L-精氨酸对IUGR仔猪胰岛结构及功能的调控研究

doi:10.11843/j.issn.0366-6964.2013.11.012

摘要/Abstract

摘要：

本试验旨在研究L-精氨酸（L-Arg）对子宫内发育迟缓（IUGR）仔猪胰腺生长发育的调控。试验选取12头IUGR仔猪和6头正常仔猪，哺乳至7 d时断奶，将IUGR仔猪随机分为2组，分别饲喂基础人工乳（IUGR组）、基础人工乳+0.6% Arg（IUGR+Arg组），正常仔猪饲喂基础人工乳（NBW组）。饲喂至14 d时每组选取4头屠宰取样，并对胰腺发育相关指标进行测定。结果表明：受IUGR影响，仔猪胰腺组织中胰岛素（Ins）含量、胰岛面积及β细胞质量均显著降低（P<0.05），胰岛细胞数目、Ins染色阳性率、Ins阳性表达面积及Ins阳性表达细胞数目均极显著减少（P<0.01）。IUGR猪补充Arg后，胰腺绝对重量显著增加（P<0.05）；Ins含量、胰岛面积、胰岛细胞数目、β细胞质量、Ins染色阳性率、Ins阳性表达面积也明显增加（P<0.01），且均与NBW猪相比差异不显著（P>0.05）；胰岛平均染色光密度明显增加（P<0.01），且显著高于NBW猪（P<0.01）。结果提示，IUGR损害仔猪胰腺发育，补充Arg后，IUGR仔猪胰岛结构得到改善，胰岛β细胞数量增加，胰岛素合成增多。

Abstract:

This study was conducted to study the regulation effects of L-Arg on the growth and development of pancreas in intrauterine growth retardation (IUGR) piglets. Twelve IUGR piglets and six normal birth weight (NBW) piglets (Duroc×Landrace×Yorkshire) were selected. All piglets were weaned at 7 days of age. Then the IUGR piglets were divided into two groups: IUGR group and IUGR+Arg group, which were supplied with based artificial milk and based artificial milk supplementation with 0.6% Arg, respectively. NBW group was supplied with based artificial milk. Four piglets in each group were slaughtered and sampled at 14 days of age, and the indexes about development of pancreas were measured. The results showed as follows: Pancreatic insulin content, islet area and β-cell mass of IUGR piglets were lower than those of NBW piglets (P<0.05). IUGR significantly reduced the number of islet cells, insulin-positive cell fraction, insulin-positive area and the number of insulin-positive cells (P<0.01). Compared with IUGR control piglets, supplementation with Arg increased body weight and absolute pancreas weight of IUGR piglets (P<0.05), and enhanced pancreatic insulin content, islet area and number of islet cells, as well as β-cell mass, insulin-positive cell fraction and insulin-positive area(P<0.01), which were no significant differences with NBW piglets(P>0.05).The average optical density of IUGR piglets increased by Arg supplementation (P<0.01) and were much higher than that of NBW piglets (P<0.01). These results indicated that IUGR impaired the development of pancreas. L-Arg improved the pancreatic islet structure of IUGR piglets, and increased β-cell mass and insulin synthesis.

中图分类号:

S828
S815.4

孔令蕊，王远孝，李伟，董丽，张婧菲，王恬. L-精氨酸对IUGR仔猪胰岛结构及功能的调控研究[J]. 畜牧兽医学报, 2013, 44(11): 1781-1789.

KONG Ling-rui, WANG Yuan-xiao, LI Wei, DONG Li, ZHANG Jing-fei, WANG Tian. Effects of L-Arg Supplementation on Pancreatic Islet Structure and Function in Intrauterine Growth Retardation Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1781-1789.

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