聚苯乙烯纳米塑料对公鸭睾丸的损伤作用

doi:10.11843/j.issn.0366-6964.2025.02.040

摘要/Abstract

摘要：

旨在揭示聚苯乙烯纳米塑料(PS-NPs)的雄性生殖毒性，本试验以雄性绍兴鸭为研究对象，探究了PS-NPs对鸭睾丸组织的影响。试验将32只绍兴公鸭随机分为空白Control组、L-NPs组、M-NPs组和H-NPs组共4组(每组8只)，分别饲喂饲料浓度为0、1、10、100 mg·kg^-1的PS-NPs。45 d称重后致死，采集鸭的睾丸，计算睾丸系数，HE染色观察各组睾丸组织病理变化；试剂盒检测睾丸组织中丙二醛(MDA)含量以及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物岐化酶(SOD)和过氧化氢酶(CAT)活性；Western blot检测氧化损伤相关蛋白Nrf2、NQO1和HO-1的表达水平；实时荧光定量PCR检测炎症相关基因TNF-α和IL-6的mRNA转录水平。结果显示，与Control组相比，随着PS-NPs处理浓度升高，H-NPs组睾丸系数显著降低(P＜0.05)；病理学结果显示，曲精小管间的间隙不断变大，间质细胞数量逐渐减少，曲精小管管壁结构模糊；M-NPs组和H-NPs组睾丸组织MDA含量显著(P＜0.05)和极显著(P＜0.01)升高，GSH、SOD和CAT活性显著(P＜0.05)和极显著(P＜0.01)下降；Western blot结果显示，与Control组相比，M-NPs组和H-NPs组Nrf2、NQO1、HO-1蛋白表达水平呈显著(P＜0.05)和极显著(P＜0.01)下降；实时荧光定量PCR结果显示，与Control组相比，M-NPs组和H-NPs组TNF-α、IL-6基因mRNA转录水平显著(P＜0.05)和极显著(P＜0.01)升高。结果表明，PS-NPs能引起绍兴鸭睾丸组织形态结构改变、炎症和氧化应激，并且其损伤程度与PS-NPs暴露剂量呈正相关；PS-NPs引起的炎症反应与Nrf2/HO-1信号通路密切相关。

关键词: 聚苯乙烯纳米塑料, 鸭, 睾丸, 氧化应激, 炎症

Abstract:

In order to reveal the male reproductive toxicity of polystyrene nanoplastics (PS-NPs), this study investigated the effects of PS-NPs on duck testicular tissue using male Shaoxing ducks. In this study, 32 Shaoxing ducks were randomly divided into 4 groups(8 ducks in each group), namely, blank Control group, L-NPs group, M-NPs group, and H-NPs group, and were fed with PS-NPs at dietary concentrations of 0, 1, 10 and 100 mg·kg^-1. After 45 days, the body weight of the ducks were weighed, the testes of the ducks were collected, testis coefficients were calculated, the pathological changes of testis tissues of each group were observed by HE staining. The testicular tissues were tested for malondialdehyde (MDA) content and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) by kit. The expression levels of oxidative damage-related proteins Nrf2, NQO1 and HO-1 were detected by Western blot. The mRNA levels of inflammation-related genes TNF-α and IL-6 were detected by real-time fluorescent quantitative PCR. The results showed that the testicular coefficient was significantly reduced in the H-NPs group with the increase of PS-NPs treatment concentration compared with the control group (P<0.05). The pathological results showed that the interstices between the convoluted seminiferous tubules were continuously enlarged, the number of interstitial cells was gradually reduced, and the structure of convoluted tubule wall was blurred. The testicular tissues of the M-NPs group and H-NPs group had a significant (P<0.05) and highly significant (P<0.01) increase in the content of MDA. The activity of GSH, SOD and CAT decreased significantly (P<0.05) and very significantly (P<0.01). Western blot results showed that the protein expression levels of Nrf2, NQO1 and HO-1 present a trend of significant (P<0.05) or highly significant decrease (P<0.01) in the M-NPs group and H-NPs group. The real-time fluorescence quantitative PCR results showed that the mRNA transcript levels of TNF-α and IL-6 genes were significantly (P<0.05) and highly significant increased (P<0.01). The results showed that PS-NPs could cause morphological and structural changes, inflammation and oxidative stress in the testicular tissues of Shaoxing ducks. The degree of damage was positively correlated with the dose of PS-NPs exposure. The inflammatory response caused by PS-NPs may be closely related to the Nrf2/HO-1 signaling pathway.

Key words: polystyrene nanoplastics, duck, testicle, oxidative stress, inflammation

中图分类号:

顾雅怡, 夏苏干, 刘鹏利, 邹辉, 顾建红, 袁燕, 刘学忠, 刘宗平, 卞建春. 聚苯乙烯纳米塑料对公鸭睾丸的损伤作用[J]. 畜牧兽医学报, 2025, 56(2): 925-933.

GU Yayi, XIA Sugan, LIU Pengli, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Damaging Effect of Polystyrene Nanoplastics on the Testicles of Male Ducks[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(2): 925-933.

图/表 6

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基因 Genes	引物序列(5′→3′) Primer sequences
TNF-α	ACAGGACAGCCTATGCCAAC
	ACAHHAAHHHCAACACATCT
IL-6	TGGCTTCGACGAGGAGAAA
	AGCTGAATCTGGGATGACCAC
β-Actin	ATGTCGCCCTGGATTTCG
	CACAGGACTCCATACCCAAGAAT

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