中国长三角地区猪繁殖与呼吸综合征病毒流行性Meta分析

doi:10.11843/j.issn.0366-6964.2025.11.037

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (11): 5801-5816.doi: 10.11843/j.issn.0366-6964.2025.11.037

中国长三角地区猪繁殖与呼吸综合征病毒流行性Meta分析

吴含玉(), 夏欣悦(), 徐义刚, 宋厚辉, 王静*(), 杨永春*()

浙江农林大学动物科技学院·动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室, 杭州 311300

收稿日期:2025-01-15 出版日期:2025-11-23 发布日期:2025-11-27
通讯作者: 王静,杨永春 E-mail:502268789@qq.com;359027475@qq.com;jingwang@zafu.edu.cn;yyc@zafu.edu.cn
作者简介:吴含玉(2004-)，女，浙江金华人，本科生，主要从事病毒的分子流行病学及宿主免疫逃逸机制研究，E-mail: 502268789@qq.com
夏欣悦(2001-)，男，浙江金华人，硕士生，主要从事动物病原生物学研究，E-mail: 359027475@qq.com
第一联系人：
吴含玉和夏欣悦是同等贡献作者
基金资助:
国家自然科学基金项目(NSFC 32273017)；浙江省自然科学基金重点项目(LZ23C180001)；浙江农林大学青年教师教改专项(2013110069)；教育部学位与研究生教育发展中心2023年主题案例项目(ZT-231034112)

Meta-analysis of Porcine Reproductive and Respiratory Syndrome Virus Prevalence in China's Yangtze River Delta Region

WU Hanyu(), XIA Xinyue(), XU Yigang, SONG Houhui, WANG Jing*(), YANG Yongchun*()

Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China

Received:2025-01-15 Online:2025-11-23 Published:2025-11-27
Contact: WANG Jing, YANG Yongchun E-mail:502268789@qq.com;359027475@qq.com;jingwang@zafu.edu.cn;yyc@zafu.edu.cn

摘要/Abstract

摘要：

本研究旨在系统评价和分析猪繁殖与呼吸综合征病毒(PRRSV)在我国长三角地区的感染状况，为有效防控PRRSV感染提供科学依据。通过检索中国知网(CNKI)、万方数据知识服务平台、维普中文科技期刊数据库、PubMed和Science Direct等5个主要数据库，收集自1995年PRRSV在中国爆发以来关于长三角区域猪群PRRSV流行情况的文献，并进行系统评价和Meta分析。经过筛选，共纳入36篇文献，样本总数为28 767头猪，其中8 277头为PRRSV阳性。Meta分析结果显示，长三角地区猪群中PRRSV的整体流行率为35.8%(95% CI：0.266, 0.456)。安徽省流行率最高，为51.5%(95%CI：0.321, 0.706)，其次为江苏省45.0%(95%CI：0.315, 0.589)和上海市41.2%(95%CI：0.211, 0.629)，而浙江省的流行率最低，为31.0%(95%CI：0.213, 0.416)。哺乳仔猪的流行率最高，为34.1%(95%CI: 0.181, 0.521)，保育猪为30.4% (95%CI: 0.077, 0.598)，育肥猪则最低，为21.5%(95%CI: 0.115, 0.333)。亚组分析和Meta回归分析表明，文献的异质性来源不包括采样地点、采样时间、诊断方法、临床症状、猪的发育阶段和文献质量(P>0.05)，而样本类型、饲养模式和健康状况等因素均为影响PRRSV流行率的因素(P < 0.05)。我国长三角地区猪群中PRRSV流行率较高，需持续监测其感染情况，特别是对散养猪群和哺乳仔猪的管理和监测，同时制定针对性的防控措施，降低病毒传播风险。

关键词: 中国长三角地区, 猪繁殖与呼吸综合征病毒, Meta分析, 流行性

Abstract:

This study aims to systematically evaluate and analyze the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) in pig populations within the Yangtze River Delta region of China, providing a scientific basis for effective prevention and control strategies. A comprehensive literature search was conducted across five major databases: China National Knowledge Infrastructure (CNKI), Wanfang Data, VIP Chinese Science and Technology Journals Database, PubMed, and Science Direct. Studies on the prevalence of PRRSV in pig populations in the Yangtze River Delta region, collected since the outbreak of PRRSV in China in 1995, was systematically reviewed and subjected to Meta-analysis. A total of 36 articles were included in the Meta-analysis, involving 28 767 pigs, of which 8 277 tested positive for PRRSV. The overall prevalence of PRRSV in pig populations in the Yangtze River Delta region was 35.8% (95% CI: 0.266, 0.456). Among the provinces, Anhui exhibited the highest prevalence at 51.5% (95% CI: 0.321, 0.706), followed by Jiangsu at 45.0% (95% CI: 0.315, 0.589) and Shanghai at 41.2% (95% CI: 0.211, 0.629). Zhejiang had the lowest prevalence at 31.0% (95% CI: 0.213, 0.416). The highest infection rate was observed in suckling piglets (34.1%; 95% CI: 0.181, 0.521), followed by nursery pigs (30.4%; 95% CI: 0.077, 0.598), and finishing pigs (21.5%; 95% CI: 0.115, 0.333). The subgroup analysis and meta-regression analysis revealed that the heterogeneity in the studies was not associated with sampling location, sampling time, diagnostic methods, clinical symptoms, pig developmental stage, or study quality (P>0.05). However, factors such as sample type, feeding practices, and health status were found to significantly affect the prevalence of PRRSV (P < 0.05). The prevalence of PRRSV in pig populations within the Yangtze River Delta region was high. It is essential to maintain continuous monitoring of PRRSV infections, especially the free-range pig and suckling piglets. Additionally, the development and implementation of targeted prevention and control strategies is critical for reducing the risk of viral transmission.

Key words: Yangtze River Delta region, porcine reproductive and respiratory syndrome virus, Meta-analysis, prevalence

中图分类号:

S851.3

吴含玉, 夏欣悦, 徐义刚, 宋厚辉, 王静, 杨永春. 中国长三角地区猪繁殖与呼吸综合征病毒流行性Meta分析[J]. 畜牧兽医学报, 2025, 56(11): 5801-5816.

WU Hanyu, XIA Xinyue, XU Yigang, SONG Houhui, WANG Jing, YANG Yongchun. Meta-analysis of Porcine Reproductive and Respiratory Syndrome Virus Prevalence in China's Yangtze River Delta Region[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5801-5816.

图/表 7

表 1

图 1

表 2

纳入文献基本情况及质量评分"

研究文献 Study ID	采样时间 Sampling time	采样地(省/市) Region (Province/City)	PRRSV抗原和核酸检测 Detection of PRRSV-Ag and RNA			质量评分 Quality score	文献质量 Study quality
研究文献 Study ID	采样时间 Sampling time	采样地(省/市) Region (Province/City)	诊断方法 Diagnose method	阳性样本/总样本 Positive/total samples	检出率/% (95% CI) Detection rate (95% CI)	质量评分 Quality score	文献质量 Study quality
Han et al, 2019^[15]	2016—2017	浙江、江苏 Zhejiang, Jiangsu	ELISA	85/88	0.97 (0.90, 0.99)	4	中等
Jiang et al, 2010^[16]	2006—2007	浙江 Zhejiang	RT-PCR	22/76	0.29 (0.20.0.40)	6	中等
Li et al, 2024^[17]	2021—2022	浙江、江苏 Zhejiang, Jiangsu	RT-qPCR	147/511	0.29 (0.25, 0.33)	7	高
Peng et al, 2017^[18]	2012—2015	长三角地区 Yangtze River Delta	RT-PCR	1 543/2 424	0.64 (0.62.0.66)	6	中等
Rao et al, 2014^[19]	2009—2012	浙江 Zhejiang	RT-qPCR	10/60	0.17 (0.09, 0.28)	6	中等
Wang et al, 2022^[20]	2020	安徽、江苏 Anhui, Jiangsu	RT-PCR	2/13	0.15 (0.04.0.42)	2	低
Wang et al, 2017^[21]	2004—2012	上海 Shanghai	RT-PCR	518/1 499	0.35 (0.32, 0.37)	6	中等
Wang et al, 2015^[22]	2011—2012	上海、江苏 Shanghai, Jiangsu	RT-PCR	5/52	0.10 (0.04, 0.21)	6	中等
Zhou et al, 2022^[23]	2017—2022	浙江、安徽、江苏 Zhejiang, Anhui, Jiangsu	RT-PCR	52/215	0.24 (0.19, 0.30)	8	高
Zhou et al, 2024^[24]	2021—2022	浙江 Zhejiang	MLPA	78/88	0.89 (0.80, 0.94)	4	中等
柏坤桃等, 2008^[25] Bo et al, 2008^[25]	2003—2008	江苏 Jiangsu	RT-PCR	120/372	0.32 (0.28, 0.37)	6	中等
杜庆川, 2024^[26] Du et al, 2024^[26]	2020—2022	江苏 Jiangsu	RT-PCR	1 850/5 620	0.33 (0.32.0.34)	6	中等
顾秋成, 2017^[27] Gu et al, 2017^[27]	2014—2015	安徽 Anhui	RT-PCR	44/211	0.21 (0.16, 0.27)	4	中等
顾小根等, 2002^[28] Gu et al, 2002^[28]	1998—2001	浙江 Zhejiang	ELISA、病毒分离	82/654	0.13 (0.10.0.15)	5	中等
金一春等, 2021^[29] Jin et al, 2021^[29]	2016—2020	上海 Shanghai	RT-PCR	3/5 728	0.00 (0.00.0.00)	6	中等
鞠厚斌等, 2009^[30] Ju et al, 2009^[30]	2006—2009	上海 Shanghai	RT-qPCR	444/1 050	0.25 (0.23, 0.27)	6	中等
李凌光等, 2022^[31] Li et al, 2022^[31]	2019—2022	长三角区域 Yangtze River Delta	RT-PCR	95/277	0.34 (0.29.0.40)	9	高
李玉峰等, 2003^[32] Li et al, 2003^[32]	2001	上海 Shanghai	RT-PCR	18/18	1.00 (0.82, 1.00)	3	低
刘爱军等, 2019^[33] Liu et al, 2019^[33]	2018	浙江 Zhejiang	RT-PCR	8/137	0.06 (0.03, 0.11)	6	中等
刘茂军等, 2009^[34] Liu et al, 2009^[34]	2009	江苏 Jiangsu	RT-PCR	9/21	0.43 (0.24.0.63)	4	中等
刘瑞琳, 2024^[35] Liu et al, 2024^[35]	2021—2023	江苏、上海、浙江 Jiangsu, Shanghai, Zhejiang	RT-PCR	81/285	0.28 (0.23, 0.34)	7	高
刘晓东等, 2014^[36] Liu et al, 2014^[36]	2012	浙江 Zhejiang	RT-PCR	121/247	0.49 (0.43, 0.55)	5	中等
钱琨等, 2011^[37] Qian et al, 2011^[37]	2007—2010	江苏 Jiangsu	RT-PCR	18/45	0.40 (0.27.0.55)	4	中等
沈艳等, 2021^[38] Shen et al, 2021^[38]	2016—2020	安徽 Anhui,	RT-qPCR	200/1 189	0.17 (0.15, 0.19)	9	高
童武等, 2011^[39] Tong et al, 2011^[39]	2010	江苏、上海、浙江 Jiangsu, Shanghai, Zhejiang	RT-PCR	24/65	0.37 (0.26.0.49)	4	中等
王建等, 2015^[40] Wang et al, 2015^[40]	2004—2012	上海 Shanghai	RT-PCR	641/1 629	0.39(0.37.0.42)	9	高
王倩等, 2019^[41] Wang et al, 2019^[41]	2015—2019	安徽 Anhui,	RT-qPCR	375/1 391	0.27 (0.25, 0.29)	5	中等
王强等, 2010^[42] Wang et al, 2010^[42]	2001—2004	安徽 Anhui,	RT-PCR	326/625	0.52 (0.48, 0.56)	8	高
吴瑕等, 2021^[43] Wu et al, 2021^[43]	2017—2020	江苏、上海、浙江 Jiangsu, Shanghai, Zhejiang	RT-PCR	179/357	0.50 (0.45, 0.55)	5	中等
徐丽华等, 2022^[41] Xu et al, 2022^[41]	2017—2020	浙江 Zhejiang	RT-PCR	450/1 963	0.23 (0.21, 0.25)	6	中等
徐丽华等, 2013^[44] Xu et al, 2013^[44]	2004—2009	浙江 Zhejiang	RT-PCR	61/109	0.56 (0.47.0.65)	7	高
叶俊平等, 2007^[45] Ye et al, 2007^[45]	2005—2006	江苏 Jiangsu	RT-PCR	108/211	0.51 (0.44, 0.58)	7	高
叶向阳等, 1999^[46] Ye et al, 1999^[46]	1999	浙江、上海 Zhejiang, Shanghai	ELISA	67/240	0.28 (0.23.0.34)	3	低
张维谊等, 2008^[47] Zhang et al, 2008^[47]	2005—2007	上海 Shanghai	RT-qPCR	240/695	0.35 (0.15, 0.31)	6	中等
赵永旺等, 2010^[48] Zhao et al, 2010^[48]	未知	江苏 Jiangsu	ELISA	18/186	0.10 (0.31, 0.38)	6	中等
祝闰琦等, 2020^[49] Zhu et al, 2020^[49]	2017—2018	安徽 Anhui	RT-qPCR	223/416	0.56 (0.51, 0.61)	8	高

表 2

图 2

图 3

图 4

表 3

长三角地区中的PRRSV流行风险因子相关性分析"

风险因子 Risk factors	文献数量 No.studies	样本总数 No.tested	阳性数目 Positive	流行率/% (CI 95%)	异质性 Heterogeneity			Meta回归分析 Meta-regression
风险因子 Risk factors	文献数量 No.studies	样本总数 No.tested	阳性数目 Positive	流行率/% (CI 95%)	χ²	P-value	I²	P-value	Cofficient(95% CI)	R²/%	T²
采样地点 Region								0.117	-0.051(-0.116, 0.013)	3.56%	0.041
安徽 Anhui	8	3 473	1 517	51.5(0.321, 0.706)	755.35	0.000	99.07%
江苏 Jiangsu	14	2 262	1 029	45.0(0.315, 0.589)	483.56	0.000	97.31%
上海 Shanghai	14	13 795	3 515	41.2(0.211, 0.629)	6 782.46	0.000	99.81%
浙江 Zhejiang	14	3 520	967	31.0(0.213, 0.416)	363.43	0.000	96.42%
方法 Method								0.502	0.042(-0.083, 0.166)	-1.94%	0.039
ELISA	4	1 168	252	36.6(0.092, 0.699)	342.05	0.000	99.12%
RT-PCR	26	23 310	6 771	35.6(0.239, 0.483)	8 460.11	0.000	99.70%
RT-qPCR	5	4 201	1 176	26.2(0.170, 0.360)	185.86	0.000	97.84%
MLPA	1	88	78	88.6(0.80, 0.94)	NA	NA	NA
样本类型 Sample type								0.045^*	0.155(-0.004, 0.306)	19.82%	0.019
血清 Serum	8	7 420	469	21.9(0.054, 0.451)	1 472.20	0.000	99.53%
组织 Tissue	15	7 070	2 143	37.9(0.304, 0.456)	564.68	0.000	97.52%
采样时间 Sampling time								0.596	-0.031(-0.147, 0.086)	-3.41%	0.040
1995—2004	4	1 537	493	48.6(0.217, 0.759)	309.24	0.000	99.03%
2005—2014	15	8 394	3 808	37.5(0.23, 0.47)	603.65	0.000	97.68%
2015—2024	15	18 278	3 838	33.5(0.193, 0.495)	5 709.35	0.000	99.76%
养殖模式 Breeding mode								0.033^*	0.203(0.018, 0.388)	18.57%	0.021
规模化 Scale farming	23	20 217	4 214	25.5(0.194, 0.404)	5 478.19	0.000	99.60%
散养 Free-range farming	4	755	346	44.6(0.336, 0.559)	24.025	0.000	87.51%
发育阶段 Development stage								0.516	-0.038(-0.156, 0.081)	-5.10%	0.043
哺乳仔猪 Lactating piglets	7	2 192	357	34.1(0.181, 0.521)	197.00	0.000	96.95%
保育猪 Nursery pig	5	2 241	410	30.4(0.077, 0.598)	463.11	0.000	99.14%
育肥猪 Fattening pigs	12	6 070	959	21.5(0.115, 0.333)	999.36	0.000	98.90%
健康状况 Health status								0.005^*	-0.240(-0.403, -0.078)	22.40%	0.031
无症状 Asymptomatic	7	9 133	722	11.4(0.013, 0.292)	1 863.27	0.000	99.68%
有症状 Symptomatic	26	51 055	31 943	38.1(0.233, 0.541)	19 994.23	0.000	99.88%
临床症状 Clinical symptoms								0.346	0.214(-0.274, 0.703)	0.32%	0.091
繁殖障碍 Reproductive disorders	7	2 369	296	31.3(0.110, 0.562)	545.87	0.000	98.90%
呼吸道疾病 Respiratory diseases	4	369	214	56.2(0.165, 0.919)	106.86	0.000	97.19%
文献质量 Literature quality								0.419	0.051(-0.075, 0.177)	-1.80%	0.039
高 High	10	5 467	1 944	38.1(0.29, 0.48)	443.09	0.000	97.97%
中 Medium	23	24 005	6 315	32.2(0.21, 0.45)	8 117.41	0.000	99.73%
低 Low	3	271	87	35.3(0.04, 0.98)	NA	NA

表 3

参考文献 57

1	AN T Q , LI J N , SU C M , et al. Molecular and cellular mechanisms for PRRSV pathogenesis and host response to infection[J]. Virus Res, 2020, 286, 197980. doi: 10.1016/j.virusres.2020.197980
2	蔡雪辉, 安同庆. 猪蓝耳病的前世今生[J]. 中国科学: 生命科学, 2023, 53 (12): 1745- 1753.
	CAI X H , AN T Q . Porcine reproductive and respiratory syndrome: past and present[J]. Scientia Sinica(Vitae), 2023, 53 (12): 1745- 1753.
3	赵世伟. 高致病性猪蓝耳病的临床诊断与防治措施[J]. 中国畜牧业, 2022 (11): 96- 97.
	ZHAO S W . Clinical diagnosis and preventive measures of highly pathogenic porcine blue ear disease[J]. China Animal Industry, 2022 (11): 96- 97.
4	刘爱军, 张传亮, 黄晓兵, 等. 猪繁殖与呼吸综合征病毒生命周期的研究进展[J]. 畜牧兽医学报, 2025, 56 (3): 1027- 1041. doi: 10.11843/j.issn.0366-6964.2025.03.006
	LIU A J , ZHANG C L , HUANG X B , et al. Research progress on the life cycle of porcine reproductive and respiratory syndrome virus[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56 (3): 1027- 1041. doi: 10.11843/j.issn.0366-6964.2025.03.006
5	DU T , NAN Y , XIAO S , et al. Antiviral strategies against PRRSV infection[J]. Trends Microbiol, 2017, 25 (12): 968- 979. doi: 10.1016/j.tim.2017.06.001
6	郭宝清, 陈章水, 刘文兴, 等. 从疑似PRRS流产胎儿分离PRRSV的研究[J]. 中国畜禽传染病, 1996 (2): 3- 7.
	GUO B Q , CHEN Z S , LIU W X , et al. Isolation of PRRSV from aborted foetuses suspected of PRRS[J]. Chinese Journal of Preventive Veterinary Medicine, 1996 (2): 3- 7.
7	郑杰, 宁宜宝, 赵启祖, 等. 高致病性变异株引起的猪繁殖与呼吸综合征在我国的发生与流行[J]. 中国兽医杂志, 2007 (6): 48- 50.
	ZHENG J , NING Y B , ZHAO Q Z , et al. Occurrence and prevalence of porcine reproductive and respiratory syndrome caused by highly pathogenic mutant strains in China[J]. Chinese Journal of Veterinary Medicine, 2007 (6): 48- 50.
8	SUN Q , XU H , AN T , et al. Recent progress in studies of porcine reproductive and respiratory syndrome virus 1 in China[J]. Viruses, 2023, 15 (7): 1528. doi: 10.3390/v15071528
9	王丽丽. 浅谈猪蓝耳病的防治措施[J]. 现代畜牧科技, 2021 (1): 80- 82.
	WANG L L . Control measures for blue ear disease in pig[J]. Modern Animal Husbandry Science & Technology, 2021 (1): 80- 82.
10	何克文. 基层养猪常见猪病的预防与治疗[J]. 国外畜牧学(猪与禽), 2021, 41 (4): 36- 37.
	HE K W . Prevention and treatment of common pig diseases in grassroots pig farming[J]. Animal Science Abroad (Pigs and Poultry), 2021, 41 (4): 36- 37.
11	MATHEW J L . Systematic reviews and Meta-analysis: a guide for beginners[J]. Indian Pediatr, 2022, 59 (4): 320- 330. doi: 10.1007/s13312-022-2500-y
12	高辉, 方敏, 姜玲玲, 等. 中国2012-2022年羊群中蓝舌病毒流行的Meta分析[J]. 畜牧兽医学报, 2024, 55 (2): 706- 717. doi: 10.11843/j.issn.0366-6964.2024.02.028
	GAO H , FANG M , JIANG L L , et al. Meta-analysis of bluetongue virus prevalence in sheep flocks in China from 2012-2022[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55 (2): 706- 717. doi: 10.11843/j.issn.0366-6964.2024.02.028
13	申远航, 黄晓灵, 高利伟, 等. 中草药对断奶仔猪生长性能影响的Meta分析[J]. 畜牧兽医学报, 2020, 51 (4): 861- 872. doi: 10.11843/j.issn.0366-6964.2020.04.023
	SHEN W H , HUANG X L , GAO L W , et al. Effect of Chinese herbal medicine on the growth performance of weaned piglets by meta-analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51 (4): 861- 872. doi: 10.11843/j.issn.0366-6964.2020.04.023
14	沈鹏, 王艺, 任炜杰, 等. 牛结节性皮肤病免疫抗体监测的Meta分析[J]. 畜牧兽医学报, 2024, 55 (8): 3649- 3658. doi: 10.11843/j.issn.0366-6964.2024.08.036
	SHEN P , WANG Y , REN W J , et al. Meta analysis of immune antibody monitoring for lumpy skin disease[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55 (8): 3649- 3658. doi: 10.11843/j.issn.0366-6964.2024.08.036
15	HAN G , XU H , WANG K , et al. Emergence of two different recombinant PRRSV strains with low neutralizing antibody susceptibility in China[J]. Sci Rep, 2019, 9 (1): 2490. doi: 10.1038/s41598-019-39059-8
16	JIANG Y , SHANG H , XU H , et al. Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction[J]. Vet J, 2010, 183 (2): 172- 175. doi: 10.1016/j.tvjl.2008.11.016
17	LI C , FAN A , LIU Z , et al. Prevalence, time of infection, and diversity of porcine reproductive and respiratory syndrome virus in china[J]. Viruses, 2024, 16 (5): 774. doi: 10.3390/v16050774
18	PENG Z , ZHAO T , LIANG W , et al. RT-PCR detection of porcine reproductive and respiratory syndrome virus based on the ORF5 gene in Chinese mainland, 2012-2015[J]. Acta Virol, 2017, 61 (3): 336- 340. doi: 10.4149/av_2017_312
19	RAO P , WU H , JIANG Y , et al. Development of an EvaGreen-based multiplex real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of six viral pathogens of porcine reproductive and respiratory disorder[J]. J Virol Methods, 2014, 208, 56- 62. doi: 10.1016/j.jviromet.2014.06.027
20	WANG J , ZHU X , CAI C , et al. Risk analysis of viral diseases in infected pig farms during the lockdown period in China, January to May 2020[J]. Int J Environ Res Public Health, 2022, 19 (6): 3215. doi: 10.3390/ijerph19063215
21	WANG L J , XIE W , CHEN X X , et al. Molecular epidemiology of porcine reproductive and respiratory syndrome virus in central China since 2014: The prevalence of NADC30-like PRRSVs[J]. Microb Pathog, 2017, 109, 20- 28. doi: 10.1016/j.micpath.2017.05.021
22	WANG S , ZHANG M , LIU X C , et al. Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China[J]. J Integr Agric, 2015, 14 (9): 1838- 1844. doi: 10.1016/S2095-3119(15)61044-9
23	ZHOU L , YANG Y , XIA Q , et al. Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017-2022[J]. Front Microbiol, 2022, 13, 971817. doi: 10.3389/fmicb.2022.971817
24	ZHOU Y , YU H , ZHAO X , et al. Detection and differentiation of seven porcine respiratory pathogens using a multiplex ligation-dependent probe amplification assay[J]. Vet J, 2024, 305, 106124. doi: 10.1016/j.tvjl.2024.106124
25	柏坤桃, 柏玉升, 陈闻, 等. 江苏省高邮市猪圆环病毒病和猪繁殖与呼吸综合征流行病学调查[J]. 畜牧与兽医, 2008, 40 (12): 75- 77.
	BO K T , BO Y S , CHEN W , et al. Epidemiological survey on porcine circovirus disease and porcine reproductive and respiratory syndrome in Gaoyou City, Jiangsu province[J]. Animal Husbandry & Veterinary Medicine, 2008, 40 (12): 75- 77.
26	杜庆川. 江苏省规模猪场主要病毒性疾病的流行病学调查[D]. 呼和浩特: 内蒙古农业大学, 2023.
	DU Q C. Epidemiological investigation of major viral diseases in large-scale pig farms in Jiangsu province[D]. Hohhot: Inner Mongolia Agricultural University, 2023. (in Chinese)
27	顾秋成. 安徽地区猪繁殖与呼吸综合征血清学和分子流行病学调查[D]. 合肥: 安徽农业大学, 2016.
	GU Q C. Serological and molecular epidemiology of porcine reproductive and respiratory syndrome in Anhui[D]. Hefei: Anhui Agricultural University, 2016.
28	顾小根, 俞国乔, 陆国林. 浙江省养猪场母猪繁殖障碍症主要病因调查[J]. 中国预防兽医学报, 2002, 24 (1): 64- 65.
	GU X G , YU G Q , LU G L . Investigation on the main etiological factors of reproductive disorders in sows in pig farms in Zhejiang province[J]. Chinese Journal of Preventive Veterinary Medicine, 2002, 24 (1): 64- 65.
29	金一春, 许柯, 朱骏, 等. 2016~2020年上海市松江区猪蓝耳病的监测与分析[J]. 四川畜牧兽医, 2021, 48 (10): 25- 27.
	JIN Y C , XU K , ZHU J , et al. Surveillance and analysis of porcine blue ear disease in Songjiang District, Shanghai from 2016 to 2020[J]. Sichuan Animal & Veterinary Sciences, 2021, 48 (10): 25- 27.
30	鞠厚斌, 曹火仁, 张维谊, 等. 近年来上海地区病死猪主要病毒病感染状况调查[J]. 中国动物传染病学报, 2010, 18 (1): 40- 44.
	JU H B , CAO H R , ZHANG W Y , et al. Survey on the status of major viral disease infections in sick and dead pigs in Shanghai in recent years[J]. Chinese Journal of Animal Infectious Diseases, 2010, 18 (1): 40- 44.
31	李凌光. 2019-2022年长三角区域猪繁殖与呼吸综合征病毒的重组特征及致病性分析[D]. 北京: 中国农业科学院, 2022.
	LI L G. The recombination characteristics and pathogenicity analysis of Porcine reproductive and respiratory syndrome virus in Yangtze River Delta between2019-2022[D]. Beijing: Chinese Academy of Agricultural Sciences, 2022. (in Chinese)
32	李玉峰, 王先炜, 姜平. 上海地区出现猪繁殖与呼吸综合征和2型猪圆环病毒混合感染[J]. 中国兽医学报, 2003 (5): 442- 444.
	LI Y F , WANG X W , JIANG P . Occurrence of porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus in Shanghai Area[J]. Chinese Journal of Veterinary Science, 2003 (5): 442- 444.
33	刘爱军, 周彩琴, 孙冰冰, 等. 浙江省高致病性猪蓝耳病和猪瘟强制免疫需求调查[J]. 浙江畜牧兽医, 2019, 44 (4): 16- 18.
	LIU A J , ZHOU C Q , SUN B B , et al. The compulsory immunization requirements of PRRS and swine fever in province Zhejiang[J]. Zhejiang Journal Animal Science and Veterinary Medicine, 2019, 44 (4): 16- 18.
34	刘茂军, 甘源, 唐红慧, 等. 江苏部分地区猪高热病中猪瘟病毒和猪蓝耳病病毒的检测[J]. 金陵科技学院学报, 2009, 25 (4): 84- 88.
	LIU M J , GAN Y , TANG H H , et al. Detection of classical swine fever virus and porcine reproductive and respiratory syndrome virus in cases of high fever swine in Jiangsu[J]. Journal of Jinling Institute of Technology, 2009, 25 (4): 84- 88.
35	刘瑞琳, 黄世静, 于家荣, 等. 猪繁殖与呼吸综合征病毒SH1/2022株的分离鉴定及基因组特征分析[J/OL]. 中国动物传染病学报, 2023. (2023-06-28)[2025-09-02]. https://link.cnki.net/doi/10.19958/j.cnki.cn31-2031/s.20230628.002.
	LIU R L, HUANG S J, YU J R, et al. Isolation and genomic characterization of sh1/2022 strain of porcine reproductive and respiratory syndrome virus[J/OL]. Chinese Journal of Animal Infectious Diseases, 2023. (2023-06-28)[2025-09-02]. https://link.cnki.net/doi/10.19958/j.cnki.cn31-2031/s.20230628.002. (in Chinese)
36	刘晓东, 何海健, 姜正前, 等. 浙江部分地区猪场猪繁殖与呼吸综合征与副猪嗜血杆菌病流行现状[J]. 中国兽医杂志, 2014, 50 (5): 53- 54.
	LIU X D , HE H J , JIANG Z Q , et al. Prevalence of porcine reproductive and respiratory syndrome and haemophiles parvovirus in pig farms in some areas of Zhejiang province[J]. Chinese Journal of Veterinary Medicine, 2014, 50 (5): 53- 54.
37	钱琨, 顾丙泉, 王明珍, 等. 2007~2010年江苏地区猪繁殖与呼吸综合征病毒RT-PCR检测及部分Nsp2基因序列分析[J]. 中国动物传染病学报, 2011, 19 (4): 1- 6.
	QIAN K , GU B Q , WANG M Z , et al. RT-PCR detection and partial nsp2 gene analysis of porcine reproductive and respiratory syndrome virus in Jiangsu province from 2007 to 2010[J]. Chinese Journal of Animal Infectious Diseases, 2011, 19 (4): 1- 6.
38	沈艳, 何长生, 占松鹤, 等. 2016-2020年安徽省高致病性猪繁殖与呼吸综合征定点监测[J]. 中国动物检疫, 2021, 38 (6): 21- 25.
	SHEN Y , HE Z S , ZHAN S H , et al. Fixed-point surveillance for HP-PRRS in Anhui Province from 2016 to 2020[J]. China Animal Health Inspection, 2021, 38 (6): 21- 25.
39	童武, 周艳君, 肖少波, 等. 2010年国内部分省份猪场常见病毒性疾病的病原学调查[J]. 中国动物传染病学报, 2011, 19 (5): 1- 7.
	TONG W , ZHOU Y J , XIAO S B , et al. Epidemiological analysis of swine viral diseases in some of provinces in 2010[J]. Chinese Journal of Animal Infectious Diseases, 2011, 19 (5): 1- 7.
40	王建, 张维谊, 鞠厚斌, 等. 2004年~2012年上海地区猪繁殖与呼吸综合征流行情况调查[J]. 中国预防兽医学报, 2015, 37 (3): 190- 193.
	WANG J , ZHANG W Y , JU H B , et al. Investigation on the prevalence of porcine reproductive and respiratory syndrome in Shanghai area from 2004 to 2012[J]. Chinese Journal of Preventive Veterinary Medicine, 2015, 37 (3): 190- 193.
41	王倩, 何长生, 周迎春, 等. 2015-2018年安徽省定点猪场主要疫病病原和血清抗体监测[J]. 中国动物检疫, 2019, 36 (3): 29- 34.
	WANG Q , HE Z S , ZHOU Y C , et al. Surveillance for pathogens and serum antibodies against major diseases in fixed-point swine farms in Anhui province from 2015 to 2018[J]. China Animal Health Inspection, 2019, 36 (3): 29- 34.
42	王强, 王荣祥, 庞训胜, 等. 皖北地区猪繁殖和呼吸综合征病毒的调查[J]. 安徽农业科学, 2005, 33 (7): 1239.
	WANG Q , WANG R X , PANG X S , et al. A survey of porcine reproductive and respiratory syndrome virus in northern Anhui province, China[J]. Journal of Anhui Agricultural Sciences, 2005, 33 (7): 1239.
43	吴瑕, 劳梦琴, 谭祥梅, 等. 2017-2020年我国部分地区PRRSV流行毒株变异情况分析[J]. 中国动物传染病学报, 2021, 29 (5): 64- 74.
	WU X , LAO M Q , TAN X M , et al. Analysis on variation of PRRSV epidemic strains in some areas of China[J]. Chinese Journal of Animal Infectious Diseases, 2021, 29 (5): 64- 74.
44	徐丽华, 袁秀芳, 李军星, 等. 浙江省猪繁殖与呼吸综合征病毒ORF5基因的遗传变异分析[J]. 中国兽医学报, 2013, 33 (2): 187- 194.
	XU L H , YUAN X F , LI J X , et al. Genetic analysis of porcine reproductive and respiratory syndrome virus ORF5 gene from Zhejiang province[J]. Chinese Journal of Veterinary Science, 2013, 33 (2): 187- 194.
45	叶俊平, 盛瑜, 朱丽娜, 等. 江苏省猪高热病病例主要继发感染病毒的多重PCR检测[J]. 中国兽医科学, 2007 (12): 1029- 1034.
	YE J P , SHENG Y , ZHU L N , et al. Multiplex PCR detection of main subsequent viruses in cases of high swine fever in Jiangsu province from 2005 to 2006[J]. Chinese Veterinary Science, 2007 (12): 1029- 1034.
46	叶向阳, 张婉华, 祈贤, 等. 上海地区猪繁殖-呼吸综合征血清学检测及病毒分离与鉴定[J]. 上海畜牧兽医通讯, 1999 (6): 7- 8.
	YE X Y , ZHANG W H , QI X , et al. Serological detection, virus isolation and identification of porcine reproductive-respiratory syndrome in the Shanghai area[J]. Shanghai Journal of Animal Husbandry and Veterinary Medicine, 1999 (6): 7- 8.
47	张维谊, 鞠厚斌, 周锦萍, 等. 上海及周边地区散养猪主要病毒病的流行状况调查[J]. 动物医学进展, 2008 (7): 36- 38.
	ZHANG W Y , JU H B , ZHOU J P , et al. Epidemic investigation on main swine virus disease in small-scale pig farms in Shanghai and peripheral areas[J]. Progress in Veterinary Medicine, 2008 (7): 36- 38.
48	赵永旺, 王子轼, 朱达文, 等. 猪繁殖与呼吸综合征病毒和猪瘟病毒混合感染的调查[J]. 江苏农业科学, 2010 (3): 292- 293.
	ZHAO Y W , WANG Z S , ZHU D W , et al. Investigation of mixed infections of porcine reproductive and respiratory syndrome virus and swine fever virus[J]. Jiangsu Agricultural Sciences, 2010 (3): 292- 293.
49	祝闰琦, 崔雪娇, 何长生, 等. 安徽地区猪繁殖与呼吸综合征病毒临床感染与流行毒株的监测与分析[J]. 畜牧与兽医, 2020, 52 (6): 128- 132.
	ZHU R Q , XUE J C , HE C S , et al. Epidemiological investigation of PRRSV in Anhui Province[J]. Animal Husbandry & Veterinary Medicine, 2020, 52 (6): 128- 132.
50	李文忠, 荣新利, 刘野, 等. 天津地区猪繁殖与呼吸综合征病毒流行毒株ORF5基因的克隆及遗传变异分析[J]. 中国动物传染病学报, 2025, 33 (2): 156- 164.
	LI W Z , RONG X L , LIU Y , et al. Cloning and genetic variation analysis of ORF5 gene of porcine reproductive and respiratory syndrome epidemic strains in Tianjin[J]. Chinese Journal of Animal Infectious Diseases, 2025, 33 (2): 156- 164.
51	袁志乔. 河南地区猪繁殖与呼吸综合征病毒遗传变异分析[D]. 南阳: 南阳师范学院, 2022.
	YUAN Z Q. Genetic variation analysis of porcine Reproductive and respiratory syndrome virus in Henan province[D]. Nanyang: Nanyang Normal University, 2022. (in Chinese)
52	王志远, 刘博奇, 许志颖, 等. 2021—2022年我国部分地区猪繁殖与呼吸综合征病毒ORF5基因变异分析[J]. 畜牧兽医学报, 2023, 54 (9): 3812- 3823. doi: 10.11843/j.issn.0366-6964.2023.09.021
	WANG A Y , LIU B Q , XU Z Y , et al. Analysis of orf5 gene variation of porcine reproductive and respiratory syndrome virus in local regions of China from 2021 to 2022[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54 (9): 3812- 3823. doi: 10.11843/j.issn.0366-6964.2023.09.021
53	钟佳欢, 李德龙, 张诗琦, 等. 猪繁殖与呼吸综合征研究进展[J]. 养猪, 2024 (4): 43- 46.
	ZHONG J H , LI D L , ZHANG S Q , et al. Advances in research on porcine reproductive and respiratory syndrome[J]. Swine Production, 2024 (4): 43- 46.
54	张力超. 高致病性猪蓝耳病RT-PCR检测[J]. 吉林畜牧兽医, 2024, 45 (2): 34- 36.
	ZHANG L C . Detection of highly pathogenic porcine blue ear disease by RT-PCR[J]. Jilin Animal Husbandry and Veterinary Medicine, 2024, 45 (2): 34- 36.
55	刘英杰, 齐六卫, 董彦龙, 等. 吉林某规模化猪场的猪蓝耳病抗原抗体分析及防控建议[J]. 猪业科学, 2022, 39 (11): 77- 79.
	LIU Y J , QI L W , DONG Y L , et al. Analysis of antigenic antibodies to porcine blue ear disease in a large-scale pig farm in Jilin, China, and recommendations for prevention and control of the disease[J]. Swine Industry Science, 2022, 39 (11): 77- 79.
56	张慧勤. 猪蓝耳病在我国的防控现状[J]. 畜牧兽医科技信息, 2020 (12): 162.
	ZHANG H Q . The current situation of pig blue ear disease prevention and control in China[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2020 (12): 162.
57	高延娜, 宋丽霞, 包小萌, 等. 高致病性猪蓝耳病研究进展[J]. 山东畜牧兽医, 2016, 37 (12): 85- 86.
	GAO Y N , SONG L X , BAO X M , et al. Research progress on highly pathogenic porcine reproductive and respiratory syndrome[J]. Shandong Journal of Animal Science and Veterinary Medicine, 2016, 37 (12): 85- 86.

序号 No.	文献质量评估工具 Tools for assessing the quality of literature
1	样本框架是否适合解决目标猪群的问题? Was the sample frame appropriate to address the target pig?
2	研究是否采用了合适的抽样方法? Were study participants sampled in an appropriate way?
3	样本量是否足够? Was the sample size adequate?
4	数据分析是否覆盖了目标样本? Was the data analysis conducted with sufficient coverage of the identified sample?
5	是否采用了有效的方法计算发病率? Were valid methods used for the identification of the condition?
6	是否以标准、可靠的方式评估所有样本? Was the condition measured in a standard, reliable way for all participants?
7	是否进行了适当的统计分析? Was there appropriate statistical analysis?
8	研究对象和研究环境是否详细描述? Were the study subjects and the setting described in detail?

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中国长三角地区猪繁殖与呼吸综合征病毒流行性Meta分析

Meta-analysis of Porcine Reproductive and Respiratory Syndrome Virus Prevalence in China's Yangtze River Delta Region

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