冷应激对东北野猪成纤维细胞HSP90 mRNA转录水平的影响

畜牧兽医学报

冷应激对东北野猪成纤维细胞HSP90 mRNA转录水平的影响

李忠秋^1，2，刘春龙³，马红²，付博²，汪亮²，彭福刚²，马建章^1*，刘娣^2*

(1.东北林业大学野生动物资源学院，哈尔滨 150040； 2.黑龙江省农业科学院畜牧研究所，哈尔滨 150086；3.中国科学院东北地理与农业生态研究所黑土生态重点实验室，哈尔滨 150081)

收稿日期:2012-03-23 出版日期:2012-12-26 发布日期:2012-12-26
通讯作者: 马建章，教授，院士，E-mail：jianzhangma@163.com；刘娣，教授，E-mail：liudi1963@163.com
作者简介:李忠秋（1974-），女，黑龙江宾县人，助理研究员，博士生，Tel：0451-87502330，E-mail：lizhongqiu1974@163.com；刘春龙（1976-），副研究员，博士。二者并列为第一作者
基金资助:
转基因生物新品种培育重大专项(2008ZX08006-003)；国家自然科学基金(31101700)

Influence of Cold Stress on Transcription of HSP90 in Northeast Wild Boar Fibroblasts

LI Zhong-qiu^1,2, LIU Chun-long³, MA Hong², FU Bo², WANG Liang², PENG Fu-gang², MA Jian-zhang^1*, LIU Di^2*

(1.College of Wildlife Resource, Northeast Forestry University,Harbin 150040,China; 2.Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 3. Key Laboratory of Black Soil Ecology of Heilongjiang Province, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081, China)

Received:2012-03-23 Online:2012-12-26 Published:2012-12-26

摘要/Abstract

摘要：

为了揭示热休克蛋白90基因（Heat shock protein 90，HSP90）在细胞冷应激反应过程中的时空表达特性。以东北野猪成纤维细胞为研究对象，采用实时荧光定量PCR 技术分析了4、15、25和32 ℃不同强度冷应激条件下细胞内HSP90 mRNA转录变化规律。结果表明，在低温处理过程中，HSP90 mRNA转录量在各温度均未见显著增加（P>0.05）；在冷应激的复温培养过程中，HSP90 mRNA转录量在4和15 ℃处理4 h后复温培养8 h内显著增加（P<0.05），峰值出现在6 h，在25和32 ℃处理4 h后复温培养8 h内均未见显著增加；细胞在4和15 ℃处理2、4、6和8 h后，复温培养4 h，HSP90 mRNA转录量随处理温度的降低和时间的延长而增加，在25和32 ℃处理2、4、6和8 h后，复温培养未能显著诱导HSP90 mRNA的转录。结果提示，冷应激诱导东北野猪成纤维细胞内HSP90 mRNA转录量的增加,不是发生在低温处理的应激阶段，而是发生在复温后的细胞应激阶段，温和冷应激（25～32 ℃）未能诱导复温后HSP90 mRNA转录量的显著增加，强度冷应激（4～15 ℃）诱导复温后HSP90 mRNA转录量的显著增加，且与冷应激的强度和时间成正比。

Abstract:

To investigate the spatial and temporal expression profiles of HSP90 gene in fibroblasts of the Northeast Wild boar under cold stress. Real-time fluorescent quantification reverse transcriptase PCR (FQ-RT-PCR) was applied to analyze the expression of HSP90 mRNA in the Northeast Wild boar fibroblasts under 4,15,25 and 32 ℃. The results showed that HSP90 mRNA transcription level didn’t significantly increase (P>0.05) under cold stress treatments at different temperatures; During rewarming culture, HSP90 mRNA transcription levels increased significantly (P<0.05) within 8 h of rewarming incubation following preincubation at 4 or 15 ℃ and the peak showed at the 6^th hour. While HSP90 mRNA transcription levels didn’t increase significantly in the 25 or 32 ℃ preincubation groups within 8 h of rewarming incubation; HSP90 mRNA transcription levels increased with decreasing of the temperature and duration of the cold treatment time when cells were preincubated at 4 or 15 ℃ for 2, 4, 6 or 8 h followed by a 4 h rewarming incubation. However, rewarming incubation didn’t significantly induce transcription of HSP90 mRNA in cells preincubated at 25 or 32 ℃ for 2, 4, 6 or 8 h. The result indicated that cold stress induced increase of HSP90 mRNA transcription level in fibroblasts of the Northeast Wild boar，did not occur in the stress period during low temperature treatment, but in the cellular stress period after rewarming. Mild cold stress (25-32 ℃) didn’t induce a significant increase at HSP90 mRNA transcript levels，while harsh cold stress (4-15 ℃) induced a significant increase at HSP90 mRNA transcript levels which were directly proportional to the intensity and duration of cold exposure at 4-15 ℃.

中图分类号:

S828
S815.4

李忠秋，刘春龙，马红，付博，汪亮，彭福刚，马建章，刘娣. 冷应激对东北野猪成纤维细胞HSP90 mRNA转录水平的影响[J]. 畜牧兽医学报, doi: .

LI Zhong-qiu, LIU Chun-long, MA Hong, FU Bo, WANG Liang, PENG Fu-gang, MA Jian-zhang, LIU Di. Influence of Cold Stress on Transcription of HSP90 in Northeast Wild Boar Fibroblasts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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