药1周）及19日龄（停喂1周）时各取50羽，分别称量其体重，并测量十二指肠、空肠、回肠和直肠的抗氧化能力。结果表明，口服鸡脾转移因子1周，就显示出升高体重的趋势，但是效果尚不明显。高剂量组可显著增加12日龄雏鸡十二指肠、空肠、回肠、直肠SOD（17.07%~31.54%）、CAT（16.98%~25.77%）、GSH-PX（13.99%~23.59%）的活力，以及总抗氧化能力T-AOC（16.48%~43.16%），降低各段MDA含量（P<0.05）；中剂量组显著增加各肠段的T-AOC和SOD活性、CAT活力（回肠除外）、GSH-PX活力（十二指肠除外）；且低、中剂量组均显著降低各肠段的MDA含量（P<0.05）。停喂一周后，尽管各剂量组对各肠段T-AOC、CAT、GSH-PX、SOD、MDA活性的改变较12日龄有不同程度的降低，但是某些肠段较对照组仍然作用显著（P<0.05）。结果提示，鸡脾转移因子作为新型饲料添加剂能显著提高鸡肠道抗氧化应激能力。

Abstract:

The experiment was conducted to study the effect of chicken spleen transfer factor (TF)on intestine antioxidant capacity and the mechanism on production and immunity ability of chicken. One hundred chickens of 1-day-old were selected and were divided into 5 groups. Four TF groups including 20 chickens each received TF treatment as follows: 0.05 mL (Minimum dose group), 0.10 mL (Low dose group), 0.25 mL (Medium dose group), 1 mL (High dose group) per chicken TF were given for one week by drinking water every day from 5-days-old to 12-days-old, respectively, and then fed without TF to 19-days-old. Group 5th was normal control group. Fifty chickens were killed at 12-days-old and 19-days-old, respectively. The chickens were weighted, and the antioxidant capacity of the duodenum, jejunum, ileum and rectum were measured. The results showed that one week' treatment of TF showed an increased tendency of body weight, yet the effect was not obvious. However, the high TF dose group significantly increased the activity of SOD (17.07%-31.54%), CAT (16.98%-25.77%), GSH-PX (13.99%-23.59%) and T-AOC (16.48%-43.16%) of duodenum, jejunum, ileum and rectum, but significantly reduced the activity of MDA (P<0.05) in intestine. The activities of T-AOC, SOD in the intestinal segments, CAT (except for ileum) and GSH-PX (except for duodenum) were significantly increased in the middle dose groups (P<0.05), but the content of MDA was decreased significantly in the low and middle dose groups (P<0.05). At 19 days old, the changes of T-AOC, CAT, GSH-PX, SOD and MDA in each intestinal group were decreased compared with 12 days old, but there was still significant in individual intestinal segment (P<0.05). The results indicate that the TF, as a new feed additive, has a significant role in improving anti-oxidative stress ability.

中图分类号:

S852.1

胡君宜, 李晶, 卢嘉茵, 王子旭, 陈耀星, 黄迪海, 秦卓明, 马保臣, 董玉兰. 鸡脾转移因子对鸡肠道抗氧化能力的影响[J]. 畜牧兽医学报, 2017, 48(10): 1990-1997.

HU Jun-yi, LI Jing, LU Jia-yin, WANG Zi-xu, CHEN Yao-xing, HUANG Di-hai, QIN Zhuo-ming, MA Bao-chen, DONG Yu-lan. The Influence of Chicken Spleen Transfer Factor on Antioxidant Capacity of Chicken Intestine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1990-1997.

参考文献

[1] FARD S H, TOGHYANI M, TABEIDIAN S A. Effect of oyster mushroom wastes on performance, immune responses and intestinal morphology of broiler chickens[J]. Int J Recycl Org Waste Agric, 2014, 3(4): 141-146.
[2] PLACHA I, TAKACOVA J, RYZNER M, et al. Effect of thyme essential oil and selenium on intestine integrity and antioxidant status of broilers[J]. Br Poult Sci, 2014, 55(1): 105-114.
[3] ZHANG X H, SUN Z Y, CAO F L, et al. Effects of dietary supplementation with fermented ginkgo leaves on antioxidant capacity, intestinal morphology and microbial ecology in broiler chicks[J]. Bri Poult Sci, 2015, 56(3): 370-380.
[4] 黄迪海, 刘霞, 盛晓丹, 等. 兽用鸡脾转移因子的多肽组成研究[J]. 中国兽药杂志, 2014, 48(2): 23-27.
HUANG D H, LIU X, SHENG X D, et al. The diverse polypeptides consisting of the chicken spleen transfer factor in veterinary[J]. Chinese Journal of Veterinary Drug, 2014, 48(2): 23-27. (in Chinese)
[5] ALVERDY J C. Effects of glutamine-supplemented diets on immunology of the gut[J]. JPEN J Parenter Enteral Nutr, 1990, 14(4S): 109S-113S.
[6] REIMUND J M, HIRTH C, KOEHL C, et al. Antioxidant and immune status in active Crohn's disease. A possible relationship[J]. Clin Nutr, 2000, 19(1): 43-48.
[7] PIECHOTA-POLANCZYK A, FICHNA J. Review article: the role of oxidative stress in pathogenesis and treatment of inflammatory bowel diseases[J]. Naunyn Schmiedebergs Arch Pharmacol, 2014, 387(7): 605-620.
[8] OGNIK K, CHOLEWI?SKA E, SEMBRATOWICZ I, et al. The potential of using plant antioxidants to stimulate antioxidant mechanisms in poultry[J]. World's Poult Sci J, 2016, 72(2): 291-298.
[9] SALAMI S A, MAJOKA M A, SAHA S, et al. Efficacy of dietary antioxidants on broiler oxidative stress, performance and meat quality: science and market[J]. Avian Biol Res, 2015, 8(2): 65-78.
[10] LAPOINTE J, KIMMINS S, MACLAREN L A, et al. Estrogen selectively up-regulates the phospholipid hydroperoxide glutathione peroxidase in the oviducts[J]. Endocrinology, 2005, 146(6): 2583-2592.
[11] YU B P. Cellular defenses against damage from reactive oxygen species[J]. Physiol Rev, 1994, 74(1): 139-162.
[12] DMITRIEV L F, TITOV V N. Lipid peroxidation in relation to ageing and the role of endogenous aldehydes in diabetes and other age-related diseases[J]. Ageing Res Rev, 2010, 9(2): 200-210.
[13] 于虎, 马瑞珏, 张朔杨, 等. 胸腺素β₄调节血管内皮生长因子、层粘连蛋白5表达, 促进糖尿病大鼠皮肤损伤愈合[J]. 中国糖尿病杂志, 2011, 19(1): 60-63.
YU H, MA R J, ZHANG S Y, et al. Thymosin beta 4 regulates the expression of VEGF and laminin-5 in accelerating skin wound healing in diabetic rat[J]. Chinese Journal of Diabetes, 2011, 19(1): 60-63. (in Chinese)
[14] BANNINK A, DIJKSTRA J, KOOPMANS S J, et al. Physiology, regulation and multifunctional activity of the gut wall: a rationale for multicompartmental modelling[J]. Nutr Res Rev, 2006, 19(2): 227-253.
[15] 汤涌, 黄进. 胸腺素β₄在心脏保护和修复中的作用[J]. 医学综述, 2013, 19(3): 392-394.
TANG Y, HUANG J. Effects of thymosin beta4 in cardiac protection and repair[J]. Medical Recapitulate, 2013, 19(3): 392-394. (in Chinese)
[16] MA J Y, RUAN L W, XU X, et al. Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection[J]. Acta Oceanol Sin, 2016, 35(4): 44-50.
[17] SCHILLACI D, SPINELLO A, CUSIMANO M G, et al. A peptide from human β thymosin as a platform for the development of new anti-biofilm agents for Staphylococcus spp. and Pseudomonas aeruginosa[J]. World J Microbiol Biotechnol, 2016, 32(8): 124.
[18] 孙全友, 徐彬, 王琳燚, 等. 抗菌肽和姜黄素对肉仔鸡生长性能、抗氧化功能及肠道微生物的影响[J]. 中国家禽, 2015, 37(20): 32-36.
SUN Q Y, XU B, WANG L Y, et al. Effects of antimicrobial peptide and curcumin on growth performance, antioxidant performance and intestinal microflora of broilers[J]. China Poultry, 2015, 37(20): 32-36. (in Chinese)
[19] 刘小龙, 乔家运, 范寰, 等. 抗菌肽、合生素对AA肉鸡生长性能和免疫器官指数的影响[J]. 中国饲料, 2016(4): 15-18.
LIU X L, QIAO J Y, FAN H, et al. Effects of dietary antimicrobial peptides and synbiotics on growth performance and immune organ index in AA broiler chickens[J]. China Feed, 2016(4): 15-18. (in Chinese)
[20] 洪育蒲. 热休克蛋白70在急性胰腺炎中的作用[J]. 海南医学, 2016, 27(11): 1823-1826.
HONG Y P. Research progress of heat shock protein 70 in acute pancreatitis[J]. Hainan Medical Journal, 2016, 27(11): 1823-1826. (in Chinese)
[21] DIMAURO I, MERCATELLI N, CAPOROSSI D. Exercise-induced ROS in heat shock proteins response[J]. Free Rad Biol Med, 2016, 98: 46-55.
[22] 郭璐璐, 贾超, 曲彦, 等. 热休克蛋白70对缺血/缺氧嗜铬细胞瘤细胞细胞膜钙通道的调节机制[J]. 中华危重病急救医学, 2016, 28(4): 314-318.
GUO L L, JIA C, QU Y, et al. The modulatory effects of heat shock protein 70 on membrane calcium homeostasis of PC12 cells induced by ischemic/hypoxia[J]. Chinese Critical Care Medicine, 2016, 28(4): 314-318. (in Chinese)
[23] KOPEC W, WILICZKIEWICZ A, JAMROZ D, et al. Antioxidant status of turkey breast meat and blood after feeding a diet enriched with histidine[J]. Poult Sci, 2016, 95(1): 53-61.
[24] 李文立, 路静, 孙振钧, 等. 谷氨酰胺对热应激肉鸡抗氧化性能的影响[J]. 动物营养学报, 2011, 23(4): 695-702.
LI W L, LU J, SUN Z J, et al. Effects of glutamine on antioxidant function of broilers under heat stress[J]. Chinese Journal of Animal Nutrition, 2011, 23(4): 695-702. (in Chinese)
[25] ALMERO J M. Influence of Toll-like receptor 2 and interleukin 10 on the intestinal epithelial barrier and their roles in inflammatory bowel disease[J]. Inmunologia, 2011, 30(1): 8-16.