水獭源犬圆环病毒的全基因组序列分析

doi:10.11843/j.issn.0366-6964.2025.04.045

摘要/Abstract

摘要：

犬圆环病毒(canine circovirus, CanineCV)是一种与犬消化道疾病相关的病毒，该病毒于2012年在美国首次从犬中鉴定。随后，研究人员相继在狼、獾、北极狐、赤狐、豺中检测到了CanineCV。2016年，我国首次报道从犬中检测到CanineCV，然而，关于CanineCV在我国野生动物中的感染情况尚不清楚。采集一病死水獭的组织病料，利用PCR进行CanineCV的检测；设计CanineCV全基因组引物，对CanineCV阳性样本进行全基因组扩增、测序和基因序列分析。结果显示：通过PCR检测，发现1份水獭组织样本CanineCV阳性；通过全基因组扩增、测序、拼接，成功获得一条长度为2 063 nt的CanineCV全基因组序列；核苷酸同源性分析显示，该毒株与GenBank中其他毒株的基因序列同源性为84.6%~98.2%；遗传进化树分析发现，该毒株属于CanineCV-3型。首次在水獭中鉴定出CanineCV的感染，并成功获得了我国首条野生动物源CanineCV全基因组序列及其遗传进化信息。以上结果表明CanineCV感染水獭的潜在危害及其感染更多其他动物的可能性，从而提示人们应加强CanineCV感染不同动物的监测。

关键词: 犬圆环病毒, 水獭, 流行病学, 遗传进化分析

Abstract:

Canine circovirus (CanineCV) is a virus associated with canine gastrointestinal diseases. Since it was first identified from dogs in 2012 in the United States. Researchers have subsequently detected the presence of CanineCV in various wildlife species, including wolves (Canis lupus), badgers (Meles meles), red foxes (Vulpes vulpes), arctic foxes (Vulpes lagopus) and jackals(Lupulella mesomelas).In 2016, CanineCV was first reported in dogs in China. However, a systematic investigation of CanineCV infection in wildlife in China remains lacking. We acquired tissue specimens from an ill and deceased otter (Lutra lutra), and successfully identified the presence of CanineCV through the application of Polymerase Chain Reaction (PCR). We designed of CanineCV genome-wide primers for whole genome amplification, sequenced and analyzed the direction of genetic evolution. Result were as follows: Through PCR technology, we tested the otter tissue samples for CanineCV, and the results were positive. By whole-genome amplification, sequencing, and assembly, we successfully obtained a CanineCV whole-genome sequence with a length of 2 063 nt. Nucleotide homology analysis revealed that this strain had a gene sequence homology of 84.6%-98.2% with all other strains in GenBank. After constructing a genetic evolution tree, we found that this strain belonged to CanineCV-3. This is the first identification of CanineCV infection in an otter, and we have successfully obtained the first whole-genome sequence and genetic evolution information of CanineCV derived from wildlife in China. The above results illustrate the potential hazards of CanineCV infecting otters and their possibility of infecting more other animals, thus suggesting that we should strengthen the monitoring of CanineCV infection in different animals.

Key words: canine circovirus, Lutra lutra, epidemiology, genetic evolutionary analysis

中图分类号:

S852.659.2

匡尹之, 徐凤培, 周沛. 水獭源犬圆环病毒的全基因组序列分析[J]. 畜牧兽医学报, 2025, 56(4): 1989-1994.

KUANG Yinzhi, XU Fengpei, ZHOU Pei. Whole Genome Sequence Analysis of Canine Circovirus in an Otter (Lutra lutra)[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1989-1994.

图/表 3

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引物名称 Primers name	序列(5′→3′) Sequences	扩增长度/ bp Products length
CanineCV-f1	CAAFCTTGCGGCCGCGAATTCCGCGCGAGGGCGTTTACCTGTTCA	1 996
CanineCV-r1	CTCTAGAGTCGACTGGTACCGTGCTTCGGGCGGAGCGAGA
CanineCV-f2	CAAGCTTGCGGCCGCGAATTCTGAAAGATAAAGGCCTCTC	144
CanineCV-r2	CTCTAGAGTCGACTGGTACCTATWCAGCCTCGAAGGG

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