海南黑山羊ATG16L2基因启动子区多态性研究

doi:10.11843/j.issn.0366-6964.2024.11.017

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (11): 4980-4991.doi: 10.11843/j.issn.0366-6964.2024.11.017

海南黑山羊ATG16L2基因启动子区多态性研究

王欢¹(), 陈韬羽¹, 吴慧², 蒙勇¹, 李世元¹, 钱和洁¹, 牛世华¹, 满初日嘎¹, 陈巧玲¹, 高宏岩¹, 杜丽¹, 王凤阳¹, 陈思¹^,*()

1. 海南大学热带农林学院/海南省热带动物繁育与疫病研究重点实验室, 海口 570228
2. 新疆维吾尔自治区哈密市巴里坤哈萨克自治县畜牧兽医工作站, 哈密 839202

收稿日期:2024-05-14 出版日期:2024-11-23 发布日期:2024-11-30
通讯作者: 陈思 E-mail:772101737@qq.com;chensi.ruth@hotmail.com
作者简介:王欢(1997-), 女, 河南南阳人, 硕士生, 主要从事动物遗传育种与繁殖研究, E-mail: 772101737@qq.com
基金资助:
国家自然科学基金青年基金项目(32202631);国家现代农业产业技术体系项目(财政部和农业农村部-CARS38)

Polymorphism Analysis of the ATG16L2 Gene Promoter Region in Hainan Black Goat

Huan WANG¹(), Taoyu CHEN¹, Hui WU², Yong MENG¹, Shiyuan LI¹, Hejie QIAN¹, Shihua NIU¹, Churiga MAN¹, Qiaoling CHEN¹, Hongyan GAO¹, Li DU¹, Fengyang WANG¹, Si CHEN¹^,*()

1. Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research of Hainan Province, College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
2. Animal Husbandry and Veterinary Workstation of Barkol Kazak Autonomous County, Hami City, Xinjiang Uygur Autonomous Region, Hami 839202, China

Received:2024-05-14 Online:2024-11-23 Published:2024-11-30
Contact: Si CHEN E-mail:772101737@qq.com;chensi.ruth@hotmail.com

摘要/Abstract

摘要：

旨在研究海南黑山羊ATG16L2基因启动子区的结构特征及其遗传分布情况，为进一步探索该基因的表达调控机制及功能提供理论依据。本研究以200头海南黑山羊为研究对象，构建DNA混池，采用Sanger法测序对海南黑山羊ATG16L2基因启动子区的多态性进行初筛，应用PCR-RFLP技术对200头海南黑山羊个体进行基因型鉴定。对筛选到的SNP位点进行连锁不平衡分析，构建单倍型。利用生物信息学方法分析SNP位点对海南黑山羊ATG16L2基因表达的影响。在海南黑山羊ATG16L2基因启动子区共检测到3个SNPs位点，分别为SNP1(g.30667970T＞C)、SNP2(g.30668540T＞C)和SNP3(g.30668664C＞T)，且彼此连锁。SNP1和SNP2位点均表现为中度多态性，SNP3位点表现为低度多态性，且符合Hardy-Weinberg平衡(P＞0.05)。单倍型分析结果显示，H1、H2、H3和H4单倍型频率分别为0.321、0.304、0.271和0.097，且H1(CGC)为优势单倍型。生物信息学分析显示，山羊ATG16L2基因共预测到3个启动子和4个CpG岛区域；存在2个重复元件LINE2(—1 989~—1 826 bp、—562~—426 bp)、hAT-Charlie (—1 804~—1 511 bp)以及5个CCAAT-Box、13个CAAT-Box、10个CGCG-Box、11个GATA-Box和2个TATA-Box。综合多种在线软件预测发现，上述SNPs可能通过影响ATG16L2基因的启动子区的顺式作用元件，从而影响海南黑山羊ATG16L2基因的转录表达。本研究在海南黑山羊ATG16L2基因启动子序列中发现3个SNPs位点，其中SNP1和SNP2表现为中度多态性，SNP3表现为低度多态性，并预测这些SNPs可能影响转录因子结合，从而调控基因表达，为进一步探究ATG16L2基因功能及其调控机制提供了理论依据。

关键词: 海南黑山羊, ATG16L2基因, 启动子, 多态性, 生物信息学

Abstract:

The study aimed to investigate the structural characteristics and genetic distribution of the ATG16L2 gene promoter region in Hainan Black goat, providing a theoretical basis for further exploring the expression regulation mechanism and function of this gene. This study focused on 200 Hainan Black goats, constructing a DNA pooling strategy. The polymorphism of the ATG16L2 gene promoter region in Hainan Black goats was initially screened using Sanger sequencing. PCR-RFLP technology was applied for genotyping the 200 Hainan Black goats. Linkage disequilibrium analysis was performed on the identified SNP loci to construct haplotypes. Bioinformatics methods were utilized to analyze the impact of SNP loci on the expression of the ATG16L2 gene in Hainan Black goats. The promoter region of the ATG16L2 gene in Hainan Black goats contained 3 linked single nucleotide polymorphisms (SNPs): SNP1 (g.30667970T > C), SNP2 (g.30668540T > C), and SNP3 (g.30668664C > T). Both SNP1 and SNP2 exhibited moderate levels of polymorphism, whereas SNP3 showed low levels. Notably, all three SNPs conformed to the principles of Hardy-Weinberg equilibrium(P > 0.05). The haplotype analysis revealed that the frequencies of haplotype H1, H2, H3, and H4 were 0.321, 0.304, 0.271, and 0.097, respectively, with H1 (CGC) being the predominant haplotype. The bioinformatics analysis results showed that there were 3 promoters, 4 CpG island regions, 2 repetitive elements LINE2 (—1 989-—1 826 bp, —562-—426 bp), hAT-Charlie (—1 804-—1 511 bp), and 5 CCAAT-Box, 13 CAAT-Box, 10 CGCG-Box, 11 GATA-Box and 2 TATA-Box of the ATG16L2 gene in Hainan Black goat. The integrated prediction from various online tools suggests that the above SNPs may affect the expression regulation of the ATG16L2 gene in Hainan Black goats by influencing the changes in transcription factors in the gene's promoter region. In this study, 3 SNP loci were identified in the promoter sequence of the ATG16L2 gene in Hainan Black goats. Among these, SNP1 and SNP2 exhibited moderate polymorphism, while SNP3 showed low polymorphism. It is predicted that these SNPs may influence transcription factor binding, thereby regulating gene expression. This provides a theoretical basis for further exploration of the function and regulatory mechanisms of the ATG16L2 gene.

Key words: Hainan Black goat, ATG16L2 gene, promoter, polymorphism, bioinformatics

中图分类号:

S827.2

王欢, 陈韬羽, 吴慧, 蒙勇, 李世元, 钱和洁, 牛世华, 满初日嘎, 陈巧玲, 高宏岩, 杜丽, 王凤阳, 陈思. 海南黑山羊ATG16L2基因启动子区多态性研究[J]. 畜牧兽医学报, 2024, 55(11): 4980-4991.

Huan WANG, Taoyu CHEN, Hui WU, Yong MENG, Shiyuan LI, Hejie QIAN, Shihua NIU, Churiga MAN, Qiaoling CHEN, Hongyan GAO, Li DU, Fengyang WANG, Si CHEN. Polymorphism Analysis of the ATG16L2 Gene Promoter Region in Hainan Black Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 4980-4991.

图/表 13

表 1

表 2

图 1

表 3

图 2

图 3

图 4

图 5

表 4

图 6

表 5

表 6

图 7

参考文献 39

1	RIZZOLLO F , MORE S , VANGHELUWE P , et al. The lysosome as a master regulator of iron metabolism[J]. Trends Biochem Sci, 2021, 46 (12): 960- 975. doi: 10.1016/j.tibs.2021.07.003
2	ALBANO G D , MONTALBANO A M , GAGLIARDO R , et al. Autophagy/mitophagy in airway diseases: impact of oxidative stress on epithelial cells[J]. Biomolecules, 2023, 13 (8): 1217. doi: 10.3390/biom13081217
3	ALULA K M , THEISS A L . Autophagy in Crohn's disease: converging on dysfunctional innate immunity[J]. Cells, 2023, 12 (13): 1779. doi: 10.3390/cells12131779
4	CHEN L , YANG L M , LI Y Y , et al. Autophagy and inflammation: regulatory roles in viral infections[J]. Biomolecules, 2023, 13 (10): 1454. doi: 10.3390/biom13101454
5	HU J T , ZHAO W , NIU L L , et al. Gene organization and characterization of the complete mitochondrial genome of Hainan black goat (Capra hircus)[J]. Mitochondrial DNA A DNA Mapp Seq Anal, 2016, 27 (3): 1656- 1657.
6	WANG D F , ZHOU L L , ZHOU H L , et al. Effects of nutrition level of concentrate-based diets on growth performance and carcass characteristics of Hainan black goats[J]. Trop Anim Health Prod, 2014, 46 (5): 783- 788. doi: 10.1007/s11250-014-0565-x
7	SHI L G , ZHANG Y , WU L L , et al. Moderate coconut oil supplement ameliorates growth performance and ruminal fermentation in Hainan black goat kids[J]. Front Vet Sci, 2020, 7, 622259. doi: 10.3389/fvets.2020.622259
8	WANG D Y , YUAN T L , LIU J M , et al. ATG16L2 inhibits NLRP3 inflammasome activation through promoting ATG5-12-16L1 complex assembly and autophagy[J]. Eur J Immunol, 2022, 52 (8): 1321- 1334. doi: 10.1002/eji.202149764
9	MO Y J , ZHANG W , WEN Q W , et al. Corrigendum to "Genetic association analysis of ATG16L1 rs2241880, rs6758317 and ATG16L2 rs11235604 polymorphisms with rheumatoid arthritis in a Chinese population"[Int. Immunopharmacol. 93 (2021) 107378][J]. Int Immunopharmacol, 2022, 104, 108511. doi: 10.1016/j.intimp.2021.108511
10	LUU L D W , POPPLE G , TSANG S P W , et al. Genetic variants involved in innate immunity modulate the risk of inflammatory bowel diseases in an understudied Malaysian population[J]. J Gastroenterol Hepatol, 2022, 37 (2): 342- 351. doi: 10.1111/jgh.15752
11	MA T , WU S , YAN W , et al. A functional variant of ATG16L2 is associated with Crohn's disease in the Chinese population[J]. Colorectal Dis, 2016, 18 (11): O420- O426.
12	MOLINEROS J E , YANG W L , ZHOU X J , et al. Confirmation of five novel susceptibility loci for systemic lupus erythematosus (SLE) and integrated network analysis of 82 SLE susceptibility loci[J]. Hum Mol Genet, 2017, 26 (6): 1205- 1216.
13	ZHONG C H , WANG Y Y , LIU C P , et al. A novel single-nucleotide polymorphism in WNT4 promoter affects its transcription and response to FSH in chicken follicles[J]. Genes (Basel), 2022, 13 (10): 1774. doi: 10.3390/genes13101774
14	LI K Y , LIU Y F , HE X Y , et al. A novel SNP in the promoter region of IGF1 associated with Yunshang black goat kidding number via promoting transcription activity by SP1[J]. Front Cell Dev Biol, 2022, 10, 873095. doi: 10.3389/fcell.2022.873095
15	ZHANG P , FU Y , ZHANG R , et al. Association of KCTD15 gene with fat deposition in pigs[J]. J Anim Physiol Anim Nutr (Berl), 2022, 106 (3): 537- 544. doi: 10.1111/jpn.13587
16	WANG P , LI W T , LIU Z Y , et al. Analysis of the association of two SNPs in the promoter regions of the PPP2R5C and SLC39A5 genes with litter size in Yunshang black goats[J]. Animals (Basel), 2022, 12 (20): 2801.
17	MAŃKOWSKA A , BRYM P , SOBIECH P , et al. Promoter polymorphisms in STK35 and IFT27 genes and their associations with boar sperm freezability[J]. Theriogenology, 2022, 189, 199- 208. doi: 10.1016/j.theriogenology.2022.06.023
18	ROY J , ANAND K , MOHAPATRA S , et al. Single nucleotide polymorphisms in piRNA-pathway genes: an insight into genetic determinants of human diseases[J]. Mol Genet Genomics, 2020, 295 (1): 1- 12. doi: 10.1007/s00438-019-01612-5
19	WORKU D , GOWANE G , VERMA A . Genetic variation in promoter region of the bovine LAP3 gene associated with estimated breeding values of milk production traits and clinical mastitis in dairy cattle[J]. PLoS One, 2023, 18 (5): e0277156. doi: 10.1371/journal.pone.0277156
20	ANJUM K H , NADEEM A , JAVED M , et al. Genomic and computational analysis of novel SNPs in TNP1 gene promoter region of Bos indicus breeding bulls[J]. Genet Res (Camb), 2022, 2022, 9452234.
21	YUAN Z H , GE L , SU P W , et al. NCAPG regulates myogenesis in sheep, and SNPs located in its putative promoter region are associated with growth and development traits[J]. Animals (Basel), 2023, 13 (20): 3173.
22	WORKU D , GOWANE G , ALEX R , et al. Inputs for optimizing selection platform for milk production traits of dairy Sahiwal cattle[J]. PLoS One, 2022, 17 (5): e0267800. doi: 10.1371/journal.pone.0267800
23	WORKU D , GOWANE G R , MUKHERJEE A , et al. Associations between polymorphisms of LAP3 and SIRT1 genes with clinical mastitis and milk production traits in Sahiwal and Karan Fries dairy cattle[J]. Vet Med Sci, 2022, 8 (6): 2593- 2604. doi: 10.1002/vms3.924
24	HAN Y C , TAN T , LI Z X , et al. Identification of selection signatures and loci associated with important economic traits in Yunan black and Huainan pigs[J]. Genes (Basel), 2023, 14 (3): 655. doi: 10.3390/genes14030655
25	YAO D W , GUO D C , ZHANG Y K , et al. Identification of mutations in porcine STAT5A that contributes to the transcription of CISH[J]. Front Vet Sci, 2023, 9, 1090833. doi: 10.3389/fvets.2022.1090833
26	CHEN S , CHAI M L , TIAN C , et al. Genetic variants of fatty acid elongase 6 in Chinese Holstein cow[J]. Gene, 2018, 670, 123- 129. doi: 10.1016/j.gene.2018.05.073
27	KHOR B , CONWAY K L , OMAR A S , et al. Distinct tissue-specific roles for the disease-associated autophagy genes ATG16L2 and ATG16L1[J]. J Immunol, 2019, 203 (7): 1820- 1829. doi: 10.4049/jimmunol.1800419
28	ZHU Z J , HE M N , ZHANG T , et al. LSD1 promotes the FSH responsive follicle formation by regulating autophagy and repressing Wt1 in the granulosa cells[J]. Sci Bull (Beijing), 2024, 69 (8): 1122- 1136. doi: 10.1016/j.scib.2024.01.015
29	LUO Y B , XU Q , XUE M M , et al. Novel haplotype in the HHEX gene promoter associated with body length in pigs[J]. Genes (Basel), 2023, 14 (2): 511. doi: 10.3390/genes14020511
30	HATTORI N , LIU Y Y , USHIJIMA T . DNA methylation analysis[J]. Methods Mol Biol, 2023, 2691, 165- 183.
31	LENTJES M H , NIESSEN H E , AKIYAMA Y , et al. The emerging role of GATA transcription factors in development and disease[J]. Expert Rev Mol Med, 2016, 18, e3. doi: 10.1017/erm.2016.2
32	SCHANG G , ONGARO L , BRÛLÉ E , et al. Transcription factor GATA2 may potentiate follicle-stimulating hormone production in mice via induction of the BMP antagonist gremlin in gonadotrope cells[J]. J Biol Chem, 2022, 298 (7): 102072. doi: 10.1016/j.jbc.2022.102072
33	ROBBINS D J , PAVLETICH T S , PATIL A T , et al. Linking GATA2 to myeloid dysplasia and complex cytogenetics in adult myelodysplastic neoplasm and acute myeloid leukemia[J]. Blood Adv, 2024, 8 (1): 80- 92. doi: 10.1182/bloodadvances.2023011554
34	ZHANG J Y , HE L , WANG Z W , et al. Decreasing GDF15 promotes inflammatory signals and neutrophil infiltration in psoriasis models[J]. J Invest Dermatol, 2023, 143 (3): 419- 430.e8. doi: 10.1016/j.jid.2022.07.026
35	FABOZZI F , MASTRONUZZI A , CEGLIE G , et al. GATA 2 deficiency: focus on immune system impairment[J]. Front Immunol, 2022, 13, 865773. doi: 10.3389/fimmu.2022.865773
36	MARIN-BEJAR O , ROMERO-MOYA D , RODRIGUEZ-UBREVA J , et al. Epigenome profiling reveals aberrant DNA methylation signature in GATA2 deficiency[J]. Haematologica, 2023, 108 (9): 2551- 2557.
37	SETO E , SHI Y , SHENK T . YY1 is an initiator sequence-binding protein that directs and activates transcription in vitro[J]. Nature, 1991, 354 (6350): 241- 245. doi: 10.1038/354241a0
38	GOPALAKRISHNAN J , TESSNEER K L , FU Y , et al. Variants on the UBE2L3/YDJC autoimmune disease risk haplotype increase UBE2L3 expression by modulating CCCTC-binding factor and YY1 binding[J]. Arthritis Rheumatol, 2022, 74 (1): 163- 173. doi: 10.1002/art.41925
39	KHACHIGIAN L M . The Yin and Yang of YY1 in tumor growth and suppression[J]. Int J Cancer, 2018, 143 (3): 460- 465. doi: 10.1002/ijc.31255

名称Name	上游引物(5′→3′) Forward primer	下游引物(5′→3′) Reverse primer	产物长度/bp Length	退火温度/℃ Annealing temperature
P1	TGGGCAGGAGCCAGACTAAGC	AAGGGGATGGTACGCATGGAG	668	63.2
P2	CGGCTAGCGGAAATCCTGACCCCAATATGAC	CAGCTGATTTCATCGAGCAAACCCC	847	62.0
P3	GGCTAGCGCTCCACTGAATCACTCCTT	TCTGGACCAGGCGGTGCACTAAACT	658	62.0
P4	GGCTAGCGCTGCCTGGTATCCGTTTC	CCTCGAGATAGGGCCTTTTGCGTTCGATCC	729	62.0
P5	CAGCAGCATACACGCTCCTGCG	GCTGAGCCGGCCAGCAGCT	294	66.6
P6	ATCCTGACCCCAATATGACTC	ATGGTACGCATGGAGGATC	198	55.3
P7	ATTGGTCATCACTTCATGGCTTTCT	CATATTGTCAAGGCTTTGCCTTCAT	317	60.0

软件分析Software analysis	网站Website
启动子序列的特征分析Characteristic analysis of promoter sequences	https://services.healthtech.dtu.dk/services/Promoter-2.0/
重复元件分析Repeat element analysis	https://www.repeatmasker.org/cgi-bin/WEBRepeatMasker
顺式作用元件分析Analysis of cis-acting elements	https://www.dna.affrc.go.jp/PLACE/?action=newplace
CpG岛位点分析CpG island site analysis	http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi
转录因子结合预测Transcription factor binding prediction	http://jaspar.generec.net/
转录因子结合预测Transcription factor binding prediction	http://bioinfo.life.hust.edu.cn/AnimalTFDB4/#/TFBS_Predict

物种Species	相似性/% Similarity	物种Species	相似性/% Similarity
羚羊Budorcas taxicolor	99.03	绵羊Ovis aries	98.64
普通牛Bos taurus	96.81	瘤牛Bos indicus	91.61
猪Sus scrofa	86.87	马Equus caballus	86.51
雪貂Mustela putorius furo	82.71	老虎Panthera tigris	80.35
骆驼Camelus dromedarius	76.47	双峰驼Camelus bactrianus	74.47
蓝鲸Balaenoptera musculus	71.44	人Homo sapiens	68.23
狗Canis lupus familiaris	64.19	羊驼Vicugna pacos	62.73
鸭嘴兽Ornithorhynchus anatinus	62.44	大熊猫Ailuropoda melanoleuca	61.76
家鼠Mus musculus	53.87	鸡Gallus gallus	42.02
斑马鱼Danio rerio	38.34

SNP位点SNP site	基因型频率(样本数) Genotype frequency (sample number)	等位基因频率Allele frequency	Ho	He	Ne	PIC	χ² (HWE)
SNP1 (g.30667970T＞C)	TT：0.365(73)	T：0.575	0.511	0.489	1.956	0.369	3.957 (P＞0.05)
	TC：0.420(84)	C：0.425
	CC：0.215(43)
SNP2 (g.30668540T＞C)	GG：0.505(101)	G：0.723	0.599	0.401	1.669	0.321	1.439 (P＞0.05)
	TG：0.435(87)	T：0.278
	TT：0.060(12)
SNP3 (g.30668664C＞T)	CC：0.800(160)	C：0.900	0.820	0.180	1.220	0.164	2.469 (P＞0.05)
	CT：0.200(40)	T：0.100
	TT：0.000(0)

SNP位点SNP site	LD系数(D’) LD coefficient	相关系数(r²) Correlation coefficient
SNP1/SNP2	0.942	0.252
SNP1/SNP3	1.000	0.150
SNP2/SNP3	0.910	0.035

海南黑山羊ATG16L2基因启动子区多态性研究

Polymorphism Analysis of the ATG16L2 Gene Promoter Region in Hainan Black Goat

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 13

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价

单倍型名称Haplotype name	单倍型Haplotype	频率F Frequency F
H1	CGC	0.321
H2	TGC	0.304
H3	TTC	0.271
H4	CGT	0.097

[1]	王选艺, 孙亚伟, 龙雨薇, 王俪颖, 周渝新, 李娜, 马雪连, 赵红琼, 姚刚. 屡配不孕母牛FOXP3、FSHR、FMR1基因多态性与生殖激素相关性分析[J]. 畜牧兽医学报, 2024, 55(6): 2727-2740.
[2]	周扬, 吴炜姿, 曹伟胜, 王福广, 许秀琼, 钟文霞, 吴立炀, 叶健, 卢受昇. 基于Nanopore测序技术的非洲猪瘟病毒全基因组测序方法建立[J]. 畜牧兽医学报, 2024, 55(5): 2080-2089.
[3]	曹玉珠, 邢雨欣, 马乘霖, 管宏波, 贾其辉, 康相涛, 田亚东, 李转见, 刘小军, 李红. 鸡FGF6基因生物学特性及其多态性与经济性状的关联分析[J]. 畜牧兽医学报, 2024, 55(4): 1536-1550.
[4]	杨杨, 余乾, 刘昱成, 杨华, 赵卓, 王立民, 周平, 杨庆勇, 代蓉. 绵羊MYL基因家族的鉴定与组织表达分析[J]. 畜牧兽医学报, 2024, 55(4): 1551-1564.
[5]	田睿, 徐思翔, 谢烽, 刘广锦, 王刚, 李庆霞, 代蕾, 谢国信, 张琼文, 陆亚警, 王光文, 王金秀, 张炜. 黄牛源产气荚膜梭菌分离株基因组的生物信息学分析[J]. 畜牧兽医学报, 2024, 55(4): 1707-1715.
[6]	赖婉仪, 陶欣月, 杨庚新, 余文莉, 李树静, Tahir Usman, 俞英. 奶牛乳房健康基因检测芯片在中国荷斯坦牛及巴基斯坦本地奶牛群中的应用研究[J]. 畜牧兽医学报, 2024, 55(10): 4489-4499.
[7]	王栋梁, 任静, 郝琴琴, 李鹏飞. 牛CART基因核心启动子鉴定及转录调控分析[J]. 畜牧兽医学报, 2023, 54(9): 3689-3699.
[8]	路畅, 董磊, 张万锋, 高鹏飞, 郭晓红, 蔡春波, 曹果清, 李步高. 基于全基因组重测序对晋汾白猪单核苷酸多态性位点鉴定和筛选[J]. 畜牧兽医学报, 2023, 54(7): 2761-2771.
[9]	杜小迪, 侯巍, 苏中华, 马青梅, 何雪, 华瑞其, 阳爱国, 杨光友. 细粒棘球绦虫泛素结合酶基因家族的生物信息学及表达分析[J]. 畜牧兽医学报, 2023, 54(6): 2605-2618.
[10]	孟秋赤, 卢光玉, 陈定双, 林亚秋, 王瑞龙, 钟朝崧, 王永, 刘伟, 王友利, 李艳艳, 李志雄. 山羊GPR35基因表达特性分析及对皮下脂肪细胞分化作用的研究[J]. 畜牧兽医学报, 2023, 54(12): 4993-5007.
[11]	徐婷婷, 齐芬芳, 黄世会, 牛熙, 李升, 冉雪琴, 王嘉福, 谢健. 香猪MAP3K4基因结构变异多态性和基因表达研究[J]. 畜牧兽医学报, 2023, 54(12): 5046-5055.
[12]	宋鹏琰, 王思伟, 岳巧娴, 张寅梁, 陈晓勇, 周荣艳. 绵羊miR-200b启动子鉴定及其对卵泡颗粒细胞线粒体功能的影响[J]. 畜牧兽医学报, 2023, 54(12): 5066-5076.
[13]	陈诚, 乔西波, 孙亿, 康丽, 姜运良. 琅琊鸡FSHR基因-868位点的多态性及对产蛋性能的遗传效应研究[J]. 畜牧兽医学报, 2023, 54(11): 4560-4568.
[14]	杨洋, 周子薇, 张京一, 杨硕, 王博宇, 葛楠, 林叶, 侯晓明. 奶牛SP1基因结构及对乳脂合成功能的初步分析[J]. 畜牧兽医学报, 2022, 53(9): 2970-2981.
[15]	岑鑫, 阳亭亭, 赵尊福, 文永平, 张焕容. 沙门菌烈性噬菌体的分离鉴定、生物学特性及基因组分析[J]. 畜牧兽医学报, 2022, 53(8): 2677-2688.