赖氨酸乙酰化修饰在鸡精液冷冻中的保护作用及机制研究进展

doi:10.11843/j.issn.0366-6964.2025.07.003

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (7): 3071-3079.doi: 10.11843/j.issn.0366-6964.2025.07.003

赖氨酸乙酰化修饰在鸡精液冷冻中的保护作用及机制研究进展

宗云鹤¹(), 杨宇泽²(), 孙研研¹, 陈继兰¹, 李云雷¹^,*()

1. 中国农业科学院北京畜牧兽医研究所，畜禽生物育种全国重点实验室，北京 100193
2. 北京市畜牧总站，北京 100107

收稿日期:2024-12-09 出版日期:2025-07-23 发布日期:2025-07-25
通讯作者: 李云雷 E-mail:zongyunhe2022@163.com;yyz84929056@126.com;liyunlei@caas.cn
作者简介:宗云鹤(1996-)，女，辽宁抚顺人，博士生，主要从事动物遗传育种与繁殖研究，E-mail: zongyunhe2022@163.com
杨宇泽(1982-)，男，山西平鲁人，高级畜牧师，硕士，主要从事畜禽遗传资源保护与利用研究，E-mail: yyz84929056@126.com
第一联系人：
宗云鹤和杨宇泽为同等贡献作者
基金资助:
国家自然科学基金(32302726)；国家重点研发计划项目(2021YFD1200305)；北京市特色畜禽遗传资源保护项目(202203310002)

Research Advances in the Investigation of the Protective Role of Lysine Acetylation in Chicken Semen Cryopreservation and Its Mechanism

ZONG Yunhe¹(), YANG Yuze²(), SUN Yanyan¹, CHEN Jilan¹, LI Yunlei¹^,*()

1. State Key Laboratory of Animal Biotechnology Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2. Beijing General Station of Animal Husbandry Service, Beijing 100107, China

Received:2024-12-09 Online:2025-07-23 Published:2025-07-25
Contact: LI Yunlei E-mail:zongyunhe2022@163.com;yyz84929056@126.com;liyunlei@caas.cn

摘要/Abstract

摘要：

我国地方鸡种140个，遗传变异丰富，开展鸡遗传资源的保护、开发与利用，对于促进我国鸡种业自主发展具有重要意义。精液的超低温冷冻保存是实现畜禽遗传资源长期保存与高效利用的最实用的技术手段之一。家畜尤其牛的精液冷冻技术较为成熟，但鸡精子头小尾长、抗冻性差，冷冻-复融精子受精率低、受精持续能力差，制约着精液冷冻技术在鸡保种和育种中的应用。蛋白质翻译后修饰是细胞响应内外界环境刺激的重要调控途径。在成熟精子中，由于转录和翻译活动基本停滞，蛋白质赖氨酸乙酰化修饰可能在鸡精液冷冻保护过程中发挥作用，这些修饰可参与维持精子结构完整性、线粒体功能稳定性以及DNA结构完整性，然而其具体机制尚不清楚。本综述以期为开展赖氨酸乙酰化修饰在鸡精液冷冻保存中的作用相关研究提供参考，以建立鸡精液冷冻保存新方法，为鸡遗传资源的高效保存提供理论与技术支撑。

关键词: 鸡, 冷冻精液, 精子, 赖氨酸乙酰化修饰, 种质资源

Abstract:

China has a rich diversity of chicken breeds with 140 native breeds, which harbor numerous excellent genetic traits. The conservation, breeding, and utilization of chicken genetic resources are of significant importance for promoting the independent development of national chicken breeding industry. The ultra-low temperature cryopreservation of semen is one of the most practical techniques for the long-term preservation and efficient utilization of livestock and poultry genetic resources. The semen cryopreservation technology for livestock, particularly for cattle, is relatively well-developed, but the technology was limited in chicken due to the long tails and small heads of spermatozoa, which result in poor freeze resistance. The poor fertility and sustained fertilization ability of chicken frozen sperm limited the application of semen cryopreservation technique in conservation and breeding. Post-translational modification of proteins was an important regulatory pathway through which cells respond to internal and external environmental stimuli. In mature sperm, both transcription and translation activities were largely halted, lysine acetylation modification of proteins might play crucial roles during the cryopreservation of chicken semen. These modifications could be essential for maintaining sperm structural integrity, mitochondrial function stability, and DNA integrity. However, the specific mechanisms underlying these effects remain unclear. This review aimed to provide a reference for investigating the role of lysine acetylation in the cryopreservation of chicken semen and establishing novel cryopreservation methods, and to offer theoretical insights and technical support for the efficient conservation of poultry genetic resources.

Key words: chicken, semen cryopreservation, sperm, lysine acetylation modification, germplasm resource

中图分类号:

S831.3

宗云鹤, 杨宇泽, 孙研研, 陈继兰, 李云雷. 赖氨酸乙酰化修饰在鸡精液冷冻中的保护作用及机制研究进展[J]. 畜牧兽医学报, 2025, 56(7): 3071-3079.

ZONG Yunhe, YANG Yuze, SUN Yanyan, CHEN Jilan, LI Yunlei. Research Advances in the Investigation of the Protective Role of Lysine Acetylation in Chicken Semen Cryopreservation and Its Mechanism[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3071-3079.

图/表 4

图 1

表 1

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图 2

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家族 Family	酶(别名) Enzyme (alias)	特异性抑制剂 Specific inhibitor	睾丸 Testis	精子 Sperm	精浆 Seminal plasma
GNAT	HAT1 (KAT1)	CPTH6	-	√	-
	GCN5 (KAT2A)	CPTH2 (C₁₄H₁₄ClN₃S)	√	-	-
MYST	Tip60 (KAT5)	TH1834 (C₃₃H₄₀N₆O₃)	-	√	-
	MORF (KAT6B)	-	-	√	√
其他Other	TAF1(KAT4)	CeMMEC13(C₁₉H₁₆N₂O₄)	√	-	-

家族 Family	酶 Enzyme	通用抑制剂 Universal inhibitor	特异性抑制剂 Specific inhibitor	睾丸 Testis	精子 Sperm	精浆 Seminal plasma
HDAC Zn²⁺依赖型 Zn²⁺-dependent	HDAC1	TSA (C₁₇H₂₂N₂O₃)	HDAC1-IN-4 (C₂₁H₂₄BrClN₆O₂)	√	-	-
	HDAC2		HDAC2-IN-1 (C₂₂H₂₃ClN₄OS)	√	-	-
	HDAC8		HDAC8-IN-3 (C₁₈H₁₂N₄O₃S₂)	-	√	-
	HDAC7		-	√	-	-
	HDAC10		HDAC10-IN-2 (C₁₉H₂₂N₂O₂)	-	√	-
	HDAC11		FT895 (C₁₆H₁₅F₃N₄O₂)	√	-	-
SIRT NAD+依赖型 NAD⁺-dependent	SIRT2	NAM(C₆H₆N₂O)	SirReal2 (C₂₂H₂₀N₄OS₂)	√	√	-
	SIRT3		3-TYP (C₇H₆N₄)	√	√	-
	SIRT5		NRD167 (C34H46N6O6S)	√	√	-

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赖氨酸乙酰化修饰在鸡精液冷冻中的保护作用及机制研究进展

Research Advances in the Investigation of the Protective Role of Lysine Acetylation in Chicken Semen Cryopreservation and Its Mechanism

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